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Identification of post-translational modification in nuclear proteins during neural stem cell fate specification by proteomic analysis

Research Project

Project/Area Number 21770119
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionKumamoto University

Principal Investigator

NIIMORI Kanako  Kumamoto University, 大学院・生命科学研究部, 助教 (30457600)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords神経幹細胞 / プロテオミクス / 翻訳後修飾 / リン酸化 / 2D-DIGE / 分化制御 / 転写調節因子 / 質量分析
Research Abstract

It is not yet completely understood how neural stem cell fate is specified, in spite of extensive investigations by, for instance, gene expression profiling. To tackle this important question, we have focused on the changes in post-translational modification of nuclear proteins such as transcription factors, coactivators/corepressors, and chromatin modifiers in neural stem cells (NSCs) in response to fibroblast growth factor 2 (FGF2), a representative factor to maintain them. NSCs were stimulated by FGF2, and its lysates were examined by proteomic analysis. The result revealed 80 up- or down-regulated protein spots upon FGF2 stimulation out of 4095 spots with statistically significant differences. Among them, we focused on 24 spots differentially phosphorylated in response to FGF2, and identified all of them by nanoLC-QqTOF MS analysis. These proteins contained factors related to nuclear dynamics including nuclear translocation and chromatin modifications.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2010 2009

All Presentation (4 results)

  • [Presentation] 翻訳後修飾を指標にしたマウス神経幹細胞の分化の運命づけを司る核内分子の探索2010

    • Author(s)
      新森加納子
    • Organizer
      2010年度・第138回発生医学研究所セミナー
    • Place of Presentation
      熊本大学 発生医学研究所 招待講演
    • Year and Date
      2010-03-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 翻訳後修飾を指標にしたマウス神経幹細胞の分化の運命づけを司る核内分子の探索2009

    • Author(s)
      新森加納子, 他
    • Organizer
      神経発生討論会
    • Place of Presentation
      愛知県岡崎市(ポスター発表)
    • Year and Date
      2009-03-12
    • Related Report
      2010 Final Research Report
  • [Presentation] 翻訳後修飾を指標にしたマウス神経幹細胞の分化の運命づけを司る核内分子の探索2009

    • Author(s)
      新森加納子, 他
    • Organizer
      日本ヒトプロテオーム学会/日本ヒトプロテオーム機構第7回大会(JHUPO)
    • Place of Presentation
      東京白金北里大学(ポスター発表)
    • Related Report
      2010 Final Research Report
  • [Presentation] 翻訳後修飾を指標にしたマウス神経幹細胞の分化の運命づけを司る核内分子の探索2009

    • Author(s)
      新森加納子
    • Organizer
      日本ヒトプロテオーム機構第7回大会
    • Place of Presentation
      東京白金 北里大学
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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