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Cell biological analysis of GSCs/niche in medaka ovary

Research Project

Project/Area Number 21770072
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionNational Institute for Basic Biology

Principal Investigator

NAKAMURA Shuhei  National Institute for Basic Biology, 生殖遺伝学研究室, 特別協力研究員 (00510611)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords組織構築 / 幹細胞 / sox9 / GSCs / メダカ / 卵巣 / 生殖幹細 / 形態・構造 / ニッチ / 生殖幹細胞
Research Abstract

There is no direct evidence that germline stem cells and their niche are present in the vertebrate adult ovary. We identified the testis cord-like network structure, termed 'ovarian cords', which is composed of sox9b-expressing cells and germ cells of early stage oogenesis in medaka ovary. In the ovarian cord, the regions in which germ cells are clustered are present (termed 'germinal cradle'). Clonal analysis using cre/loxP system revealed that germline stem cells supplying functional eggs exist in germinal cradle (Nakamura et al., Science, 2010). Our results provide insight into germline stem cell biology of medaka and provide a model system for studying vertebrate stem cell niches.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 9 results) Presentation (5 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Ovarian germline stem cells in the teleost fish, medaka (Oryzias latipes).2011

    • Author(s)
      Nakamura, S., Kobayashi, K., Nishimura, T., Tanaka, M.
    • Journal Title

      Int J Biol Sci 7

      Pages: 403-409

    • Related Report
      2010 Final Research Report
  • [Journal Article] メダカ卵巣の配偶子幹細胞2010

    • Author(s)
      中村修平、小林佳代、西村俊哉、田中実
    • Journal Title

      細胞工学 29

      Pages: 664-669

    • Related Report
      2010 Final Research Report
  • [Journal Article] Identification of germline stem cells in the ovary of the teleost medaka.2010

    • Author(s)
      Nakamura, S., Kobayashi, K., Nishimura, T., Higashijima, S., Tanaka, M.
    • Journal Title

      Science 328

      Pages: 1561-1563

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements.2010

    • Author(s)
      Herpin, A., Braasch, I., Kraeussling, M., Schmidt, C., Thoma, E.C., Nakamura, S., Tanaka, M., Schartl, M.
    • Journal Title

      PLoS Genet 6

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of germline stem cells in the ovary of the teleost medaka2010

    • Author(s)
      Shuhei Nakamura
    • Journal Title

      Science

      Volume: 328 Pages: 1561-1563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements.2010

    • Author(s)
      Amaury Herpin
    • Journal Title

      PLoS Genetics

      Volume: 6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メダカ卵巣の配偶子幹細胞2010

    • Author(s)
      中村修平
    • Journal Title

      細胞工学

      Volume: 29 Pages: 664-669

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Expression and syntenic analyses of four nanos genes in medaka.2009

    • Author(s)
      Aoki, Y., Nakamura, S., Ishikawa, Y., Tanaka, M.
    • Journal Title

      Zool Sci 26

      Pages: 112-118

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent maintenance of cyp19a1-expressing theca cells.2009

    • Author(s)
      Nakamura, S., Kurokawa, H., Asakawa, S., Shimizu, N., Tanaka, M.
    • Journal Title

      Dev Dyn 238

      Pages: 2652-2657

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development.2009

    • Author(s)
      Herpin, A., Nakamura, S., Wagner, T.U., Tanaka, M., Schartl, M.
    • Journal Title

      Nucleic Acids Res 37

      Pages: 1510-1520

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent main tenance of cyp19a1 expressing theca cells2009

    • Author(s)
      Shuhei Nakamura
    • Journal Title

      Developmental Dynamics 238

      Pages: 2652-2657

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements2009

    • Author(s)
      Amaury Herpin
    • Journal Title

      PLoS Genetics 6

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] メダカ(Oryzias latipes)卵巣生殖幹細胞の同定2010

    • Author(s)
      中村修平、小林佳代、西村俊哉、東島眞一、田中実
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京大学・駒場キャンパス
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ(Oryzias latipes)卵巣生殖幹細胞の同定2010

    • Author(s)
      中村修平
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京大学・駒場キャンパス(東京)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sox9b function in Germline Stem Cell niche in the ovary of teleost fish, medaka2010

    • Author(s)
      Shuhei Nakamura, Ikuko Watakabe, Atsushi Toyoda, Yoshihito Taniguchi, Minoru Tanaka
    • Organizer
      Cold Spring Harbor Laboratory, Germ cell meeting
    • Place of Presentation
      Cold Spring Harbor Laboratory (New York, USA)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Identification of unique anastomosing structures containing germline stem cells in medaka ovary2009

    • Author(s)
      Shuhei Nakamura, Kayo Kobayashi, Toshiya Nishimura, Thomas Czerny, Shin-ichi Higashijima, Minoru Tanaka
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of unique anastomosing structures containing germline stem cells in medaka ovary2009

    • Author(s)
      中村修平
    • Organizer
      第32回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Book] 生物機能モデルと新しいリソース・リサーチツール(「BACやfosmidを用いたトランスジェニックメダカ作製法」)(編集:小幡裕一、城石俊彦、芹田忠夫、田中啓二、米川博通)(ISBN978-4-9000487-48-2)2011

    • Author(s)
      田中実、小林佳代、中村修平
    • Publisher
      エル・アール・シー
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.nibb.ac.jp/press/100521/100521.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/press/100521/100521.html

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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