Cell biological analysis of GSCs/niche in medaka ovary

• Project/Area Number: 21770072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: National Institute for Basic Biology
• Principal Investigator: NAKAMURA Shuhei National Institute for Basic Biology, 生殖遺伝学研究室, 特別協力研究員 (00510611)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 組織構築 / 幹細胞 / sox9 / GSCs / メダカ / 卵巣 / 生殖幹細 / 形態・構造 / ニッチ / 生殖幹細胞

Research Abstract

There is no direct evidence that germline stem cells and their niche are present in the vertebrate adult ovary. We identified the testis cord-like network structure, termed 'ovarian cords', which is composed of sox9b-expressing cells and germ cells of early stage oogenesis in medaka ovary. In the ovarian cord, the regions in which germ cells are clustered are present (termed 'germinal cradle'). Clonal analysis using cre/loxP system revealed that germline stem cells supplying functional eggs exist in germinal cradle (Nakamura et al., Science, 2010). Our results provide insight into germline stem cell biology of medaka and provide a model system for studying vertebrate stem cell niches.

Research Products

• [Journal Article] Ovarian germline stem cells in the teleost fish, medaka (Oryzias latipes). (2011)
  • Author(s): Nakamura, S., Kobayashi, K., Nishimura, T., Tanaka, M.
  • Journal Title: Int J Biol Sci 7 Pages: 403-409
• [Journal Article] メダカ卵巣の配偶子幹細胞 (2010)
  • Author(s): 中村修平、小林佳代、西村俊哉、田中実
  • Journal Title: 細胞工学 29 Pages: 664-669
• [Journal Article] Identification of germline stem cells in the ovary of the teleost medaka. (2010)
  • Author(s): Nakamura, S., Kobayashi, K., Nishimura, T., Higashijima, S., Tanaka, M.
  • Journal Title: Science 328 Pages: 1561-1563
  • Peer Reviewed
• [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements. (2010)
  • Author(s): Herpin, A., Braasch, I., Kraeussling, M., Schmidt, C., Thoma, E.C., Nakamura, S., Tanaka, M., Schartl, M.
  • Journal Title: PLoS Genet 6
  • Peer Reviewed
• [Journal Article] Identification of germline stem cells in the ovary of the teleost medaka (2010)
  • Author(s): Shuhei Nakamura
  • Journal Title: Science Volume: 328 Pages: 1561-1563
  • Peer Reviewed
• [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements. (2010)
  • Author(s): Amaury Herpin
  • Journal Title: PLoS Genetics Volume: 6
  • Peer Reviewed
• [Journal Article] メダカ卵巣の配偶子幹細胞 (2010)
  • Author(s): 中村修平
  • Journal Title: 細胞工学 Volume: 29 Pages: 664-669
• [Journal Article] Expression and syntenic analyses of four nanos genes in medaka. (2009)
  • Author(s): Aoki, Y., Nakamura, S., Ishikawa, Y., Tanaka, M.
  • Journal Title: Zool Sci 26 Pages: 112-118
  • Peer Reviewed
• [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent maintenance of cyp19a1-expressing theca cells. (2009)
  • Author(s): Nakamura, S., Kurokawa, H., Asakawa, S., Shimizu, N., Tanaka, M.
  • Journal Title: Dev Dyn 238 Pages: 2652-2657
  • Peer Reviewed
• [Journal Article] A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development. (2009)
  • Author(s): Herpin, A., Nakamura, S., Wagner, T.U., Tanaka, M., Schartl, M.
  • Journal Title: Nucleic Acids Res 37 Pages: 1510-1520
  • Peer Reviewed
• [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent main tenance of cyp19a1 expressing theca cells (2009)
  • Author(s): Shuhei Nakamura
  • Journal Title: Developmental Dynamics 238 Pages: 2652-2657
  • Peer Reviewed
• [Journal Article] Transcriptional rewiring of the sex determining dmrt1 gene duplicate by transposable elements (2009)
  • Author(s): Amaury Herpin
  • Journal Title: PLoS Genetics 6
  • Peer Reviewed
• [Presentation] メダカ(Oryzias latipes)卵巣生殖幹細胞の同定 (2010)
  • Author(s): 中村修平、小林佳代、西村俊哉、東島眞一、田中実
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京大学・駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] メダカ(Oryzias latipes)卵巣生殖幹細胞の同定 (2010)
  • Author(s): 中村修平
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京大学・駒場キャンパス(東京)
  • Year and Date: 2010-09-23
• [Presentation] Sox9b function in Germline Stem Cell niche in the ovary of teleost fish, medaka (2010)
  • Author(s): Shuhei Nakamura, Ikuko Watakabe, Atsushi Toyoda, Yoshihito Taniguchi, Minoru Tanaka
  • Organizer: Cold Spring Harbor Laboratory, Germ cell meeting
  • Place of Presentation: Cold Spring Harbor Laboratory (New York, USA)
• [Presentation] Identification of unique anastomosing structures containing germline stem cells in medaka ovary (2009)
  • Author(s): Shuhei Nakamura, Kayo Kobayashi, Toshiya Nishimura, Thomas Czerny, Shin-ichi Higashijima, Minoru Tanaka
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Identification of unique anastomosing structures containing germline stem cells in medaka ovary (2009)
  • Author(s): 中村修平
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Book] 生物機能モデルと新しいリソース・リサーチツール(「BACやfosmidを用いたトランスジェニックメダカ作製法」)(編集:小幡裕一、城石俊彦、芹田忠夫、田中啓二、米川博通)(ISBN978-4-9000487-48-2) (2011)
  • Author(s): 田中実、小林佳代、中村修平
  • Publisher: エル・アール・シー
• [Remarks] ホームページ等
  • URL: http://www.nibb.ac.jp/press/100521/100521.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/press/100521/100521.html