| Project/Area Number |
21760640
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kobe University |
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Principal Investigator |
OGINO Chiaki Kobe University, 大学院・工学研究科, 准教授 (00313693)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 放線菌 / 細胞工場 / タンパク質生産 / タンパク生産 / 細胞表層工学 |
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| Research Abstract |
In this study, by using these functions in Streptomyces, we undertook following three kinds of research topics:
[1] Secretory protein production in culture medium
[2] Protein displaying system on the cell surface of Streptomyces strain
[3] Construction of cell factory for building block production
In first topic, several secretory enzymes such like a transglutaminase and cellulases were well produced by our constructed platform. In second topic, among the membrane protein library from three kinds of Streptomyces genome sequence, we found one kind of anchor protein for protein display. In final topic, we could perform the cinnamic acid production form glucose. In addition, this production level from glucose was attained at 500mg/L of cinnamic acid, and this level is highest production among the previously reported papers.
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