Molecular evolutionary basis of pollen tube guidance mechanism for species recognition and fertilization

• Project/Area Number: 21770041
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: KANAOKA Masahiro 名古屋大学, 理学研究科, 助教 (10467277)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 花粉管ガイダンス / 種分化 / 植物有性生殖 / トレニア / シグナル伝達

Research Abstract

Here I investigated the molecular diversity of LURE, which is important for pollen tube guidance in Torenia. LURE1 althologues were conserved in the Torenia species, but its protein sequences were diverged. LURE1 proteins attracted pollen tubes in a species-specific manner, suggesting that the diversification of pollen tube guidance molecules is one of the mechanism to prevent interspecific crosses.

Research Products

• [Journal Article] Direct interaction of ligand-receptor pairs specifying stomatal patterning (2012)
  • Author(s): Lee, JS., Kuroha, T., Hnilova, M., Khatayevich, D., Kanaoka, MM., McAbee, JM., Sarikaya, M., Tamerler, C., Torii, KU
  • Journal Title: Genes & Development Volume: 26 Pages: 126-136
  • Peer Reviewed
• [Journal Article] Expression of Arabidopsis callose synthase 5 results incallose accumulation and cell wall permeability alteration (2012)
  • Author(s): Xie, B., Deng, Y., Kanaoka, MM., Okada, K., Hong, Z.
  • Journal Title: Plant Science Volume: 183 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Expression of Arabidopsis callose synthase 5 results in callose accumulation and cell wall permeability alteration (2012)
  • Author(s): Bo Xie
  • Journal Title: Plant Science Volume: 183 Pages: 1-8
  • DOI: 10.1016/j.plantsci.2011.10.015
  • Peer Reviewed
• [Journal Article] Direct interaction of ligand-receptor pairs specifying stomatal patterning (2012)
  • Author(s): Jin-Suk Lee
  • Journal Title: Genes and Development Volume: 26 Issue: 2 Pages: 126-136
  • DOI: 10.1101/gad.179895.111
  • Peer Reviewed
• [Journal Article] Identification and characterization of TcCRP1, apollen tube attractant from Toreniaconcolor (2011)
  • Author(s): Kanaoka, MM., Kawano, N., Matsubara, Y., Susaki, D., Okuda, S., Sasaki, N., Higashiyama, T.
  • Journal Title: Annals of Botany Volume: 108 Pages: 739-747
  • Peer Reviewed
• [Journal Article] Isolation of Intact Gametophytic Protoplasts from Torenia Isolation of Intact Gametophytic Protoplasts from Torenia and Lindernia Species (2011)
  • Author(s): Kanaoka, MM.
  • Journal Title: Cytologia Volume: 76 Pages: 109-110
  • Peer Reviewed
• [Journal Article] Isolation of gametophytic cells and identification of their cell-specific markers in Torenia fournieri, T. concolorand Lindernia micrantha (2011)
  • Author(s): Kawano, N., Susaki, D., Sasaki, N., Higashiyama, T., Kanaoka, MM.
  • Journal Title: Cytologia Volume: 76 Pages: 177-184
  • Peer Reviewed
• [Journal Article] Differential effects of reproductive interference by an alien congener on native Taraxacum species (2011)
  • Author(s): Nishida, S., Takakura, K-I., Nishida, T., Matsumoto, T., Kanaoka, MM.
  • Journal Title: Biological Invasions Volume: 14 Issue: 2 Pages: 439-447
  • DOI: 10.1007/s10530-011-0088-6
  • Peer Reviewed
• [Journal Article] DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum (2011)
  • Author(s): Itoh K., Izumi A., Mori T., Dohmae N., Yui R., Maeda-Sano K., Shirai Y., Kanaoka, MM., Kuroiwa T., Higashiyama T., Sugita M., Murakami-Murofushi K., Kawano S., * Sasaki N.
  • Journal Title: Mitochondrion Volume: 11 Pages: 575-586
  • Peer Reviewed
• [Journal Article] Live-cell Imaging Reveals the Dynamics of Two Sperm Cells during Double Fertilization in Arabidopsis thaliana (2011)
  • Author(s): Hamamura Y., Saito C., Awai C., Kurihara D., Miyawaki A., Nakagawa T., Kanaoka, MM., Sasaki N., Nakano A., Berger F., * Higashiyama T.
  • Journal Title: Current Biology Volume: 21 Pages: 497-502
  • Peer Reviewed
