Functional analysis of PGC7/Stella as a DNA methylation regulator

• Project/Area Number: 21770207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: NAKAMURA Toshinobu Osaka University, 大学院・医学系研究科, 助教 (80403202)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: クロマチン / PGC7 / DNAメチル化 / ヒストンメチル化 / DNA脱メチル化 / PGC7/Stella / Stella

Research Abstract

We found that PGC7 protects against active DNA demethylation via the binding to di-methylated histone H3 lysine9 in fertilized egg. In addition, we found that over expression of PGC7 induced DNA demethylation in somatic cells. Furthermore, we identified Np95, which is associated with maintenance of DNA methylation, as a binding partner of PGC7. Finally, we revealed that PGC7 inhibited the binding between Np95 and replication fork.

Research Products

• [Journal Article] 初期発生の重要イベントとエピジェネティック制御 (2009)
  • Author(s): 中村肇伸、仲野徹
  • Journal Title: 再生医療 8 Pages: 23-29
• [Presentation] PGC7/Stellaによるインプリント遺伝子のDNA脱メチル化保護機構の解明 (2010)
  • Author(s): 中村肇伸、劉有容、中島啓行、立花誠、眞貝洋一、仲野徹
  • Organizer: BMB2010
  • Place of Presentation: 米子市文化ホール
  • Year and Date: 2010-12-08
• [Presentation] PGC7/Stellaによるインプリント遺伝子のDNA脱メチル化保護機構の解明 (2010)
  • Author(s): 中村肇伸、劉有容、中島啓行、立花誠、眞貝洋一、仲野徹
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Di-methylation of histone H3 lysine 9 targets PGC7/Stella to protect against active DNA demethylation in early embryogenesis (2010)
  • Author(s): Toshinobu Nakamura、Yu-Jung Liu、Hiroyuki Nakashima、Makoto Tachibana、Yoichi Shinkai、Toru Nakano
  • Organizer: International Symposium on Epigenome Network, Development and Reprogramming of Germ Cells
  • Place of Presentation: Kyushu University
  • Year and Date: 2010-11-22
• [Presentation] PGC7/Stellaによるインプリント遺伝子のDNA脱メチル化保護機構の解明 (2010)
  • Author(s): 中村肇伸、劉有容、中島啓行、立花誠、眞貝洋一、仲野徹
  • Organizer: 第4回エピジェネティック研究会
  • Place of Presentation: 米子市文化ホール
  • Year and Date: 2010-05-22
• [Presentation] PGC7/Stellaによるインプリント遺伝子のDNA脱メチル化保護機構の解明 (2010)
  • Author(s): 中村肇伸、劉有容、中島啓行、立花誠、眞貝洋一、仲野徹
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール
  • Year and Date: 2010-05-22
• [Presentation] Linkage between histone modification and DNA methylation in early embryogenesis (2010)
  • Author(s): Toshinobu Nakamura、Yu-Jung Liu、Hiroyuki Nakashima、Makoto Tachibana、Yoichi Shinkai、Toru Nakano
  • Organizer: 8th Stem Cell Research Symposium
  • Place of Presentation: Awaji Yumebutai International Conference Center
  • Year and Date: 2010-05-14
• [Presentation] Di-methylation of histone H3 lysine 9 is prerequisite for the protection against active DNA demethylation in fertilized egg (2009)
  • Author(s): Toshinobu Nakamura、Yu-Jung Liu、Hiroyuki Nakashima、Makoto Tachibana、Yoichi Shinkai、Toru Nakano
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] PGC7/StellaによるDNA脱メチル化保護機構の解明 (2009)
  • Author(s): 中村肇伸、劉有容、中島啓行、立花誠、眞貝洋一、仲野徹
  • Organizer: 第三回エピジェネティック研究会
  • Place of Presentation: 学術総合センター(東京都千代田区)
  • Year and Date: 2009-05-22
• [Book] 再生医療(初期発生の重要イベントとエピジェネティック制御)(8巻) (2009)
  • Author(s): 中村肇伸、仲野徹
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/nakano/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/nakano/