Establishment of chicken pluripotent stem cells with germ line competency

Research Project

Project/Area Number	21760643
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biofunction/Bioprocess
Research Institution	Kyushu University
Principal Investigator	KAWABE Yoshinori Kyushu University, 工学研究院, 助教 (30448401)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	多能性幹細胞 / トランスジェニック鳥類 / キメラニワトリ / フィーダー細胞 / ニワトリ / 生殖系列キメラ / 生体バイオリアクター / レトロウイルスベクター / バイオ医薬品 / 生殖系列キメラニワトリ / バイオリアクター
Research Abstract	To establish chicken induced pluripotent stem cells, some chicken homologues to regulate pluripotency could be obtained. Retroviral vectors encoding their homologues were produced and infected into STO cells. Chicken blastodermal cells were cultured on genetically modified STO cells and the undifferentiated state was investigated. Chicken embryonic fibroblasts transducted by some retroviral vectors were evaluated by RT-PCR and AP staining. These results suggest that further improvement to induce chicken pluripotent stem cells is required.

Report

(3 results)