Analyses of the regulation of the chromatin structure and transcriptional activity using in vitro reconstitution system

• Project/Area Number: 21770167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: National Institute of Genetics (2010-2011); Kyoto University (2009)
• Principal Investigator: HIZUME Kohji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (10378846)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: タンパク質 / 核酸の構造・動態・機能 / クロマチン / ヌクレオソーム / 原子間力顕微鏡 / DNA複製 / 原子力間顕微鏡 / 試験管内再構成

Research Abstract

In order to reveal the structure induced by specific factors such as histone-tail modifications or chromosomal proteins, we observed the reconstituted chromatin under the various conditions.(1) hyper-acetylation of histone-tails causes stronger repulsive force between nucleosomes and the thinner H1-induced fiber than non-acetylated chromatin.(2) Sliding and dissociation of nucleosomes were observed by fast-AFM.(3) ORC(origin recognition complex) associated with nucleosome and formed larger complex.

Research Products

• [Journal Article] Nano-scale analyses of the chromatin decompaction induced by histone acetylation (2011)
  • Author(s): Hizume, K., Araki, S., Hata, K., Prieto, E., Kundu, T. K., Yoshikawa, K., and Takeyasu, K.
  • Journal Title: Arch Histol Cytol Volume: 73 Pages: 149-163
  • NAID: 10030516588
  • Peer Reviewed
• [Journal Article] Nano-scale analysis of the chromatin decompaction induced by histone acetylation (2011)
  • Author(s): Kohji Hizume
  • Journal Title: Archives of Histology and Cytology Volume: Vol.73,Num3 Pages: 149-163
  • Peer Reviewed
• [Journal Article] Molecular dynamics of DNA and nucleosomes in solution studied by fast-scanning atomic force microscopy (2010)
  • Author(s): Suzuki, Y., Higuchi, Y., Hizume, K., Yokokawa, M., Yoshimura, S. H., Yoshikawa, K., and Takeyasu, K.
  • Journal Title: Ultramicroscopy Volume: 110 Pages: 682-688
  • Peer Reviewed
• [Journal Article] Molecular dynamics of DNA and nucleosomes in solution studied by fast-scanning atomic force microscopy (2010)
  • Author(s): Yuki Suzuki, Yuji Higuchi, Kohji Hizume, Masatoshi Yokokawa, Shige H.Yoshimura, Kenichi Yoshikawa, Kunio Takeyasu
  • Journal Title: Ultramicroscopy (印刷中)(未定)
  • Peer Reviewed
• [Journal Article] Single-molecule anatomy by atomic force microscopy and recognition imaging (2009)
  • Author(s): Takahashi, H., Hizume, K., Kumeta, M., S, H. Y., and Takeyasu, K.
  • Journal Title: Arch Histol Cytol Volume: 72 Pages: 217-225
  • NAID: 10028159728
  • Peer Reviewed
• [Journal Article] Structural properties of yeast chromatin fiber examined by AFM and in vitro reconstitution system (2009)
  • Author(s): Prieto, E., Hizume, K., Takeyasu, K., and Yoshimura, S. H.
  • Journal Title: Proceedings of 2009 International Symposium on Micro-Nano Mechatronics and Human Science Pages: 145-149
• [Presentation] Analysis of the interaction between chromatin and the origin recognition complex using atomic force microscopy (2011)
  • Author(s): 日詰光治、矢倉勝、荒木弘之
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] Analysis of the interaction between chromatin and the origin recognition complex using atomic force microscopy (2011)
  • Author(s): 日詰光治
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Structural properties of yeast chromatin fiber examined by AFM and in vitro reconstitution system (2009)
  • Author(s): Prieto, E., Hizume, K., Takeyasu, K., and Yoshimura, S. H.
  • Organizer: 2009 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-11-10
• [Presentation] Structural properties of yeast chromatin fiber examined by AFM and in vitro reconstitution system. (2009)
  • Author(s): Kohji Hizume
  • Organizer: 2009 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-11-10