Molecular genetic analysis of cadmium accumulation in rice

• Project/Area Number: 21780007
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Akita Prefectural University
• Principal Investigator: NAGASAWA Namiko 秋田県立大学, 生物資源科学部・生物生産科学科, 助教 (00535289)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物育種・遺伝 / イネ / 突然変異体 / カドミウム / 育種学 / 遺伝学

Research Abstract

This research revealed how cadmium is transported from root to shoot in cadmium hyper-accumulating rice cultivar, Cho-Ko-Koku. The main gene in the mechanism was OsHMA3. OsHMA2, a homolog of OsHMA3, also functions in the pathway of cadmium transport in rice. In addition, the genetic screen was conducted in this research and several mutants were isolated.

Research Products

• [Journal Article] Mutations in rice(Oryza sativa) heavy metal ATPase 2(OsHMA2) restrict the translocation of Zn and Cd (2012)
  • Author(s): Satoh-Nagasawa N., Mori M., Nakazawa N., Kawamoto T., Nagato Y., Sakurai K., Takahashi H., Watanabe A., Akagi H.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 213-224
  • Peer Reviewed
• [Journal Article] Mutations in rice (Oryza sativa) heavy metal ATPase 2 (OsHMA2) restrict the translocation of Zn and Cd (2012)
  • Author(s): Satoh-Nagasawa N., et al
  • Journal Title: Plant Cell Physiology Volume: 53 Pages: 213-224
  • Peer Reviewed
• [Journal Article] OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles (2011)
  • Author(s): Miyadate, H., Adachi, S., Hiraizumi, A., Tezuka, K., Nakazawa, N., Kawamoto, T., Katou, K., Kodama, I., Sakurai, K., Takahashi, H., Satoh-Nagasawa, N., Watanabe, A., Fujimura, T., Akagi, H.
  • Journal Title: New Phytologist Volume: 189 Pages: 190-199
  • Peer Reviewed
• [Journal Article] OsHMA3, a P_<1B>-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles (2011)
  • Author(s): Miyadate, H., et al
  • Journal Title: New Phytologist Volume: 189 Issue: 1 Pages: 190-199
  • DOI: 10.1111/j.1469-8137.2010.03459.x
  • Peer Reviewed
• [Journal Article] A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku (2010)
  • Author(s): Tezuka, K., Miyadate, H., Katou, K., Kodama, I., Matsumoto, S., Kawamoto, T., Masaki, S., Satoh, H., Yamaguchi, M., Sakurai, K., Takahashi, H., Satoh-Nagasawa, N., Watanabe, A., Fujimura, T., Akagi, H.
  • Journal Title: Theoretical and Applied Genetics Volume: 120 Issue: 6 Pages: 1175-1182
  • DOI: 10.1007/s00122-009-1244-6
  • Peer Reviewed
• [Journal Article] A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku. (2010)
  • Author(s): Tezuka, K., Miyadate, H., Katou, K., Kodama, I., Matsumoto, S., Kawamoto, T., Masaki, S., Satoh, H., Yamaguchi, M., Sakurai, K., Takahashi, H., Satoh-Nagasawa, N., Watanabe A., Fujimura, T., Akagi, H
  • Journal Title: Theor.Appl.Genet. Volume: 120 Pages: 1175-1182
  • Peer Reviewed
• [Journal Article] Isolation of novel types of Arabidopsis mutants with altered reactions to cadmium : cadmium-gradient agar platesare an effective screen for the heavy metal-related mutants. (2010)
  • Author(s): Watanabe, A., Ito, H., Chiba, M., Ito, A., Shimizu, H., Fuji, S., Nakamura, S., Hattori, H., Chino, M., Satoh-Nagasawa, N., Takahashi, H., Sakurai, K., Akagi, H.
