Establishment of substrates exploration of protozoan enzymes associated with invasion into host cells

• Project/Area Number: 21780263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The University of Tokyo
• Principal Investigator: KATO Kentaro The University of Tokyo, 大学院・農学生命科学研究科, 助教 (30401178)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 原虫 / 基質 / 酵素 / 宿主細胞侵入 / プロテインキナーゼ

Research Abstract

We have got some results that protozoa enzymes including protein kinases take parts in the important signal transductions for parasites to transfer from a stage to the next stage by analyzing the substrates and functions of enzymes relating with calcium signaling in their complicated life cycles. These results lead to the new anti-protozoan drug developments to block the protozoa-specific life cycle by inhibiting the functions of the important protozoan enzymes in the stage transfer.

Research Products

• [Journal Article] Identification of Toxonlasma cAMP dependent protein kinase and its role in the tachyzoite growth. (2011)
  • Author(s): Kurokawa H, Kato K (corresponding author), Iwanaga T, Sugi T, Sudo A, Kobayashi K, Gong H, Takemae H, Recuenco FC, Horimoto T, Akashi H.
  • Journal Title: PLoS One. In press.
  • Peer Reviewed
• [Journal Article] 1NM-PP1 treatment of mice infected with Toxonlasma (2011)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Kurokawa H, Takemae H, Gong H, Recuenco FC, Iwanaga T, Horimoto T, Akashi H.
  • Journal Title: J Vet Med Sci. In press.
  • Peer Reviewed
• [Journal Article] Equine herpesvirus 4 : Recent advances using BAC technology (2011)
  • Author(s): Azab W, Kato K, Abdel-Gawad A, Tohya Y, Akashi H.
  • Journal Title: Vet Microbiol. Volume: 150 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Bovine viral diarrhea virus nonstructural protein 5A interacts with NIK and IKKβ-binding protein (NIBP). (2010)
  • Author(s): Zahoor MA, Yamane, D, Mohamed YM, Suda Y, Kobayashi K, Kato K, Tohya T, Akashi H.
  • Journal Title: J Gen Virol. 91 Pages: 1939-1948
  • Peer Reviewed
• [Journal Article] Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxonlasma gondii CDPK1 in the invasion step. (2010)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Watanabe S, Kurokawa H, Gong H, Pandey K, Takemae H, Akashi H.
  • Journal Title: Eukaryot Cell. 9 Pages: 667-670
  • Peer Reviewed
• [Journal Article] Bovine viral diarrhea virus nonstructural protein 5A interacts with NIK and IKKβ-binding protein (NIBP) (2010)
  • Author(s): Zahoor MA, Yamane, D, Mohamed YM, Kobayashi K, Kato K, Tohya Y, Akashi H.
  • Journal Title: J Gen Virol. Volume: 91 Pages: 1939-1948
  • Peer Reviewed
• [Journal Article] Glycoprotein C of equine herpesvirus 4 plays a role in viral binding to cell surface heparan sulfate (2010)
  • Author(s): Azab W, Tsujimura K, Kato K, Kobayashi K, Mohamed YM, Maeda K, Matsumura T, Akashi H.
  • Journal Title: Virus Res. Volume: 151 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step (2010)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Watanabe S, Kurokawa H, Gong H, Pandey K, Takemae H, Akashi H.
  • Journal Title: Eukaryot Cell. Volume: 9 Pages: 667-670
  • Peer Reviewed
• [Journal Article] Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step (2010)
  • Author(s): Sugi T, Kato K(corresponding author), Kobayashi K, Watanabe S, Kurokawa H, Gong H, Pandey K, Takemae H, Akashi H.
  • Journal Title: Eukaryotic Cell. (印刷中)
  • Peer Reviewed
• [Journal Article] Characterization of a thymidine kinase-deficient mutant of equine herpesvirus 4 and in vitro susceptibility of the virus to antiviral agents (2010)
  • Author(s): Azab W, Tsujimura K, Kato K, Arii J, Morimoto T, Kawaguchi Y, Tohya Y, Matsumura T, Akashi H.
  • Journal Title: Antiviral Res. 85 Pages: 389-395
  • Peer Reviewed
• [Journal Article] Molecular analyses of Toxonlasma calmodulin-like domain protein kinase isoform 3. (2009)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Pandey K, Takemae H, Kurokawa H, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 416-423
  • Peer Reviewed
• [Journal Article] Characterization of plasmodium falciparum calcium-dependent protein kinase 4. (2009)
  • Author(s): Kato K (corresponding author), Sudo A, Kobayashi K, Sugi T, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 394-400
  • NAID: 10027670595
  • Peer Reviewed
• [Journal Article] Molecular analyses of Toxoplasma gondii calmodulin-like domain protein kinase isoform 3 (2009)
  • Author(s): Sugi T, Kato K(corresponding author), Kobayashi K, Pandey K, Takemae H, Kurokawa H, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 416-423
  • NAID: 10027670734
  • Peer Reviewed
