Construction of protein displaying system on the cell surface of Streptomyces species for bio-refinery

• Project/Area Number: 21760640
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: OGINO Chiaki Kobe University, 大学院・工学研究科, 准教授 (00313693)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 放線菌 / 細胞工場 / タンパク質生産 / タンパク生産 / 細胞表層工学

Research Abstract

In this study, by using these functions in Streptomyces, we undertook following three kinds of research topics:
[1] Secretory protein production in culture medium
[2] Protein displaying system on the cell surface of Streptomyces strain
[3] Construction of cell factory for building block production
In first topic, several secretory enzymes such like a transglutaminase and cellulases were well produced by our constructed platform. In second topic, among the membrane protein library from three kinds of Streptomyces genome sequence, we found one kind of anchor protein for protein display. In final topic, we could perform the cinnamic acid production form glucose. In addition, this production level from glucose was attained at 500mg/L of cinnamic acid, and this level is highest production among the previously reported papers.

Research Products

• [Journal Article] Cinnamic acid production using phenylalnine ammonia lyase expressing Streptomyces lividans. (2011)
  • Author(s): Noda, S., Miyazaki, T., Miyoshi, T., Miyake, M., Okai, N., Tanaka, T., Ogino C., Kondo, A.
  • Journal Title: Journal of Industrial Microbiology and Biotechnology. 38(5) Pages: 643-648
• [Journal Article] Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans. (2011)
  • Author(s): Sugimori, D., Tomita, Y., Matsumoto, Y., Ogiono, C.
  • Journal Title: Biotechnology Letters 33(4) Pages: 727-731
• [Journal Article] Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans (2011)
  • Author(s): Sugimori, D., Tomita, Y., Matsumoto, Y., Ogiono, C.
  • Journal Title: Biotechnology Letters Volume: 33(4) Pages: 727-731
  • Peer Reviewed
• [Journal Article] Cinnamic acid production using phenylalnine ammonia lyase expressing Streptomyces lividans (2011)
  • Author(s): Noda, S., Miyazaki, T., Miyoshi, T., Miyake, M., Okai, N., Tanaka, T., Ogino C., Kondo, A.
  • Journal Title: Journal of Industrial Microbiology and Biotechnology Volume: 38(5) Pages: 643-646
  • Peer Reviewed
• [Journal Article] Overproduction of various secretory-form proteins in Streptomyces lividans. (2010)
  • Author(s): Noda, S., Ito, Y., Shimizu, N., Tanaka, T., Ogino, C., Kondo, A.
  • Journal Title: Protein Expression and Purification 73 Pages: 198-202
• [Presentation] Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividans. (2011)
  • Author(s): Shuhei Noda, Miyoshi Takanori, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo.
  • Organizer: 33rd Symposium on Biotechnology for Fuels and Chemicals.
  • Place of Presentation: Seattle, WA, USA
• [Presentation] 放線菌を用いた様々なタンパクの大量分泌系の構築 (2010)
  • Author(s): 野田修平・岡井直子・田中勉・近藤昭彦・荻野千秋
  • Organizer: 化学工学会 第75年会(鹿児島大会)
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島県)
  • Year and Date: 2010-03-18
• [Presentation] Akihiko Kondo.Overproduction of various secretory-form proteins in Streptomyces lividans. (2010)
  • Author(s): Shuhei Noda, Tsutomu Tanaka, Chiaki Ogino.
  • Organizer: YABEC 2010
  • Place of Presentation: Taipei, Taiwan
• [Remarks] ホームページ等
  • URL: http://www2.kobe-u.ac.jp/~akondo/index.html
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/~akondo/index_English.htm
• [Patent(Industrial Property Rights)] 桂皮酸の製造方法 (2010)
  • Inventor(s): 近藤昭彦, 荻野千秋, 岡井直子, 三好孝則, 野田修平
  • Industrial Property Rights Holder: 神戸大学、帝人
  • Filing Date: 2010-12-24