Development of highly-sensitive time-resolved IR spectrometer to study the proton-pumping mechanisms of biomolecules

• Project/Area Number: 21750022
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical chemistry
• Research Institution: University of Hyogo
• Principal Investigator: KUBO Minoru University of Hyogo, 大学院・生命理学研究科, 助教 (90392878)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 赤外分光法 / 時間分解振動分光法 / タンパク質 / プロトンポンプ / チトクロム酸 / 化酵素 / フェムト秒レーザー / ミトコンドリア / シトクロム酸化酵素 / 光反応

Research Abstract

A new highly-sensitive IR spectrometer that can measure nanosecond time-resolved IR spectra of proteins under physiological conditions has been developed to study the proton-pumping mechanisms of proteins. The spectrometer has been applied to bovine cytochrome c oxidase. The dynamic regulation mechanism of water channel gate of this protein is elucidated.

Research Products

• [Journal Article] Spectroscopic identification of reactive porphyrin motions. (2011)
  • Author(s): Barabanschikov, A., Demidov, A., Kubo, M., Champion, P. M., Zhao, J., Sturhahn, W., Alp, E.E., Sage, J.T.
  • Journal Title: J.Chem.Phys. In press.
  • Peer Reviewed
• [Journal Article] Spectroscopic and computational characterization of Cu^<II>-OOR (R=H or Cumyl) complexes bearing a Me_6-tren ligand. (2011)
  • Author(s): Choi, Y.J., Cho, K.-B., Kubo, M., Ogura, T., Karlin, K.D., Cho, J., Nam, W.
  • Journal Title: Dalton Trans. (Special Issue, New Talent Asia). Vol.40 Pages: 2234-2241
  • Peer Reviewed
• [Journal Article] Differences in coordination states of substituted tyrosine residues and quaternary structures among hemoglobin M probed by resonance Raman spectroscopy. (2010)
  • Author(s): Aki, Y., Nagai, M., Nagai, Y., Imai, K., Aki, M., Sato, A., Kubo, M., Nagatomo, S., Kitagawa, T.
  • Journal Title: J.Biol.Inorg.Chem. Vol.15 Pages: 147-158
  • Peer Reviewed
• [Journal Article] Ultrafast dynamics of diatomic ligand binding to nitrophorin 4. (2010)
  • Author(s): Benabbas, A., Ye, X., Kubo, M., Zhang, Z., Maes, E.M., Montfort, W.R., Champion, P.M.
  • Journal Title: J.Am.Chem.Soc. Vol.132 Pages: 2811-2820
  • Peer Reviewed
• [Journal Article] Water as an oxygen source : Synthesis, characterization, and reactivity studies of a mononuclear nonheme manganese(IV) oxo complex. (2010)
  • Author(s): Sawant, S.C., Wu, X., Cho, J., Cho, K.-B., Kim, S.H., Seo, M.S., Lee, Y.-M., Kubo, M., Ogura, T., Shaik, S., Nam, W.
  • Journal Title: Angew.Chem.Int.Ed. Vol.49 Pages: 8190-8194
  • Peer Reviewed
• [Journal Article] Synthesis, structural and spectroscopic characterization, and reactivities of mononuclear cobalt (III)-peroxo complexes. (2010)
  • Author(s): Cho, J., Sarangi, R., Kang, H. Y., Lee, J. Y., Kubo, M., Ogura, T., Solomon, E.I., Nam, W.
  • Journal Title: J.Am.Chem.Soc. Vol.132 Pages: 16977-16986
  • Peer Reviewed
• [Journal Article] Reversible O-O bond cleavage and formation between Mn(IV)-peroxo and Mn(V)-oxo corroles. (2010)
  • Author(s): Kim, S.H., Park, H., Seo, M. S., Kubo, M., Ogura, T., Klajn, J., Gryko, D.T., Valentine, J.S., Nam, W.
  • Journal Title: J.Am.Chem.Soc. Vol.132 Pages: 14030-14032
  • Peer Reviewed
• [Journal Article] A square-planar Ni (II) complex with an N_2S_2 donor set similar to the active centre of nickel-containing superoxide dismutase and its reaction with superoxide. (2010)
