Development of enzymatic oxidation system in liposomes using gas-liquid flow for controlling the enzyme activity

• Project/Area Number: 21760642
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Yamaguchi University
• Principal Investigator: YOSHIMOTO Makoto Yamaguchi University, 大学院・医学系研究科, 准教授 (80322246)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: バイオリアクター / 酵素反応 / 生体機能利用 / ナノバイオ / 化学工学

Research Abstract

The permeabilization of phospholipid bilayer membrane of liposomes to a low molecular mass compound was induced by the liquid shear stress at high temperatures. The catalase-bound liposome encapsulating glucose oxidase was prepared and its permeabilization with respect to glucose and oxygen was induced by the liquid shear stress generated by the gas-liquid flow in an external loop airlift bubble column. The liposomal enzyme system prepared functioned as a reactive and stable catalyst for the oxidation of glucose with decomposition of hydrogen peroxide produced in the bubble column.

Research Products

• [Journal Article] Thermal stabilization of formaldehyde dehydrogenase with nicotinamide adenine dinucleotide (2011)
  • Author(s): M.Yoshimoto, T.Yamashita, S.Kinoshita
  • Journal Title: Enzyme Microb.Technol. in press
  • Peer Reviewed
• [Journal Article] Stability and reactivity of liposome-encapsulated formate dehydrogenase and cofactor system in carbon dioxide gas-liquid flow. (2010)
  • Author(s): M.Yoshimoto, T.Yamashita, T.Yamashiro
  • Journal Title: Biotechnol.Prog. 26 Pages: 1047-1053
  • Peer Reviewed
• [Journal Article] A biosensor composed of glucose oxidase-containing liposomes and MnO2-based layered nanocomposite. (2010)
  • Author(s): M.Yoshimoto, C.Iida, A.Kariya, N.Takaki, M.Nakayama
  • Journal Title: Electroanalysis 22 Pages: 653-659
  • Peer Reviewed
• [Journal Article] Catalase-conjugated liposomes encapsulating glucose oxidase for controlled oxidation of glucose with decomposition of hydrogen peroxide produced. (2010)
  • Author(s): M.Yoshimoto, N.Takaki, M.Yamasaki
  • Journal Title: Coll.Sur.B 79 Pages: 403-408
  • Peer Reviewed
• [Journal Article] Stabilization of formate dehydrogenase from Candida boidinii through liposome-assisted complexation with cofactors. (2010)
  • Author(s): M.Yoshimoto, R.Yamasaki, M.Nakao, T.Yamashita
  • Journal Title: Enzyme Microb.Technol. 46 Pages: 588-593
  • Peer Reviewed
• [Journal Article] Gas-liquid flow-induced permeabilization of phospholipid bilayer membranes for regulating catalytic performance of liposome-encapsulated bovine liver catalase. (2010)
  • Author(s): M.Yoshimoto, T.Natsume, N.Matsumoto, H.Sakamoto
  • Journal Title: Coll.Surf.A 360 Pages: 63-68
  • Peer Reviewed
• [Journal Article] Catalase-conjugated liposomes encapsulating glucose oxidase for controlled oxidation of glucose with decomposition of hydrogen peroxide produced (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Journal Title: Colloids and Surfaces B Volume: 79 Pages: 403-408
  • Peer Reviewed
• [Journal Article] Stability and reactivity of liposome-encapsulated formate dehydrogenase and cofactor system in carbon dioxide gas-liquid flow (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Journal Title: Biotechnology Progress Volume: 26 Pages: 1047-1053
  • Peer Reviewed
