Molecular mechanism of regulation of mitosis-specific chromosome dynamics

Research Project

Project/Area Number	21770222
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Cell biology
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	KINOSHITA Kazuhisa 独立行政法人理化学研究所, 平野染色体ダイナミクス研究室, 専任研究員 (60447886)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	細胞周期 / 細胞分裂 / 染色体 / リン酸化 / キナーゼ
Research Abstract	Condensin complexes play an essential role in regulation of mitotic chromosome dynamics. We developed an experimental system to express, purify and reconstitute recombinant condensin complexes in vitro. Finally, we have succeeded in reconstituting active, recombinant holo-complexes. Using the recombinant complexes, we elucidated the contributions of the ATPase cycle and the non-SMC subunits to condensin functions.

Report

(4 results)