Molecular Mechanisms of RAD52-Mediated Homologous Recombination
Project/Area Number |
21770127
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Waseda University |
Principal Investigator |
KAGAWA Wataru Waseda University, 理工学術院, 助教 (70415123)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 相同組換え / X線結晶構造解析 / 一本鎖DNA / タンパク質と酵素 / 核酸 / 構造生物学 / X線結晶解析 / 遺伝子及び染色体 / 分子認識 |
Research Abstract |
Homologous recombination is an important mechanism for genomic stability. To understand the underlying molecular mechanisms, three-dimensional structures of RAD52-single-stranded DNA complexes were solved by X-ray crystallography. The single-stranded DNA bound to RAD52 has a characteristic, right-handed helical structure, with bases exposed to the solvent. This structure appears suitable for the efficient search of homologous DNA sequences. The present structure provides the first clear view of how DNA homology search takes place.
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Report
(3 results)
Research Products
(55 results)