Role of follicle-stimulating hormone on the sexual differentiation in medaka

• Project/Area Number: 21770070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Kumamoto University
• Principal Investigator: KITANO Takeshi Kumamoto University, 大学院・自然科学研究科, 准教授 (40336219)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: メダカ / 性分化 / 生殖細胞 / 濾胞刺激ホルモン

Research Abstract

To elucidate role of follicle-stimulating hormone (FSH) on the sexual differentiation in medaka, we analyzed loss-of-function of FSH receptor (FSHR) using a FSHR-defective medaka line. Moreover, we successfully established fshr-GFP transgenic medaka line by injecting a GFP-plasmid fused the regulatory region of medaka FSHR gene into the fertilized eggs.

Research Products

• [Journal Article] Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. (2010)
  • Author(s): Yamaguchi T., Yoshinaga N., Yazawa T., Gen K., Kitano T.
  • Journal Title: Endocrinology 151 Pages: 3900-3908
  • Peer Reviewed
• [Journal Article] High temperature causes masculinization of genetically female medaka by elevation of cortisol. (2010)
  • Author(s): Hayashi Y., Kobira H., Yamaguchi T., Shiraishi E., Yazawa T., Hirai T., Kamei Y., Kitano T.
  • Journal Title: Mol.Reprod.Dev. 77 Pages: 679-686
  • Peer Reviewed
• [Journal Article] Identification of a functional Medaka heat shock promoter and characterization of its ability to induce in vitro and in vivo exogenous gene expression in Medaka. (2010)
  • Author(s): Oda S., Mikami S., Urushihara Y., Murata Y., Kamei Y., Deguchi T., Kitano T., Fujimori K., Yuba S., Todo T., Mitani H.
  • Journal Title: Zool.Sci. 27 Pages: 410-415
  • Peer Reviewed
• [Journal Article] Identification of four SUMO paralogs in the medaka fish, Oryzias latipes, and their classification in two subfamilies. (2010)
  • Author(s): Seki D., Obata S., Shirozu T., Kitano T., Saitoh H.
  • Journal Title: Biochem.Genet. 48 Pages: 737-750
  • Peer Reviewed
• [Journal Article] High temperature causes masculinization of genetically female medaka by elevation of cortisol. (2010)
  • Author(s): Hayashi Y.
  • Journal Title: Molecular Reproduction and Development Volume: 77 Pages: 679-686
  • Peer Reviewed
• [Journal Article] Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. (2010)
  • Author(s): Yamaguchi T.
  • Journal Title: Endocrinology Volume: 151 Pages: 3900-3908
  • Peer Reviewed
• [Journal Article] Identification of four SUMO paralogs in the medaka fish, Oryzias latipes and their classification in two subfamilies. (2010)
  • Author(s): Seki D.
  • Journal Title: Biochemical Genetics Volume: 48 Pages: 737-750
  • Peer Reviewed
• [Journal Article] Identification of a functional Medaka heat shock promoter and characterization of its ability to induce in vitro and in vivo exogenous gene expression. (2010)
  • Author(s): Oda S.
  • Journal Title: Zoological Science
  • Peer Reviewed
• [Journal Article] Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana. (2009)
  • Author(s): Deguchi T.
  • Journal Title: Development, Growth and Differentiation 51 Pages: 769-775
  • Peer Reviewed
• [Presentation] Molecular mechanism of environmental sex determination in fish. (2010)
  • Author(s): Kitano T.(Invited)
  • Organizer: The 4th Bilateral Seminar Japan-Italy : Physical and chemical impacts on marine organisms
  • Place of Presentation: Aichi Prefectural University, Nagoya
  • Year and Date: 2010-10-25
• [Presentation] Molecular mechanism of environmental sex determination in fish. (2010)
  • Author(s): Kitano T.
  • Organizer: The 4^<th> Bilateral Seminar Japan-Italy : Physical and Chemical Impacts on Marine Organisms.
  • Place of Presentation: (Nagoya, Japan)愛知県立大学
  • Year and Date: 2010-10-25
• [Presentation] Regulational mechanism of follicle-stimulating hormone receptor expression in medaka (Oryzias latipes) (2010)
  • Author(s): Uchikawa T., Kobira H., Hirai T., Kitano T.
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] Gene expression profiling induced by high temperature treatment in medaka (Oryzias latipes) (2010)
  • Author(s): Uchimura T., Hayashi Y., Shiraishi E., Kitano T.
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] 水温コントロールによる魚類の性統御とその分子機構 (2009)
  • Author(s): 北野健
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 近畿大学農学部(奈良市)
  • Year and Date: 2009-09-11
• [Presentation] Functional analysis of follicle-stimulating hormone (FSH) and FSH-receptor in early gonadal differentiation in medaka (Oryzias latipes) (2009)
  • Author(s): Kataura H, Ichimaru H, Shiraishi E, Kanamori A, Hirai T, Kitano T.
  • Organizer: 16th International Congress of Comparative Endocrinology
  • Place of Presentation: University of Hong Kong, Hong Kong
• [Presentation] Effect of cortisol on sex differentiation in medaka (Oryzias latipes) (2009)
  • Author(s): Hayashi Y, Hisaka T, Yamada C, Kitano T.
  • Organizer: 16th International Congress of Comparative Endocrinology
  • Place of Presentation: University of Hong Kong, Hong Kong