| Project/Area Number |
21780270
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OGUSHI Sugako The Institute of Physical and Chemical Research, 生殖系列研究チーム, 基礎科学特別研究員 (50437505)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 卵母細胞 / 核小体 / 初期胚発生 / ヌクレオプラスミン2(Npm2) / ヌクレオプラスミン / 哺乳類 |
|---|
| Research Abstract |
In mammals, the oocyte nucleolus is required for early embryonic development, but the function of oocyte nucleolus is elusive. Detailed analysis revealed that the zygotes lacking nucleolus showed the delay of sperm chromatin decondensation, constitutive heterochromatin disorganization during interphase, and the defect in first mitotic progression. These results indicate that the oocyte nucleolus is involved in the functional chromatin organization in zygotes. Moreover, we identified the proteins interacted with nucleoplasmin2 (Npm2) in the oocyte nucleolus. The function of these proteins is under analysis.
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