Investigation on toxic effects of organophosphate pesticide to endocrine organs related to reproductive system, and identification of target molecules of the chemicals in these organs.

• Project/Area Number: 21710069
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Saitama Medical University
• Principal Investigator: HIROSAWA Narumi Saitama Medical University, 医学部, 助教 (40327060)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 有機リン系農薬 / 生殖毒性 / プロテオーム

Research Abstract

I carried out exposure experiments to the mature male rats in order to investigate the effects of the fenitrothion on endocrine organs. As the result, in the 30 mg/kg/day administrated group, adrenal and pituitary glands showed the increase in the weight. In addition, testosterone and ACTH blood concentration were increased by the administration of fenitrothion. I quantitatively compared the expressed proteins on the each glands of administrated with control groups and performed identifications of the interest proteins based on the proteomic method.

Research Products

• [Journal Article] Bisphenol A in combination with TNF-alpha selectively induces Th2 cell-promoting dendritic cells in vitro with an estrogen-like activity. (2010)
  • Author(s): Guo H, Liu T, Uemura Y, Jiao S, Wang D, Lin Z, Narita Y, Suzuki M, Hirosawa N, Ichihara Y, Ishihara O, Kikuchi H, Sakamoto Y, Senju S, Zhang Q, Ling F.
  • Journal Title: Cell Mol Immunol. 7(3) Pages: 227-234
  • Peer Reviewed
• [Journal Article] Bisphenol A in combination with TNF-alpha selectively induces Th2 cell-promoting dendritic cells in vitro with an estrogen-like activity. (2010)
  • Author(s): Guo H
  • Journal Title: Cell Mol Immunol. Volume: 7(3) Pages: 227-234
  • Peer Reviewed
• [Journal Article] 環境有機リン暴露の加齢、視機能に及ぼす影響 (2009)
  • Author(s): 坂本安、米谷新、廣澤成美
  • Journal Title: 埼玉医科大学雑誌 第36巻第1号 Pages: 85-86
• [Journal Article] Cytokine-dependent modification of IL-12p70 and IL-23 balance in dendritic cells by ligand activation of Valpha24 invariant NKT cells. (2009)
  • Author(s): Uemura Y, Liu TY, Narita Y, Suzuki M, Nakatsuka R, Araki T, Matsumoto M, Iwai LK, Hirosawa N, Matsuoka Y, Murakami M, Kimura T, Hase M, Kohno H, Sasaki Y, Ichihara Y, Ishihara O, Kikuchi H, Sakamoto Y, Jiao SC, Senju S, Sonoda Y.
  • Journal Title: J Immunol. 183(1) Pages: 201-208
  • Peer Reviewed
• [Journal Article] Cytokine-dependent modification of IL-12p70 and IL-23 balance in dendritic cells by ligand activation of Valpha24 invariant NKT cells. (2009)
  • Author(s): Uemura Y.
  • Journal Title: J Immunol. 183(1) Pages: 201-208
  • Peer Reviewed
• [Presentation] フェニトロチオンとその代謝産物の生体への影響調査 (2010)
  • Author(s): 坂本安
  • Organizer: フォーラム2010 衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
• [Presentation] Modification of IL-12p70 and IL-23 balance in dendritic cells by ligand activation of Valpha24 invariant NKT cells (2009)
  • Author(s): 鈴木元晴
  • Organizer: 第68回 日本癌学会学術総会
  • Place of Presentation: 横浜