Regulation of autophagy by herpesvirus protein : the involvement in neurodegenerative disorder

• Project/Area Number: 21780260
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: TOMIOKA Yukiko 北海道大学, 遺伝子病制御研究所, 助教 (50374674)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥6,502,172 (Direct Cost: ¥5,001,671、Indirect Cost: ¥1,500,501); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,952,172 (Direct Cost: ¥1,501,671、Indirect Cost: ¥450,501); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 病態 / 疾患モデル / 発生工学 / ウイルス / 脳・神経疾患 / オートファジー / ヘルペスウイルス / 神経変性 / トランスジェニックマウス

Research Abstract

To verify that protein of herpes virus induce neurodegeneration via inhibition of cellular autophagy, the transgenic mice expressing IE180, immediate early protein of suid herpesvirus 1, were examined. P62 protein, which were degraded via selectively autophagic pathyway, and abnormal lysosome were aggregated in neuronal cells of IE180 transgenic mice. Expression of IE180 also induced accumulation of p62 in vitro. Double transgenic mice crossed IE180 transgenic mice with GFP-LC3 transgenic mice, expressing the green fluorescent protein fused to the autophagic marker LC3, were generated and examined. It seemed that IE180 transiently induced activation of autophagy, but then prevent normal autophagy and result in cell degeneration

Research Products

• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among healthy poultry flocks in farms in northern Vietnam (2012)
  • Author(s): Takakuwa, H., et al.
  • Journal Title: Preventive Veterinary Medicine Volume: 103 Issue: 2-3 Pages: 192-200
  • DOI: 10.1016/j.prevetmed.2011.09.014
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among healthy poultry flocks in farms in northern Vietnam (2011)
  • Author(s): Takakuwa, H., Yamashiro, T., Le, M. Q., Phuong, L. S., Ozaki, H., Tsunekuni, R., Usui, T., Ito, H., Morimatsu, M., Tomioka, Y., Yamaguchi, T., Ito, T., Murase, T., Ono, E., Otsuki, K.
  • Journal Title: Prev. Vet. Med Volume: 103(2-3) Pages: 192-200
  • NAID: 120006985204
  • Peer Reviewed
• [Journal Article] Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 (2011)
  • Author(s): Ochiai, K., Yoshikawa, Y., Yoshimatsu, K., Oonuma, T., Tomioka, Y., Takeda, E., Arikawa, J., Mominoki, K., Omi, T., Hashizume, K., Morimatsu, M.
  • Journal Title: FEBS Lett Volume: 585(12) Pages: 1771-1777
  • Peer Reviewed
• [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats (2011)
  • Author(s): Ochiai, K., Yoshikawa, Y., Oonuma, T., Tomioka, Y., Hashizume, K., Morimatsu, M.
  • Journal Title: Vet. J Volume: 190(2) Pages: 293-295
  • Peer Reviewed
• [Journal Article] Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 (2011)
  • Author(s): Ochiai, K., et al.
  • Journal Title: FEBS letters Volume: 585 Issue: 12 Pages: 1771-1777
  • DOI: 10.1016/j.febslet.2011.05.027
  • Peer Reviewed
• [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats (2011)
  • Author(s): Ochiai, K., et al.
  • Journal Title: The Veterinary Journal Volume: 190 Issue: 2 Pages: 293-295
  • DOI: 10.1016/j.tvjl.2010.11.001
  • Peer Reviewed
• [Journal Article] Motor-coordination-dependent learning, more than others, is impaired in transgenic mice expressing pseudorabies virus immediate-early protein IE180 (2010)
  • Author(s): Lopez-Ramos, J. C., Tomioka, Y., Morimatsu, M., Yamamoto, S., Ozaki, K., Ono, E., Delgado-Garcia, J. M.
  • Journal Title: PLoS One Volume: 5(8)
  • Peer Reviewed
• [Journal Article] Motor-coordination-dependent learning, more than others, is impaired in transgenic mice expressing pseudorabies virus immediate-early protein IE180. (2010)
  • Author(s): Lopez-Ramos.J.C
  • Journal Title: PLoS One Volume: 5(8)
  • Peer Reviewed