  • Journal Title: Planta Volume: 232 Pages: 825-836
  • Peer Reviewed
• [Journal Article] Digital gene expression signatures for maize development. (2010)
  • Author(s): Eveland, A.L., Satoh-Nagasawa, N., Goldshmidt, A., Meyer, S., Beatty, M., Sakai, H., Ware, D., Jackson, D.
  • Journal Title: Plant Physiology Volume: 154 Pages: 1024-1039
  • Peer Reviewed
• [Presentation] 水稲品種あきたこまちのモチ性変異体の解析 (2012)
  • Author(s): 堀井苑美, ら
  • Organizer: 日本育種学会2012年春季講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] ハツカダイコンGlnl;1遺伝子近傍の染色体構造が引き起こすシロイヌナズナ異数体様形態異常 (2012)
  • Author(s): 渡辺朋夫, ら
  • Organizer: 日本育種学会2012年春季講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] OsHMA2 mutants are blocked in Zn and Cd transport (2012)
  • Author(s): Satoh-Nagasawa N., Mori M., Nakazawa N., Kawamoto T., Nagato Y., Sakurai K., Takahashi H., Watanabe A., Akagi H.
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] レトロトランスポゾンTos17挿入系統を用いたイネOsHMA2遺伝子の機能解析 (2011)
  • Author(s): 佐藤(永澤)奈美子・森美果子・川本朋彦・長戸康郎・櫻井健二・高橋秀和・渡辺明夫・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] レトロトランスポゾンTos17挿入系統を用いたイネOsHMA2遺伝子の機能解析 (2011)
  • Author(s): 佐藤(永澤)奈美子, ら
  • Organizer: 日本育種学会2011年秋季講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] イネのカドミウム移行におけるP1B-type heavy-metal ATPaseの機能の解析 (2010)
  • Author(s): 森美果子・宮舘秀典・川本朋彦・櫻井健二・高橋秀和・渡辺明夫・佐藤(永澤)奈美子・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] イネのカドミウム移行におけるP_<1B>-type heavy-metal ATPaseの機能の解析 (2010)
  • Author(s): 森美果子・宮舘秀典・川本朋彦・櫻井健二・高橋秀和・渡辺明夫・佐藤(永澤)奈美子・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] イオンビームによるイネ重力屈性変異体の遺伝解析 (2010)
  • Author(s): 高橋明・手塚耕一・母良田美咲・加賀谷紘子・田中淳・佐藤(永澤)奈美子・櫻井健二・渡辺明夫・赤木宏守・高橋秀和
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] カドミウム高蓄積性イネ'長香穀'における地上部へのカドミウム移行機構 (2010)
  • Author(s): 平泉彩・安達早希・宮舘秀典・手塚耕一・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-03-27
• [Presentation] カドミウム高蓄積イネ'長香穀'の地上部へのカドミウム移行機構 (2010)
  • Author(s): 平泉彩・安達早希・宮舘秀典・手塚耕一・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会第117回講演会
  • Place of Presentation: 京都大学
• [Presentation] イネのカドミウム移行を高める遺伝子qCdT7の単離 (2009)
  • Author(s): 宮舘秀典・手塚耕一・安達早紀・平泉彩・佐藤奈美子・渡辺昭夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-25
• [Presentation] カドミウム高吸収性イネ'長香穀'のカドミウム蓄積性に関与するQTL (2009)
  • Author(s): 手塚耕一・宮舘秀典・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・佐藤秀樹・山口誠之・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-25
• [Presentation] イネのカドミウム移行を高める遺伝子qCdT7の単離 (2009)
  • Author(s): 宮舘秀典・手塚耕一・安達早希・平泉彩・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
• [Presentation] カドミウム高吸収性イネ'長香穀'のカドミウム蓄積性に関するQTL (2009)
  • Author(s): 手塚耕一・宮舘秀典・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・佐藤秀樹・山口誠之・赤木宏守
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/stic/souran/scholar/detail.php?id=224
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/stic/souran/study/detail.php?id=233
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/kyoin/kyoin0202.htm
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/kyoin/kyoin0202.htm