| Project/Area Number |
21770138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HIRAYAMA Jun Tokyo Medical and Dental University, 難治疾患研究所, 准教授 (90510363)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 概日リズム / 基本生理機能 / 転写 / 分子時計 / 翻訳後修飾 / 癌 / DAXX / アセチル化 / ストレス応答 / 発癌 / 生理機能 |
|---|
| Research Abstract |
Accumulating evidence suggests that the death domain-associated protein DAXX has a variety of functions in regulating apoptotic pathways. In this study, we have identified BMAL1, an essential circadian regulator, as a novel DAXX-interacting protein. The temporal association of circadian clock components is required for establishing and maintaining circadian rhythms. Notably, the interaction between endogenous DAXX and BMAL1 showed a circadian rhythmicity. Small interfering RNA (siRNA)-mediated silencing of endogenous DAXX expression and genetic ablation of the Daxx gene affected the amplitude of circadian gene expression in mammalian cells. In addition, DAXX enhanced the transcriptional capacity of the BMAL1:CLOCK heterodimer, a vital transcriptional activator in the circadian system. Our results reveal a novel function for DAXX as a modulator of circadian clock regulation.
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