A live-imaging analysis of cell death dynamics and its physiological significance during mouse development

• Project/Area Number: 21770229
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAGUCHI Yoshifumi The University of Tokyo, 大学院・薬学系研究科, 助教 (10447443)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞死 / アポトーシス / カスパーゼ / ライブイメージング / 神経発生 / 発生生物学 / 神経管閉鎖

Research Abstract

Cell death is an inevitable event for multicellular organisms, but its dynamics and physiological significance in vivo remain to be elucidated. To reveal the dynamics and physiological significance of apoptosis during the neural tube closure, wherein massive apoptosis occurs, we performed a simultaneous live-imaging analysis them by using a novel transgenic mice expressing a genetically-encoded fluorescent reporter for caspase activation.

Research Products

• [Journal Article] Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system. (2010)
  • Author(s): Yoshida A, Yamaguchi Y, Nonomura K, Kawakami K, Takahashi Y, Miura M.
  • Journal Title: These authors equally contributed to this work. 15(5) Pages: 501-512
  • Peer Reviewed
• [Journal Article] Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon mediated gene transfer system. (2010)
  • Author(s): 吉田綾子、山口良文、野々村恵子、川上浩一、高橋淑子、三浦正幸
  • Journal Title: Genes to Cells Volume: 5 Pages: 501-512
  • Peer Reviewed
• [Presentation] Live-imaging analysis of apoptosis and caspase activation during neural tube closure. (2010)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Miura, M.
  • Organizer: CDB Symposium, Frontiers in Organogenesis.
  • Place of Presentation: Kobe, Japan.
• [Presentation] Live-imaging analysis of apoptosis and caspase activation reveals that apoptosis is a facilitator of the neural tube closure. (2010)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Miura, M.
  • Organizer: Society for Developmental Biology 69th Annual Meeting
  • Place of Presentation: Albuquerque, USA
• [Presentation] Apoptosis is dispensable for the control of cell number during early brain morphogenesis. (2010)
  • Author(s): Nonomura, K., Yamaguchi, Y., Yoshida, H., Miura, M.
  • Organizer: Society for Developmental Biology 69th Annual Meeting
  • Place of Presentation: Albuquerque, USA
• [Presentation] The combination of in utero electroporation technique and the Tol2 transposon system : an effective and convenient method to introduce transgenes in mammalian CNS. (2010)
  • Author(s): Yoshida A., Yamaguchi Y., Nonomura K., Kawakami K., Takahashi Y., Miura M.
  • Organizer: Gordon Research Conferences, Neural Development
  • Place of Presentation: Salve Regina Univ., USA
• [Presentation] Live-imaging analysis of apoptosis and caspase activation reveals that apoptosis is a facilitator of the neural tube closure. (2010)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Takemoto, K., Miura, M.
  • Organizer: 16^<th> International conference of the International Society of Differentiation.
  • Place of Presentation: Nara, Japan
• [Presentation] Time-lapse imaging analysis of caspase function during neural tube closure. (2010)
  • Author(s): Shinotsuka, N., Yamaguchi, Y., Takemoto, K., Miura, M.
  • Organizer: 第4回神経発生討論会
  • Place of Presentation: 岡崎
• [Presentation] Live-imaging analysis of apoptosis and caspase activation during neural tube closure by using SCAT3 transgenic mice. (2010)
  • Author(s): Shinotsuka, N., Yamaguchi, Y., Nonomura, K., Takemoto, K., Miura, M.
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都
• [Presentation] Apoptosis contributes to normal brain development not by restricting cell number but by regulating multiple morphogenetic events. (2010)
  • Author(s): Nonomura, K., Yamaguchi, Y., Yoshida, H., Miura, M.
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都
• [Presentation] Analyzing the contribution of apoptosis and caspase activation during neural tube closure by using time-lapse imaging. (2010)
  • Author(s): Shinotsuka, N., Yamaguchi, Y., Nonomuts, K., Takemoto, K., Kuida, K., Yoshida, H., Miura, M.
  • Organizer: The 16th Takeda Science Foundation Symposium on Bioscience