  • Author(s): Nakane, D., Kuwasako, S., Tsuge, M., Kubo, M., Funahashi, Y., Ozawa, T., Ogura, T., Masuda, H.
  • Journal Title: Chem.Commun. Vol.46 Pages: 2142-2144
  • Peer Reviewed
• [Journal Article] Nickel (II) complexes of TPA ligands with 6-phenyl substituents Ph^nTPA. Structure and H_2O_2-reactivity.(Selected Paper). (2010)
  • Author(s): Tano, T., Doi, Y., Inosako, M., Kunishita, A., Kubo, M., Ishimaru, H., Ogura, T., Sugimoto, H., Itoh, S.
  • Journal Title: Bull.Chem.Soc.Jpn. Vol.83 Pages: 530-538
  • Peer Reviewed
• [Journal Article] Geometric and electronic structure and reactivity of a mononuclear "side-on" nickel (III)-peroxo complex. (2009)
  • Author(s): Cho, J., Sarangi, R., Annaraj, J., Kim, S. Y., Kubo, M., Ogura, T., Solomon, E.I., Nam, W.
  • Journal Title: Nature Chem. Vol.1 Pages: 568-572
  • Peer Reviewed
• [Journal Article] Water as an oxygen source in the generation of mononuclear nonheme iron (IV)-oxo complexes. (2009)
  • Author(s): Lee, Y.-M., Dhuri, S.N., Sawant, S.C., Cho, J., Kubo, M., Ogura, T., Fukuzumi, S., Nam, W.
  • Journal Title: Angew.Chem.Int.Ed. Vol.48 Pages: 1803-1806
  • Peer Reviewed
• [Journal Article] New oxidation reactivity of a bis(μ-oxo)-dinickel(III) complex : Arene hydroxylation of the supporting ligand. (2009)
  • Author(s): Honda, K., Cho, J., Matsumoto, T., Roh, J., Furutachi, H., Tosha, T. Kubo, M., Fujinami, S., Ogura, T., Kitagawa, T., Suzuki, M.
  • Journal Title: Angew.Chem.Int.Ed. Vol.48 Pages: 3304-3307
  • Peer Reviewed
• [Journal Article] (∝-η^2:η^2-disulfido) dinickel (II) complexes supported by 6-methyl-TPA ligands. (2009)
  • Author(s): Inosako, M., Kunisita, A., Kubo, M., Ogura, T., Sugimoto, H., Itoh, S.
  • Journal Title: Dalton Trans.(Selected Paper for Cover Picture). Pages: 9410-9417
  • Peer Reviewed
• [Journal Article] Ni(II)/H_2O_2 reactivity in bis[(pyridin-2-yl)methyl]amine tridentate ligand system. Aromatic hydroxylation reaction by bis(∝-oxo)dinickel (III) complex. (2009)
  • Author(s): Kunishita, A., Doi, Y., Kubo, M., Ogura, T., Sugimoto, H., Itoh, S.
  • Journal Title: Inorg.Chem. Vol.48 Pages: 4997-5004
  • Peer Reviewed
• [Journal Article] Mononuclear copper(II)-superoxo complexes that mimic the structure and reactivity of the active centers of PHM and DβM. (2009)
  • Author(s): Kunishita, A., Kubo, M., Sugimoto, H., Ogura, T., Sato, K., Takui, T., Itoh, S.
  • Journal Title: J.Am.Chem.Soc. Vol.131 Pages: 2788-2789
  • Peer Reviewed
• [Presentation] フェムト秒レーザーを用いた高感度時間分解赤外分光装置の開発:酵素反応の生理条件下での測定を目指して (2010)
  • Author(s): 久保稔
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2010-09-20
• [Presentation] フェムト秒レーザーを用いた高感度時間分解赤外分光装置の開発:酵素反応の生理条件下での測定を目指して (2010)
  • Author(s): 久保稔
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内北キャンパス(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] 高輝度フェムト秒レーザーを用いた時間分解赤外分光装置の開発 (2010)
  • Author(s): 久保稔
  • Organizer: 第4回分子科学討論会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2010-09-14
• [Presentation] 高輝度フェムト秒レーザーを用いた時間分解赤外分光装置の開発 (2010)
  • Author(s): 久保稔
  • Organizer: 第4回分子科学討論会
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府)
  • Year and Date: 2010-09-14
• [Presentation] フェムト秒レーザーを用いた高感度赤外分光装置の開発 (2010)
  • Author(s): 久保稔
  • Organizer: 生命科学系GCOEネットワークフォーラム2010
  • Place of Presentation: メルパルク京都(京都)
  • Year and Date: 2010-02-21