Development of a sensitive method for catecholamines using on-chip liquid chromatography

• Project/Area Number: 21790033
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: TSUNODA Makoto 東京大学, 大学院・薬学系研究科, 講師 (10323453)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分析化学 / クロマトグラフィー / マイクロ化学チップ / 生体分子 / マイクロ・ナノデバイス

Research Abstract

The separation efficiency of a pillar array column under pressure-driven liquid chromatography conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. Two coumarin dyes were well resolved under reversed-phase conditions with a maximum theoretical plate number of 8000. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s.

Research Products

• [Journal Article] Use of folded micromachined pillar array column with low-dispersion turns for pressure-driven liquid chromatography (2010)
  • Author(s): C. Aoyama, A. Saeki, M. Noguchi, Y. Shirasaki, S. Shoji, T. Funatsu, J. Mizuno, M. Tsunoda
  • Journal Title: Anal. Chem. Volume: 82 Pages: 1420-1426
• [Journal Article] Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography (2010)
  • Author(s): M. Tsunoda, C. Aoyama, H. Nomura, T. Toyoda, N. Matsuki, T. Funatsu
  • Journal Title: J. Pharm. Biomed. Anal. Volume: 51 Pages: 712-715
• [Journal Article] Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography (2010)
  • Author(s): 角田誠, ら
  • Journal Title: J.Pharm.Biomed.Anal. Volume: 51 Pages: 712-715
  • Peer Reviewed
• [Journal Article] Use of folded micromachined pillar array column with low-dispersion turns for pressure-driven liquid chromatography (2010)
  • Author(s): 青山千顕, ら
  • Journal Title: Analytical Chemistry 82 Pages: 1420-1426
  • Peer Reviewed
• [Presentation] オンチップ分離技術の開発 (2010)
  • Author(s): 角田誠
  • Organizer: 岡山大&理研ジョイントシンポジウム
  • Year and Date: 2010-06-28
• [Presentation] MEMS FABRICATED LIQUID CHROMATOGRAPHY MICROCHIP FOR PRACTICAL USES (2010)
  • Author(s): Masao Noguchi, Makoto Tsunoda, Jun Mizuno, Takashi Funatsu, Shuichi Shoji
  • Organizer: The 23rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2010)
  • Year and Date: 2010-01-27
• [Presentation] MEMS FABRICATED LIQUID CHROMATOGRAPHY MICROCHIP FOR PRACTICAL USES (2010)
  • Author(s): Masao Noguchi
  • Organizer: The 23rd IEEE International Conference on Micro Electro Mechanical Systems(MEMS 2010)
  • Place of Presentation: Hong Kong China
  • Year and Date: 2010-01-27