Obstruction of HA antigen cleavage of influenza virus with recombinant H chain antibody and its antiviral effects

• Project/Area Number: 21780280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Kansai Medical University
• Principal Investigator: LEE Sung-Il Kansai Medical University, 医学部, 准教授 (90361964)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: インフルエンザウイルス / H鎖抗体 / 人獣共通感染症

Research Abstract

It is a research that develops H chain antibody of the camel family animal, reported to have an excellent antigen specificity and compatibility, to control influenza virus HA protein effectively, and that provide the treatment model of the virus disease. The H chain antibodies were separated from the llama immunized with HA antigen, and were reactive to the HA antigen after the treat for 30 minutes at 90℃. The amplified VHH antibody gene PCR products were introduced into phagemid vectors and separated from transformed DH5α. It is necessary to examine the utility of the gene restructuring, by an artificial mutation introduction or the DNaseI processing, to isolate the VHH clone with a stronger affinity.

Research Products

• [Journal Article] Immunoglobulin profiles in acute brucellosis experimentally induced by Brucella canis in BALB/c mice. (2010)
  • Author(s): Sung-Il Lee, Md.Ariful Islam, Mst.Minara Khatun, Gyu-Young Choi, Jae-Myeong Jung, Byeong-Kirl Baek, Ibulaimu Kakoma
  • Journal Title: Vector Borne Zoonotic Diseases 10 Pages: 927-930
  • Peer Reviewed
• [Journal Article] Immunoglobulin profiles in acute brucellosis experimentally induced by Brucella canis in BALB/c mice. (2010)
  • Author(s): Lee, S.-I., Islam, M.A., Khatun, M.M., Choi, G.Y., Jung, J.M., Baek, B.-K., Kakoma, I.
  • Journal Title: Vector Borne Zoonotic Diseases Volume: 10 Pages: 927-930
  • Peer Reviewed
• [Journal Article] Construction of artificial promoters senistivity responsive to sonication in vitro. (2009)
  • Author(s): Akihiko Watanabe, Satoshi Kakutani, Ryohei Ogawa, Sung-il Lee, Toru Yoshida, Akihiro Morii, Go Kagiya, Loreto B.Feril Jr., Hideki Fuse, Takashi Kondo
  • Journal Title: Journal of Medical Ultrasonics 36 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Enhancement of artificial promoter activity by ultrasound-induced oxidative stress. (2009)
  • Author(s): Ryohei Ogawa, Sung-il Lee, Hironori Izumi, Go Kagiya, Toru Yohsida, Akihiko Watanabe, Akihiro Morii, Satoshi Kakutani, Takashi Kondo, Loreto B.Feril Jr., Tetsuya Ishimoto
  • Journal Title: Ultrasonic Sonochemistry 16 Pages: 379-386
  • Peer Reviewed
• [Journal Article] Cellular and humoral immune responses and antigen recognition in Sprague-Dawleyrats experimentally infected with Brucella abortus biotype 1. (2009)
  • Author(s): Mst.Minara Khatun, Md.Ariful Islam, Byeong-kirl Baek, Sung-il Lee
  • Journal Title: Asian Journal of Animal and Veterinary Advances 4 Pages: 267-277
  • Peer Reviewed
• [Journal Article] Characteristics of the immune response during acute brucellosis in Sprague-Dawley rats. (2009)
  • Author(s): Mst.Minara Khatun, Md.Ariful Islam, Byeong-kirl Baek, Sung-il Lee
  • Journal Title: Journal of Infection in Developing Countries 3 Pages: 392-397
  • Peer Reviewed
• [Journal Article] Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats. (2009)
  • Author(s): Md.Ariful Islam, Mst.Minara Khatun, Byeong-kirl Baek, Sung-il Lee
  • Journal Title: Journal of Veterinary Science 10 Pages: 211-218
  • Peer Reviewed
• [Journal Article] Analysis of human brucellosis sera using western blot assay. (2009)
  • Author(s): Sung-il Lee, Min-jun Choi, Mst.Minara Khatun, Md.Ariful Islam, Byeong-kirl Baek, Chang-Seup Lee, Ibulaimu Kakoma.
  • Journal Title: Korean Journal of Veterinary Public Health 33 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Enhancement of artificial promoter activity by ultrasound-induced oxidative stress (2009)
  • Author(s): Ogawa, R., Lee, S.-I., Izumi, H., Kagiya, G., Yoshida, T., Watanabe, A., Morii, A., Kakutani, S., Kondo, T., Feril, L.B., Ishimoto, T.
  • Journal Title: Ultrasonics Sonochemistry 16 Pages: 379-386
  • Peer Reviewed
• [Journal Article] Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats (2009)
  • Author(s): Islam, M.A., Khatun, M.M., Baek, B.-K., Lee, S.-I.
  • Journal Title: Journal of Veterinary Science 10 Pages: 211-218
  • Peer Reviewed
• [Journal Article] Cellular and humoral immune responses and antigen recognition in Sprague-Dawley rats experimentally infected with Brucella abortus biotype 1 (2009)
  • Author(s): Khatun, M.M., Islam, M.A., Baek, B.-K., Lee, S.-I.
  • Journal Title: Asian Journal of Animal and Veterinary Advances 4 Pages: 267-277
  • Peer Reviewed