• [Journal Article] A biosensor composed of glucose oxidase-containing liposomes and MnO_2-based layered nanocomposite (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Journal Title: Electroanalysis 22 Pages: 653-659
  • Peer Reviewed
• [Journal Article] Gas-liquid flow-induced permeabilization of phospholipid bilayer membranes for regulating catalytic performance of liposome-encapsulated bovine liver catalase (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Journal Title: Colloids and Surfaces A 360 Pages: 63-68
  • Peer Reviewed
• [Journal Article] Stabilization of formate dehydrogenase from Candida boidinii through liposome-assisted complexation with cofactors (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Journal Title: Enzyme and Microbial Technology 46 Pages: 588-593
  • Peer Reviewed
• [Presentation] 補酵素-蟻酸脱水素酵素間結合に及ぼすリポソーム膜表面への補酵素複合化の影響 (2011)
  • Author(s): 吉本誠, ら
  • Organizer: 化学工学会第76年会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-03-23
• [Presentation] リポソーム膜の流動性に及ぼす膜内Mg-Chlorophyll a含有量の影響 (2010)
  • Author(s): 吉本誠, ら
  • Organizer: 膜シンポジウム2010
  • Place of Presentation: 京都大学
  • Year and Date: 2010-11-19
• [Presentation] 高活性を発現するリポソーム修飾複合酵素を用いるグルコース酸化反応操作 (2010)
  • Author(s): 高木規行, ら
  • Organizer: 化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2010-10-24
• [Presentation] 遊離脱水素酵素の失活特性に基づく高安定性を有するリポソーム内封入酵素の調製 (2010)
  • Author(s): 山下孝幸, ら
  • Organizer: 化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2010-10-24
• [Presentation] 固定化補酵素リポソームの調製と機能に及ぼす脂質膜内の架橋剤分子種と密度の影響 (2010)
  • Author(s): 國廣信貴, ら
  • Organizer: 化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2010-10-24
• [Presentation] リポソーム内水相における高い熱安定性を示す蟻酸脱水素酵素-補酵素複合体の形成 (2010)
  • Author(s): 吉本 誠, 山下孝幸, 山城卓哉
  • Organizer: 化学工学3支部合同徳島大会
  • Place of Presentation: 徳島,徳島大学
  • Year and Date: 2010-10-23
• [Presentation] リポソニム内水相における高い熱安定性を示す蟻酸脱水素酵素-補酵素複合体の形成 (2010)
  • Author(s): 吉本誠, ら
  • Organizer: 化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2010-10-23
• [Presentation] Preparation and physicochemical properties of Mg-chlorophyll a-incorporated phospholipid bilayer membranes (2010)
  • Author(s): Makoto Yoshimoro, et al.
  • Organizer: The 8th International Symposium on membrane Stress Biotechnology
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-22
• [Presentation] Oxidation of glucose with decomposition of hydrogen peroxide produced catalyzed by liposome-encapsulated glucose oxidase and membrane-bound catalase (2010)
  • Author(s): Makoto Yoshimoto, et al.
  • Organizer: 7th Symposium on Engineering Science of Liposome
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-18
• [Presentation] カタラーゼ複合化リポソーム膜を利用するグルコースオキシダーゼ反応速度の制御 (2010)
  • Author(s): 山崎 未来, 高木規行, 吉本 誠
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都,同志社大学
  • Year and Date: 2010-09-06
• [Presentation] クロロフィル含有リポソーム膜系の電子移動に及ぼす脂質膜の流動性と表面電荷の影響 (2010)
  • Author(s): 古谷武宏, ら
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学
  • Year and Date: 2010-09-06
• [Presentation] カタラーゼ複合化リポソーム膜を利用するグルコースオキシダーゼ反応速度の制御 (2010)
  • Author(s): 山崎未来, ら
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学
  • Year and Date: 2010-09-06
• [Presentation] リポソーム内封入酵素の安定性と反応性に及ぼすエアリフト型気泡塔内気液流動の影響 (2010)
  • Author(s): 夏目友誉, ら
  • Organizer: 分離技術会年会2010
