Development of Microfluidic Device for Detection of Oncogenes
Project/Area Number |
21750080
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Analytical chemistry
|
Research Institution | Doshisha University |
Principal Investigator |
HASHIMOTO Masahiko Doshisha University, 理工学部, 准教授 (20439251)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | チップ分析 / 機器分析 / 生体成分 / バイオセンサー / 試料処理 / DNA / 癌遺伝子 / 点突然変異 / 生体分析 |
Research Abstract |
The author has developed a microfluidic analytical system, named "flow-through LDR/sandwich hybridization assay", capable of detecting low abundant point mutations where ligase detection reaction (LDR) was employed in conjunction with functional particles of nano- to micro-scale. The author successfully demonstrated its ability to detect one mutant sequence in 100 normal sequences with the present system. In addition, the post-LDR procedures could be processed at a relatively high speed (total required time, ~4.4 min).
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Report
(3 results)
Research Products
(64 results)