Morphological plasticity and calcium dynamics in dendritic spines of neurons

• Project/Area Number: 21770161
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE Satoshi The University of Tokyo, 大学院・医学系研究科, 助教 (80302610)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脳・神経 / イメージング / ケージド化合物 / 二光子励起

Research Abstract

In hippocampal pyramidal neurons, calcium elevation causes plasticity in synaptic transmission and spine morphology. I examined calcium dynamics during induction of plasticity with repeated uncaging of caged glutamate. During stimulation that induced spine enlargement, strong calcium elevation was evoked mainly by influx through NMDA receptors, and part of the calcium in the spine diffused out to the dendritic shaft.

Research Products

• [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice. (2011)
  • Author(s): Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G.C.R., Kitamura, K., Kano, M., Matsuzaki, M., Kasai, H.
  • Journal Title: J.Physiol. 589 Pages: 2447-2457
  • Peer Reviewed
• [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice (2011)
  • Author(s): Noguchi J., et al.
  • Journal Title: Journal of Physiology Volume: (掲載確定(印刷中))
  • Peer Reviewed
• [Journal Article] Learning rules and persistence of dendritic spines. (2010)
  • Author(s): Kasai, H., Hayama, T., Ishikawa, M., Watanabe, S., Yagishita, S., Noguchi, J.
  • Journal Title: Eur.J.Neurosci. 32 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Structural dynamics of dendritic spines in memory and cognition. (2010)
  • Author(s): Kasai, H., Fukuda, M., Watanabe, S., Hayashi-Takagi, A., Noguchi, J.
  • Journal Title: Trends Neurosci. 33 Pages: 121-129
  • Peer Reviewed
• [Journal Article] Learning rules and persistence of dendritic spines (2010)
  • Author(s): Kasai H., et al
  • Journal Title: European Journal of Neuroscience Volume: 32 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Structural dynamics of dendritic spines in memory and cognition (2010)
  • Author(s): Kasai H, et al.
  • Journal Title: Trends Neurosci. 33 Pages: 121-129
  • Peer Reviewed
• [Presentation] 新しい2光子光刺激法の神経科学への応用 (2009)
  • Author(s): 河西春郎, 金本悠矢, 松崎政紀, Graham Ellis-Davis, 百武篤也, 新井達郎, Klaus Hahn, Yi Wu, 石川資子, 野口潤, 葉山達也, 渡邊恵, 森田進
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場,名古屋市
  • Year and Date: 2009-09-16
• [Presentation] 新しい2光子光刺激法の神経科学への応用 (2009)
  • Author(s): 河西春郎, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16