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Identification of mammalian ovulation enzymes using in vitro ovulation system and elucidation of ovulation mechanism.

Research Project

Project/Area Number 21770067
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionHokkaido University

Principal Investigator

OGIWARA Katsueki  Hokkaido University, 大学院・理学研究院, 助教 (00422006)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords卵巣 / 排卵 / プロテアーゼ / マウス
Research Abstract

The aim of the present study were to identify the mouse ovulation enzymes responsible for degradation of the follicle wall during ovulation, and to elucidate the molecular mechanism of degradation of the follicle wall during ovulation. It was also attempted to improve the culture condition by optimizing the buffer system and by changing the supplements added. As results, it was found that two MMPs were changed their expression between before and after ovulation. However, it was suggested that they were not involved in ovulation. Active form of MT2-MMP was found to be increased before ovulation, implying that it was involved in ovulation.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes : Possible involvement in ovulation.2011

    • Author(s)
      Fujimori, C.Ogiwara, K.Hagiwara, A.Rajapakse, S. Kimura, A.Takahashi, T.
    • Journal Title

      Molecular and Cellular Endocrinology 332(1-2)

      Pages: 67-77

    • NAID

      120002834743

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes : Possible involvement in ovulation.2011

    • Author(s)
      Fujimori, C., Ogiwara, K., Hagiwara, A., Rajapakse, S., Kimura, A., Takahashi, T.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 332(1-2) Pages: 67-77

    • NAID

      120002834743

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new in vitro ovulation model for medaka based on whole ovary culture.2010

    • Author(s)
      Ogiwara, K.Ikeda, T.Takahashi, T.
    • Journal Title

      Zoolog.Sci. 27(9)

      Pages: 762-767

    • NAID

      120004094724

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression and localization of collagen type IV alchain in the medaka ovary.2010

    • Author(s)
      Kato, Y.Ogiwara, K.Fujimori, C.Kimura, A.Takahashi, T.
    • Journal Title

      Cell Tissue Research 340(3)

      Pages: 595-605

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new in vitro ovulation model for medaka based on whole ovary culture.2010

    • Author(s)
      Ogiwara, K., Ikeda, T., Takahashi, T.
    • Journal Title

      Zoological Science

      Volume: 27(9) Pages: 762-767

    • NAID

      120004094724

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and localization of collagen type IV a1 chain in the medaka ovary2010

    • Author(s)
      Kato, Y., Ogiwara, K, Fujimori, C., Kimura, A., Takahashi, T.
    • Journal Title

      Cell Tissue Research (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] メダカ排卵酵素MT2-MMPの誘導メカニズム-排卵に関与する新規チロシンキナーゼの探索と同定2010

    • Author(s)
      荻原克益
    • Organizer
      日本比較内分泌学会第35回大会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] メダカ排卵酵素MT2-MMPの排卵誘導メカニズム-排卵に関与する新規チロシンキナーゼの探索と同定2010

    • Author(s)
      荻原克益、高橋孝行
    • Organizer
      日本比較内分泌学会第35回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ排卵におけるプロスタグランジンの作用とアクチン重合への関与2010

    • Author(s)
      藤森千加、荻原克益、萩原茜、高橋孝行
    • Organizer
      日本比較内分泌学会第35回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 排卵と卵成熟は互いにコミュニケーションを取っているか?2010

    • Author(s)
      萩原茜、藤森千加、荻原克益、高橋孝行
    • Organizer
      日本比較内分泌学会第35回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] PA/プラスミン系による基底膜の分解がメダカ排卵には必要不可欠である2010

    • Author(s)
      荻原克益、高橋孝行
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 膜型プロゲステロン受容体はメダカの排卵に関与するか2010

    • Author(s)
      萩原茜、荻原克益、高橋孝行
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] EP4bがPGによるメダカ排卵の律速因子となる2010

    • Author(s)
      藤森千加、萩原茜、荻原克益、高橋孝行
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ排卵実行酵素MT2-MMPの発現誘導機構2009

    • Author(s)
      荻原克益
    • Organizer
      日本比較内分泌学会第34回大会
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] メダカの排卵とプロスタグランジン2009

    • Author(s)
      藤森千加、萩原茜、荻原克益、高橋孝行
    • Organizer
      日本動物学会北海道支部第55回大会
    • Place of Presentation
      函館
    • Year and Date
      2009-08-08
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ排卵を司る内分泌機構と排卵誘導機構の解明2009

    • Author(s)
      荻原克益、藤森千加、Sanath Rajapakse、高橋孝行
    • Organizer
      日本比較内分泌学会第34回大会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ排卵時におけるプロスタグランジン及びその受容体の解析2009

    • Author(s)
      藤森千加、萩原茜、荻原克益、高橋孝行
    • Organizer
      日本比較内分泌学会第34回大会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ卵巣におけるプロスタグランジンとその合成酵素COX-2の役割2009

    • Author(s)
      藤森千加、荻原克益、萩原茜、高橋孝行
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ卵巣特異的に発現するuPAの発現解析2009

    • Author(s)
      古舘大樹、荻原克益、高橋孝行
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス卵巣における膜結合型MMP、MT2-MMPの発現および機能解析2009

    • Author(s)
      荻原克益、高橋孝行
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      https://www.sci.hokudai.ac.jp/~kogi/Reproductive2/Welcome.html

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 魚類由来のエンテロペプチダーゼ2010

    • Inventor(s)
      高橋孝行、荻原克益
    • Industrial Property Rights Holder
      国立大学法人北海道大学
    • Acquisition Date
      2010-03-19
    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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