Structural basis of polarity protein LGN in complex with its partner molecules

• Project/Area Number: 21770116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: YUZAWA Satoru Kyushu University, 大学院・医学研究院, 学術研究員 (40515029)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 蛋白質 / シグナル伝達 / 分子認識 / X線結晶構造解析 / 細胞極性

Research Abstract

A complex in which LGN associates with a partner molecule, Insc, plays a crucial role in regulating cell polarity. To elucidate the interaction between LGN and Insc based on its tertiary structure, we have been carried out structural studies of LGN-Insc compelx using by X-ray crystallography. Regions of LGN-Insc interaction as a construct suitable for structural studies were identified and then we established the preparation of the complex. We performed crystallization trials on the LGN-Insc complex and obtained crystals suitable for X-ray diffraction after optimization of crystallization condition. X-ray diffraction data were collected to a resolution of 2.8 Å for crystals of the LGN-Insc complex.

Research Products

• [Journal Article] A conserved region between the TPR and activation domains of p67^<phox> participates in activation of the phagocyte NADPH oxidase (2010)
  • Author(s): Maehara Y., Miyano K., Yuzawa S., Akimoto R., Takeya R., Sumimoto H.
  • Journal Title: J .Biol.Chem. 285 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] A conserved region between the TPR and activation domains of p67^<phox> participates in activation of the phagocyte NADPH oxidase (2010)
  • Author(s): Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] The selectivity of receptor tyrosine kinase signaling is controlled by a secondary SH2 domain binding site. (2009)
  • Author(s): Bae, J-H., Lew, D.E., Yuzawa, S., Tome, F., Lax, I., Schlessinger, J.
  • Journal Title: Cell 138 Pages: 514-524
  • Peer Reviewed
• [Journal Article] Crystal structures of free and ligand-bound focal adhesion targeting domain of Pyk2. (2009)
  • Author(s): Lulo, J., Yuzawa, S., Schlessinger, J.
  • Journal Title: Biochem .Biophys.Res.Commun. 383 Pages: 347-352
  • Peer Reviewed
• [Journal Article] The domain organization of p67^<phox> a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. (2009)
  • Author(s): Yuzawa, S., Miyano, K., Honbou, K., Inagaki, F., Sumimoto, H.
  • Journal Title: J.Innate Immun. 1 Pages: 543-555
  • Peer Reviewed
• [Journal Article] The domain organization of p67^<phox>, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. (2009)
  • Author(s): Yuzawa, S., Miyano, K., Honbou, K., Inagaki, F., Sumimoto, H.
  • Journal Title: J.Innate Immun. 1 Pages: 543-555
  • Peer Reviewed
• [Journal Article] The selectivity of receptor tyrosine kinasc signaling is controlled by a secondary SH2 domain binding site. (2009)
  • Author(s): Bae, J-H., Lew, D.E. Yuzawa, S., Tome, F., Lax, I., Schlessinger, J.
  • Journal Title: Cell 138 Pages: 514-524
  • Peer Reviewed
• [Journal Article] Crystal structures of free and ligand-bound focal adhesion targcting domain of Pyk2. (2009)
  • Author(s): Lulo, J., Yuzawa, S., Schlessinger, J.
  • Journal Title: Biochem. Biophys. Res. Commun. 383 Pages: 347-352
  • Peer Reviewed
• [Presentation] The domain organization of p67^<phox> a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. (2009)
  • Author(s): Yuzawa, S., Miyano, K., Honbou, K., Inagaki, F., Sumimoto, H.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] The domain organization of p67^<phox>, a protein required for activation of the superoxide-producing NADPH oxidase in hagocytes (2009)
  • Author(s): Yuzawa, S., Miyano, K., Honbou, K., Inagaki, F., Sumimoto, H.
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Nox2活性化におけるp67^<phox>のドメイン配置と作用機構 (2009)
  • Author(s): 湯澤聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Nox2活性化におけるp67^<phox>のドメイン配置と作用機構 (2009)
  • Author(s): 湯沢聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
  • Organizer: 第82回 日本生化会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22