  • Place of Presentation: 明治大学
  • Year and Date: 2010-06-04
• [Presentation] 炭酸ガスを通気したエアリフト型気泡塔におけるリポソーム系蟻酸脱水素酵素反応操作 (2010)
  • Author(s): 山城卓哉, ら
  • Organizer: 分離技術会年会2010
  • Place of Presentation: 明治大学
  • Year and Date: 2010-06-04
• [Presentation] 気液二相流中における脂質膜の透過性変化に基づくリポソーム系酵素反応速度の制御 (2010)
  • Author(s): 吉本 誠, 夏目友誉
  • Organizer: 分離技術会年会2010
  • Place of Presentation: 東京,明治大学
  • Year and Date: 2010-06-03
• [Presentation] 気液二相流中における脂質膜の透過性変化に基づくリポソーム系酵素反応速度の制御 (2010)
  • Author(s): 吉本誠, ら
  • Organizer: 分離技術会年会2010
  • Place of Presentation: 明治大学
  • Year and Date: 2010-06-03
• [Presentation] 層状マンガン酸化物薄膜におけるリポソーム内封入グルコースオキシダーゼ反応の電気化学的検出 (2010)
  • Author(s): 高木規行, ら
  • Organizer: 第71回分析化学討論会
  • Place of Presentation: 島根大学
  • Year and Date: 2010-05-15
• [Presentation] エアリフトに懸濁させた蟻酸脱水素酵素封入リポソームの活性に及ぼす炭酸ガス通気の影響 (2010)
  • Author(s): 吉本 誠, 山下孝幸, 山城卓哉
  • Organizer: 化学工学会第75年会
  • Place of Presentation: 鹿児島,鹿児島大学
  • Year and Date: 2010-03-19
• [Presentation] エアリフトに懸濁させた蟻酸脱水素酵素封入リポソームの活性に及ぼす炭酸ガス通気の影響 (2010)
  • Author(s): 吉本誠, ら
  • Organizer: 化学工学会第75年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2010-03-19
• [Presentation] Stability and reactivity of liposomal formate dehydrogenase suspended in carbon dioxide gas-liquid flow in a bioreactor (2010)
  • Author(s): Makoto Yoshimoto
  • Organizer: 5th Choshu-London Memorial Symposium in Chemistry
  • Place of Presentation: 山口大学
  • Year and Date: 2010-03-08
• [Presentation] リポソーム膜/補酵素共存系による蟻酸脱水素酵素の安定化 (2009)
  • Author(s): 吉本誠, ら
  • Organizer: 7th Membrane Stress Biotechnology Symposium
  • Place of Presentation: 関西大学
  • Year and Date: 2009-12-14
• [Presentation] Preparation and catalytic properties of glucose oxidase encapsulated in catalase-conjugated liposomes for effective oxidation of glucose. (2009)
  • Author(s): M.Yoshimoto, N.Takaki
  • Organizer: Asia Pacific Biochemical Engineering Conference
  • Place of Presentation: 神戸 神戸国際会議場
  • Year and Date: 2009-11-25
• [Presentation] Preparation and catalytic properties of glucose oxidase encapsulated in catalase-conjugated liposomes for effective oxidation of glucose (2009)
  • Author(s): Makoto Yoshimoto, et al.
  • Organizer: Asia Pacific Biochemical Engineering Conference 2009
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-11-25
• [Presentation] Chlorophyll aを複合化した脂質二分子膜の調製と光誘起電子移動特性 (2009)
  • Author(s): 國廣信貴, ら
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-09-18
• [Presentation] リポソームに構築した複合酵素系による過酸化水素分解を伴うグルコース酸化反応 (2009)
  • Author(s): 高木規行, ら
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-09-18
• [Presentation] 蟻酸脱水素酵素/補酵素系のリポソーム内封入による安定化と炭酸ガス還元活性 (2009)
  • Author(s): 山下孝幸, ら
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-09-18
• [Book] Enzyme Stabilization and Immobilization: Methods and Protocols, Methods in Molecular Biology, (S.D.Minteer ed.) (2011)
  • Author(s): M.Yoshimoto
  • Publisher: Stabilization of enzymes through encapsulation in liposomes (Chapter 2).
• [Book] Stabilization of enzymes through encapsulation in liposomes (Chapter 2), Enzyme Stabilization and Immobilization : Methods and Protocols, Methods in Molecular Biology (S.D.Minteer ed.), Vol.679 (2011)
  • Author(s): Makoto Yoshimoto, et al.
  • Total Pages: 10
  • Publisher: Humana Press
• [Remarks] ホームページ等