| Project/Area Number |
21780157
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
ISODA Keiya Forestry and Forest Products Research Institute, 林木育種センター関西育種場, 育種研究室長 (60391702)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Atsushi 独立行政法人森林総合研究所, 林木育種センター・育種第一課, 基盤技術研究室長 (10360471)
HIRAO Tomonori 独立行政法人森林総合研究所, 森林バイオ研究センター, 研究員 (90457763)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 森林生産 / 育種 / 遺伝学 / 林木育種 / eQTL / 連鎖地図 / 3'-RACE / マツノザイセンチュウ / 抵抗性 / シングルジーン / リアルタイムPCR / 遺伝子発現 / マツ / 3^'RACE / EST / SNP |
|---|
| Research Abstract |
3'-RACE was conducted using previously reported EST information of candidate resistant gene for pine wood nematode in order to determine 3'-end sequence of the ESTs. PCR primers were designed and single-genes were screened by sequencing haploid genome extracted from megagametophyto. Two primers were developed to amplify single-genes containing SNPs. Expression QTL analysis of pathogen related genes were conducted. EQTL of chitinase and PR-4 were detected in the same linkage group of phenotypic resistant trait, suggesting similar regulation factor was related to these genes and resistance
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