Identification of mammalian ovulation enzymes using in vitro ovulation system and elucidation of ovulation mechanism.
Project/Area Number |
21770067
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Morphology/Structure
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Research Institution | Hokkaido University |
Principal Investigator |
OGIWARA Katsueki Hokkaido University, 大学院・理学研究院, 助教 (00422006)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | 卵巣 / 排卵 / プロテアーゼ / マウス |
Research Abstract |
The aim of the present study were to identify the mouse ovulation enzymes responsible for degradation of the follicle wall during ovulation, and to elucidate the molecular mechanism of degradation of the follicle wall during ovulation. It was also attempted to improve the culture condition by optimizing the buffer system and by changing the supplements added. As results, it was found that two MMPs were changed their expression between before and after ovulation. However, it was suggested that they were not involved in ovulation. Active form of MT2-MMP was found to be increased before ovulation, implying that it was involved in ovulation.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes : Possible involvement in ovulation.2011
Author(s)
Fujimori, C., Ogiwara, K., Hagiwara, A., Rajapakse, S., Kimura, A., Takahashi, T.
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Journal Title
Molecular and Cellular Endocrinology
Volume: 332(1-2)
Pages: 67-77
NAID
Related Report
Peer Reviewed
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