| Project/Area Number |
21750182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KUBOTA Takeshi The Institute of Physical and Chemical Research, 岡本独立主幹研究ユニット, 基幹研究所研究員 (40455340)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 蛍光イメージング / RNA / 人工核酸 / マイクロインジェクション / 蛍光核酸 / 人工酸 / ハイブリダイゼーション |
|---|
| Research Abstract |
Probe-tag pair system was applied to develop a technique for visualizing live cell mRNA localization. The probe was a doubly fluorescent dye labeled oligonucleotide to have a fluorescent enhancement property in the presence of the complementary DNA/RNA strand. The tag structure was a complementary sequence to the probe, and it was inserted into a target mRNA strand. The fluorescent probe was microinjected into cells and the tag inserted mRNA was expressed in the same living cells. By using the probe-tag pair, target mRNAs in living cells were successfully visualized.
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