| Project/Area Number |
21750180
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Yasuda Women's University |
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Principal Investigator |
TAKANORI Kubo Yasuda Women's University, 薬学部・薬学科, 助教 (90435751)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイオテクノロジー / 核酸 / 発現抑制 / RNA干渉 / 遺伝子発現抑制 / コンジュゲート型2本鎖RNA / 脂質 / ペプチド / DDS |
|---|
| Research Abstract |
RNAi technology is a powerful tool for suppressing gene expression in mammalian cells. In this study, we developed siRNAs conjugated with bioactive molecules in order to enhance potency of RNAi. The siRNAs conjugated with bioactive molecules had potent RNAi efficacy, higher nuclease stability, and efficient membrane permeability. Moreover, excellent gene-silencing efficacies of siRNAs conjugated with bioactive molecules were found in a subcutaneously implanted tumor mouse model. Our developed siRNAs conjugated with bioactive molecules are among the promising candidates for a new generation of modified-siRNAs that can solve the many problems associated with RNAi.
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