• [Journal Article] Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 (2009)
  • Author(s): Kato K(corresponding author), Sudo A, Kobayashi K, Sugi T, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 394-400
  • NAID: 10027670595
  • Peer Reviewed
• [Journal Article] Activation of extracellular signal-regulated kinase in MDBK cells infected with bovine viral diarrhea virus (2009)
  • Author(s): Yamane D, Zahoor MA, Mohamed YM, Azab W, Kato K, Tohya Y, Akashi H.
  • Journal Title: Arch Virol 154 Pages: 1499-1503
  • Peer Reviewed
• [Journal Article] Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis (2009)
  • Author(s): Yamane D, Zahoor MA, Mohamed YM, Azab W, Kato K, Tohya Y, Akashi H.
  • Journal Title: J Biol Chem. 284 Pages: 13648-13659
  • Peer Reviewed
• [Presentation] トキソプラズマ原虫の新規プロテインキナーゼの機能解析 (2010)
  • Author(s): 加藤健太郎、小林郷介、杉達紀、竹前等、ゴン海燕、黒川瞳、明石博臣
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広畜産大学(北海道)
  • Year and Date: 2010-09-18
• [Presentation] トキソプラズマ原虫のcAMP依存性プロテインキナーゼの機能解析 (2010)
  • Author(s): 黒川瞳、加藤健太郎、首藤篤史、小林郷介、杉達紀、竹前等、ゴン海燕、明石博臣
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広畜産大学(北海道)
  • Year and Date: 2010-09-18
• [Presentation] ASKA法を用いたトキソプラズマ原虫CDPK1薬剤標的妥当性評価 (2010)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、竹前等、ゴン海燕、黒川瞳、レクエンコ・フランセス、岩永達也、堀本泰介、明石博臣
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広畜産大学(北海道)
  • Year and Date: 2010-09-18
• [Presentation] Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 (2010)
  • Author(s): Kentaro Kato, Atsushi Sudo, Kyousuke Kobayashi, Tatsuki Sugi, Yukinobu Tohya, Hiroomi Akashi
  • Organizer: XIIth International Congress of Parasitology (ICOPAXII)
  • Place of Presentation: メルボルン(オーストラリア)
  • Year and Date: 2010-08-17
• [Presentation] トキソプラズマ原虫におけるcAMP依存性プロテインキナーゼの機能解析 (2010)
  • Author(s): 黒川瞳、加藤健太郎、首藤篤史、小林郷介、杉達紀、竹前等、ゴン海燕、堀本泰介、明石博臣
  • Organizer: 第18回分子寄生虫ワークショップ
  • Place of Presentation: 草津セミナーハウス(群馬県)
  • Year and Date: 2010-08-02
• [Presentation] ASKA法によって明らかにされた、トキソプラズマ原虫CDPK1による滑走運動制御 (2010)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、明石博臣
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道)
  • Year and Date: 2010-05-20
• [Presentation] マラリア原虫独自酵素によるその巧妙なライフサイクルコントロール (2010)
  • Author(s): 加藤健太郎
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-27
• [Presentation] Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 (2010)
  • Author(s): Kentaro Kato, Atsushi Sudo, Kyosuke Kobayashi, Tatsuki Sugi, Yukinobu Tohya, Hiroomi Akashi.
  • Organizer: XIIth International Congress of Parasitology (ICOPAXII)
  • Place of Presentation: Melbourne, Australia
• [Presentation] トキソプラズマ原虫におけるcAMP依存性プロテインキナーゼの機能解析 (2010)
  • Author(s): 黒川瞳、加藤健太郎、首藤篤史、小林郷介、杉達紀、竹前等、ゴン海燕、堀本泰介、明石博臣
  • Organizer: 第18回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
• [Presentation] ASKA法によって明らかにされた、トキソプラズマ原虫CDPK1による滑走運動制御 (2010)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、明石博臣
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 北海道
• [Presentation] マラリア原虫独自酵素によるその巧妙なライフサイクルコントロール (2010)
  • Author(s): 加藤健太郎
  • Organizer: 第149回日本獣医学会,第1回獣医寄生虫学奨励賞受賞
  • Place of Presentation: 東京
• [Presentation] トキソプラズマ原虫CDPKの原虫生活環における役割 (2009)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、遠矢幸伸、明石博臣
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
• [Presentation] マラリア原虫のPfCDPK4の有性生殖期における発現解析 (2009)
  • Author(s): 加藤健太郎、小林郷介、杉達紀、遠矢幸伸、明石博臣
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
• [Presentation] アピコンプレクサ門原虫プロテインキナーゼ特異的阻害剤探索 (2009)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、黒川瞳、明石博臣
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
• [Presentation] 牛ウイルス性下痢ウイルスによるスフィンゴ脂質代謝制御を介したウイルス複製機構の解析 (2009)
  • Author(s): 山根大典、加藤健太郎、遠矢幸伸、明石博臣
  • Organizer: 第147回日本獣医学会
  • Place of Presentation: 栃木
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/kato100113.html
• [Patent(Industrial Property Rights)] Toxoplasma gondii RON4-binding molecules by retrovirus-mediated expression cloning (2011)
  • Inventor(s): Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi, Hitoshi Takemae
  • Industrial Property Rights Holder: 東京大学(予定)
  • Filing Date: 2011-02-28
  • Overseas
• [Patent(Industrial Property Rights)] Toxoplasma gondii RON4-binding molecules by retrovirus-mediated expression cloning (2010)
  • Inventor(s): Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi, Hitoshi Takemae
  • Industrial Property Rights Holder: Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi, Hitoshi Takemae
  • Filing Date: 2010-02-28
• [Patent(Industrial Property Rights)] 原虫プロテインキナーゼの即時的機能抑制が可能なトキソプラズマ原虫株 (2009)
  • Inventor(s): 加藤健太郎、明石博臣、杉達紀
  • Industrial Property Rights Holder: 加藤健太郎
  • Industrial Property Number: 2009-286975
  • Filing Date: 2009-12-17