  • Place of Presentation: 東京
• [Presentation] Live-imaging analysis of apoptosis and caspase activation during neural tube closure. (2010)
  • Author(s): 山口良文
  • Organizer: CDB Symposium, Frontiers in Organogenesis.
  • Place of Presentation: 理研CDB(兵庫県)
• [Presentation] Live-imaging analysis of apoptosis and caspase activation reveals that apoptosis is a facilitator of the neural tube closure. (2010)
  • Author(s): 山口良文
  • Organizer: 16^<th> International conference of the International Society of Differentiation.
  • Place of Presentation: 奈良公会堂(奈良県)
• [Presentation] Live-imaging analysis of apoptosis and caspase activation during neural tube closure (2010)
  • Author(s): 山口良文
  • Organizer: CDB symposium 2010 "Organogenesis"
  • Place of Presentation: 神戸
• [Presentation] Live-imaging analysis of apoptosis and caspase activation in developing mouse brain. Cold Spring Harbor Laboratory Meeting (2009)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Miura, M.
  • Organizer: Cell Death.
  • Place of Presentation: New York, USA
• [Presentation] Caspase-mediated apoptosis is dispensable for the control of cell number in the early developing brain. (2009)
  • Author(s): Nonomura, K., Yamaguchi, Y., Yoshida, H., Miura, M.
  • Organizer: Cold Spring Harbor Laboratory Meeting, Cell Death.
  • Place of Presentation: New York, USA
• [Presentation] Live-imaging analysis of apoptosis and caspase activation in morphogenesis. (2009)
  • Author(s): Yamaguchi, Y., Shinotsuka N., Nonomura, K., Miura, M.
  • Organizer: The 4th International Workshop on Cell Regulations in Division and Arrest.
  • Place of Presentation: Okinawa, Japan
• [Presentation] Quantitative morphological analysis of mice deficient for apoptosis signaling to reveal the relationship between the change in cell number and the brain malformations. (2009)
  • Author(s): Nonomura, K., Yamaguchi, Y., Yoshida, H., Miura, M.
  • Organizer: 第3回神経発生討論会
  • Place of Presentation: 岡崎
• [Presentation] Is apoptosis required for the regulation of cell number in the early developing brain?-Quantitative analysis of morphological abnormalities in the brain of mice deficient for apoptosis signaling (2009)
  • Author(s): Nonomura, K., Yamaguchi, Y., Yoshida, H., Miura, M.
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟
• [Presentation] Utilization of the Tol2 transposon system for investigating glial development ; cell-type specific expression of transgene in glia by in utero electroporation. (2009)
  • Author(s): Yoshida, A., Yamaguchi, Y., Nonomura, K., Kawakami, K., Takahashi, Y., Miura, M.
  • Organizer: 第3回神経発生討論会
  • Place of Presentation: 岡崎
• [Presentation] Targeted expression of transgene in glial cells by the combination of the Tol2 transposon system and in utero electroporation (2009)
  • Author(s): Yoshida, A., Yamaguchi, Y., Nonomura, K., Kawakami, K., Takahashi, Y., Miura, M.
  • Organizer: 第32回日本神経科学会大会
  • Place of Presentation: 名古屋
• [Presentation] Live-imaging analysis of apoptosis in vivo by using transgenic mice expressing a genetically encoded reporter for caspase activation. (2009)
  • Author(s): Shinotsuka, N., Yamaguchi, Y., Takemoto, K., Miura, M.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Live-imaging analysis of apoptosis in vivo by using transgenic mice expressing a genetically encoded reporter for caspase activation (2009)
  • Author(s): 篠塚直美
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Live-imaging analysis of apoptosis and caspase activation in developing mouse brain (2009)
  • Author(s): 山口良文
  • Organizer: Cold Spring Harbor Laboratory Meeting, Cell Death
  • Place of Presentation: New York, USA
• [Presentation] Live-imaging analysis of apoptosis and caspase activation in morphogenesis (2009)
  • Author(s): 山口良文
  • Organizer: The 4th International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: 沖縄
• [Book] 実験医学増刊・細胞死研究総集編(三浦正幸編)(「なんのために細胞は死ぬのか?神経系発生発達期におけるプログラム細胞死の意義」) (2010)
  • Author(s): 山口良文
  • Publisher: 羊土社
• [Remarks] ホームページ等 特になし
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~genetics/team_yamaguchi.html