• [Journal Article] Isolation of Gametophytic Cells and Identification of Their Cell-Specific Markers in Torenia fournieri, T. concolor and Lindernia micrantha (2011)
  • Author(s): Nao Kawano
  • Journal Title: CYTOLOGIA Volume: 76 Issue: 2 Pages: 177-184
  • DOI: 10.1508/cytologia.76.177
  • NAID: 130004448119
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed
• [Journal Article] Isolation of Intact Gametophytic Protoplasts from Torenia and Lindernia Species (2011)
  • Author(s): Masahiro Kanaoka
  • Journal Title: Cytologia Volume: 76 Pages: 109-110
  • Peer Reviewed
• [Journal Article] Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor (2011)
  • Author(s): Masahiro M.Kanaoka, 他6名
  • Journal Title: Annals of Botany Volume: 108 Issue: 4 Pages: 739-747
  • DOI: 10.1093/aob/mcr111
  • Peer Reviewed
• [Journal Article] Isolation of gametophytic cells and identification of their cell-specific markers in Torenia fournieri, T.concolor and Lindernia micrantha (2011)
  • Author(s): Nao Kawano
  • Journal Title: Cytologia Volume: 76 Pages: 177-184
  • Peer Reviewed
• [Journal Article] FERONIA as an upstream receptorkinase for polar cell growth in plants (2010)
  • Author(s): Kanaoka, MM., Torii, KU.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 107 Pages: 17461-17462
• [Journal Article] Dysregulation of cell-to-cellconnectivity and stomatal patterning byloss-of-function mutation in ArabidopsisCHORUS(GLUCAN SYNTHASE-LIKE8) (2010)
  • Author(s): Guseman, JM., Lee, JS., Bogenschutz, NL., Peterson, KM., Virata, RE., Xie, B., Kanaoka, MM., Hong, Z., * Torii, KU
  • Journal Title: Development Volume: 137 Pages: 1731-1741
  • Peer Reviewed
• [Journal Article] Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis CHORUS (GLUCAN SYNTHASE-LIKE 8) (2010)
  • Author(s): Jessica Guseman
  • Journal Title: Development 137 Pages: 1731-1741
  • Peer Reviewed
• [Journal Article] 花粉管ガイダンス物質の同定と種間多様性 (2009)
  • Author(s): 金岡雅浩、東山哲也
  • Journal Title: ブレイン・テクノニュース Volume: vol.135 Pages: 16-20
• [Journal Article] 花粉管誘引物質ルアーの発見 (2009)
  • Author(s): 東山哲也、奥田哲弘、筒井大貴、椎名恵子、武内秀憲、金岡雅浩、佐々木成江
  • Journal Title: 化学と生物 Volume: vol.47 Pages: 617-623
  • NAID: 10026436387
• [Journal Article] New Protein Pmn34 with an Exonuclease Motif Localizes in the Mitochondrial Nucleoid Periphery of Physarum polycephalum (2009)
  • Author(s): Kie Itoh
  • Journal Title: Cytologia 74 Pages: 401-407
  • Peer Reviewed
• [Journal Article] 花粉管ガイダンス物質の同定と種間多様性 (2009)
  • Author(s): 金岡雅浩
  • Journal Title: ブレインテクノニュース 135 Pages: 16-20
• [Presentation] Molecular Characterization of Pollen Tube Guidance and Its Species-specificity in Torenia (2012)
  • Author(s): 金岡雅浩
  • Organizer: 8^<th> Okazaki Biological Cconference
  • Place of Presentation: 岡崎カンファレンスホール
  • Year and Date: 2012-03-21
• [Presentation] 胚珠に由来する新規花粉管ガイダンス因子の探索 (2012)
  • Author(s): 金岡雅浩
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] トレニア近縁種における花粉管誘引物質の同定と解析 (2011)
  • Author(s): 金岡雅浩
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 花粉管誘引物質の種間多様性とその機能 (2011)
  • Author(s): 金岡雅浩
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-21
• [Presentation] 花粉管誘引物質の種間多様性とその機能 (2011)
  • Author(s): 金岡雅浩
  • Organizer: 第52回日本植物生理学会大会
  • Place of Presentation: 東北大学(震災のため大会中止)(要旨集の発行をもって発表は成立した)
  • Year and Date: 2011-03-21
• [Presentation] Molecular diversification of Cysteine-Rich Polypeptides and their functions for pollen tube guidance in Torenia (2010)
  • Author(s): 金岡雅浩
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 胚珠に由来する新規花粉管ガイダンス因子の探索 (2010)
  • Author(s): 金岡雅浩
  • Organizer: 第4回生殖研究ワークショップ
  • Place of Presentation: 筑波大学下田臨海実験所
  • Year and Date: 2010-08-20
• [Presentation] 花粉管誘引物質LUREsの種間多様性 (2010)
  • Author(s): 金岡雅浩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] 花粉管誘引物質LUREsの種間多様性 (2010)
  • Author(s): 金岡雅浩
  • Organizer: 植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20