| Project/Area Number |
21710235
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MORITA Hiroyuki The University of Tokyo, 大学院・薬学系研究科, 助教 (20416663)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 生合成 / ポリケタイド合成酵素 / タンパク工学 / ポリフェノール / アルカロイド / クルクミン / テトラミン酸 / ベンザルアセトン / カルコン |
|---|
| Research Abstract |
We solved crystal structures of benzalacetone synthase (BAS) from Rheum palmatum, curcuminoid synthase (CUS) from Oryza sativa and polyketide synthase (HsPKS) from Hupersia serrata. Furthermore, by carrying out the structure-based site-directed mutagenesis studies, we succeeded to add a new function against CUS and HsPKS, respectively. We also revealed that BAS produces tetramic acid derivatives from a condensation of aminoacyl-CoA with malonyl-CoA.
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