Studies on prolonged gestation and induction of parturition in cows carrying somatic clone fetuses

• Project/Area Number: 21780255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: 地方独立行政法人北海道立総合研究機構 (2010-2011); Hokkaido Animal Research Center (2009)
• Principal Investigator: HIRAYAMA Hiroki 地方独立行政法人北海道立総合研究機構, 農業研究本部・畜産試験場・基盤研究部, 主査 (60390861)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: クローン / 分娩 / コルチゾール / 分娩誘起 / 胎盤 / 体細胞クローン / グルコース

Research Abstract

Pre-partum abnormalities such as prolonged gestation and a lack of readiness for birth in the recipient cows cause dystocia and mortality in perinatal somatic clone calves. This study showed that plasma glucose concentration was low in cows carrying a clone fetus. In addition, mRNA abundance of glucose transporters in placentome was high in the clone pregnancy. These results raise the possibility of insufficiency in cortisol secretion from adrenal cortex of clone fetuses. The induction of parturition could not caused placental apoptosis compared with spontaneous parturition regardless of whether the fetus was derived from a nuclear transfer embryo or an in vivo fertilized embryo. Long-acting cortisol induced parturition in clone pregnancy. The findings in this study might be valuable for improving the technique of parturition induction in clone and normal pregnancies.

Research Products

• [Journal Article] Differences in apoptotic status in the bovine placentome between spontaneous and induced parturition (2012)
  • Author(s): Hirayama H, Ushizawa K, Takahashi T, Sawai K, Moriyasu S, Kageyama S, Miura R, Matsui M, Fukuda S, Naito A, Fujii T, Minamihashi A
  • Journal Title: J Reprod Dev Volume: (in press)
  • NAID: 10031123304
  • Peer Reviewed
• [Journal Article] Prepartum maternal plasma glucose concentrations and placental glucose transporter mRNA expression in cows carrying somatic cell clone fetuses (2011)
  • Author(s): Hirayama H, Sawai K, Hirayama M, Hirai T, Kageyama S, Onoe S, Minamihashi A, Moriyasu S
  • Journal Title: J Reprod Dev Volume: 57 Pages: 57-61
  • NAID: 10027901088
  • Peer Reviewed
• [Presentation] Plasma estrogen concentration and placental regulation of estrogen activity at parturition in cows carrying a somatic clone fetus (2011)
  • Author(s): 平山博樹
  • Organizer: JSPS Japan-Poland Joint Seminar Cutting-edge Reproductive Physiology-from Gamete to Baby-
  • Place of Presentation: いわて県民情報交流センター・アイーナ(招待講演)
  • Year and Date: 2011-09-14
• [Presentation] Plasma estrogen concentration and placental regulation of estrogen activity at parturition in cows carrying a somatic clone fetus (2011)
  • Author(s): Hiroki Hirayama
  • Organizer: JSPS Japan-Poland Joint Seminar Cutting-edge Reproductive Physiology. from Gamete to Baby-
  • Place of Presentation: いわて県民情報交流センター・アイーナ
• [Presentation] 体細胞クローン牛の分娩時胎盤におけるアポトーシス解析 (2010)
  • Author(s): 平山博樹
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2010-09-04
• [Presentation] 体細胞クローン牛の分娩時胎盤におけるアポトーシス解析 (2010)
  • Author(s): 平山博樹・牛澤浩一・高橋透・澤井健・森安悟・陰山聡一・尾上貞雄・南橋昭
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 北里大学
• [Presentation] 妊娠末期の体細胞クローン受胎牛における血中グルコース濃度と胎盤におけるGLUT発現 (2009)
  • Author(s): 平山博樹
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 近畿大学農学部キャンパス
  • Year and Date: 2009-09-10
• [Presentation] 妊娠末期の体細胞クローン受胎牛における血中グルコース濃度と胎盤におけるGLUT発現 (2009)
  • Author(s): 平山博樹・澤井健・陰山聡一・尾上貞雄・南橋昭・平山宗幸・平井朋和・松井義貴・森安悟
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 近畿大学