High pressure crystallization and X-ray crystal structure analysis of Proteins

• Project/Area Number: 21740234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Condensed matter physics I
• Research Institution: Ritsumeikan University
• Principal Investigator: YAMASHITA Motoi Ritsumeikan University, 総合理工学研究機構, ポストドクトラルフェロー (30424998)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: タンパク質 / 高圧 / 結晶化 / 高エネルギー構造 / ユビキチン / 分子ステップ / 界面モルフォロジー / 結晶成長 / 蛋白質 / 生体分子 / 生物物理

Research Abstract

Under high pressure, population of physiologically important conformers (high-energy state conformer) of proteins drastically increases. In this work, we successfully developed techniques to form high-energy state-rich crystal phase of proteins by crystallizing proteins under high pressures.

Research Products

• [Journal Article] The effect of pressure on the phase transition behavior of tridecane, pentadecane, and heptadecane : A Fourier transform infrared spectroscopy study (2011)
  • Author(s): Motoi Yamashita, Atsushi Hirao, Minoru Kato
  • Journal Title: THE JOURNAL OF CHEMICAL PHYSICS 134
  • Peer Reviewed
• [Journal Article] The effect of pressure on the phase transition behavior of tridecane, pentadecane, and heptadecane : A Fourier transform infrared spectroscopy study (2011)
  • Author(s): Motoi Yamashita, Atsushi Hirao, Minoru Kato
  • Journal Title: THE JOURNAL OF CHEMICAL PHYSICS Volume: 134
  • Peer Reviewed
• [Presentation] DDetection of Entropic Effect of Polymer Chain Folding Directions : Crystal Growth of isotactic Polybutene-1 Tetragonal Phase (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-10
• [Presentation] Detection of Entropic Effect of Polymer Chain Folding Directions : Crystal Growth of isotactic Polybutene-1 Tetragonal Phase (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-10
• [Presentation] High Pressure Rotator Phase of n-Pentadecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] High Pressure Rotator Phase of n-Tridecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] High Pressure Rotator Phase of n-Heptadecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Tridecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Pentadecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Heptadecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-08
• [Presentation] High Pressure Rotator Phase of n-Pentadecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Presentation] High Pressure Rotator Phase of n-Tridecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Presentation] High Pressure Rotator Phase of n-Heptadecane Studied by FT-IR : Factor Group Splitting (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Tridecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Pentadecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Presentation] Defect population in the High Pressure Rotator Phase of n-Heptadecane Studied by FT-IR (2010)
  • Author(s): Motoi Yamashita
  • Organizer: The 16th International Conference on Crystal Growth (ICCG 16)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-08
• [Remarks] ホームページ等