| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
In this study, a highly sensitive immunoassay was developed by using DNA amplification technique as detection system. Under isothermal condition, LAMP(Loop-mediated isothermal Amplification) and RCA(Rolling Circle Amplification) reaction enable successive DNA amplification. In order to apply DNA amplification to the detection system on immunoassay, DNA-conjugated antigen was prepared for competitive immunoassay. As a result, physically and chemically stable DNA provided highly efficient and sensitive signal amplification based on genetic engineering.
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