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Development of discovering technology to quantify the biomarker for minimally invasive diagnostics.

Research Project

Project/Area Number 21790173
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Medical pharmacy
Research InstitutionNational Institute of Biomedical Innovation

Principal Investigator

鎌田 春彦  National Institute of Biomedical Innovation, 創薬基盤研究部, サブプロジェクトリーダー (00324509)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバイオマーカー / プロテオーム解析 / 膜蛋白質 / ビオチンラベル化 / 肝炎
Research Abstract

The proteins they express at the time of disease and which are related to organ dysfunction are poorly characterized. Our group has developed a general chemical proteomics approach for the identification of biomarker proteins. The aim of this study was to identify protein biomarkers present in sinusoidal endothelial cells following hepatic injury using chemical proteomics employing in vivo biotinylation. We performed a proteomic study in a mouse model of massive hepatocyte apoptosis in tumor necrosis factor (TNF)/D-(+)-galactosamine (GalN) animals induced by the combined administration of GalN and TNF. In vivo biotinylation was carried out by perfusing mice with a reactive ester derivative of biotin that enables the covalent modification of proteins. Protein expression was analyzed using purified proteins from hepatitic and normal livers. The biotinylated proteins were purified from liver extracts using streptavidin beads, digested on the resin by trypsin and subjected to mass spectrometry for identification. These results indicate that the sinusoidal endothelial biomarkers expressed on liver injury reflect the molecular pathobiology and the mechanisms of hepatitis induction.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (57 results)

All 2011 2010 2009 Other

All Journal Article (46 results) (of which Peer Reviewed: 23 results) Presentation (8 results) Remarks (3 results)

  • [Journal Article] Creation of an improved mutant TNF with TNFR1-selectivity and antagonistic activity by phage display technology.2010

    • Author(s)
      Nomura T., Abe Y., Kamada H., Inoue M., Kawara T., Arita S., Furuya T., Minowa K., Yoshioka Y., Shibata H., Kayamuro H., Yamashita T., Nagano K., Yoshikawa T., Mukai Y., Nakagawa S., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 65(2)

      Pages: 93-96

    • Related Report
      2010 Final Research Report
  • [Journal Article] Generation of mouse macrophages expressing membrane-bound TNF variants with selectivity for TNFR1- or TNFR2.2010

    • Author(s)
      Shibata H., Abe Y., Yoshioka Y., Nomura T., Sato M., Kayamuro H., Kawara T., Arita S., Furuya T., Nagano K., Yoshikawa T., Kamada H., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Cytokine 50(1)

      Pages: 75-83

    • Related Report
      2010 Final Research Report
  • [Journal Article] An in vitro method for screening anti-platelet agents using a microchannel array flow analyzer.2010

    • Author(s)
      Kamada H., Okamoto T., Hayashi T., Suzuki K.
    • Journal Title

      Biorheology 47(2)

      Pages: 153-161

    • Related Report
      2010 Final Research Report
  • [Journal Article] Size-dependent cytotoxic effects of amorphous silica nanoparticles on Langerhans cells.2010

    • Author(s)
      Nabeshi H., Yoshikawa T., Matsuyama K., Nakazato Y., Arimori A., Isobe M., Tochigi S., Kondoh S., Hirai T., Akase T., Yamashita T., Yamashita K., Yoshida T., Nagano K., Abe Y., Yoshioka Y., Kamada H., Imazawa T., Itoh N., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 65(3)

      Pages: 199-201

    • Related Report
      2010 Final Research Report
  • [Journal Article] Mutant TNF-alpha, mTNF-K90R, is a novel mucosal vaccine adjuvant candidate against HIV.2010

    • Author(s)
      Kayamuro H., Abe Y., Yoshioka Y., Katayama K., Yoshida T., Yamashita K., Yoshikawa T., Kawai Y., Mayumi T., Hiroi T., Itoh N., Nagano K., Kamada H., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 65(4)

      Pages: 254-6

    • Related Report
      2010 Final Research Report
  • [Journal Article] Carbon nanotubes elicit DNA damage and inflammatory response relative to their size and shape.2010

    • Author(s)
      Yamashita K., Yoshioka Y., Higashisaka K., Morishita Y., Yoshida T., Fujimura M., Kayamuro H., Nabeshi H., Yamashita T., Nagano K., Abe Y., Kamada H., Kawai Y., Mayumi T., Yoshikawa T., Itoh N., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Inflammation 33(4)

      Pages: 276-80

    • Related Report
      2010 Final Research Report
  • [Journal Article] Urban aerosol induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo.2010

    • Author(s)
      Yoshida T., Yoshioka Y., Fujimura M., Kayamuro H., Yamashita K., Higashisaka K., Nakanishi R., Morishita Y., Nabeshi H., Yamashita T., Muroi M., Tanamoto K., Nagano K., Abe Y., Kamada H., Kawai Y., Mayumi T., Itoh N., Yoshikawa T., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biol.Pharm.Bull. 33(5)

      Pages: 780-3

    • NAID

      130000248036

    • Related Report
      2010 Final Research Report
  • [Journal Article] Arsenic trioxide alters expression and oxidative modification of the proteome in leukemic cells.2010

    • Author(s)
      Nabeshi H., Yoshikawa T., Kamada H., Shibata H., Sugita T., Abe Y., Nagano K., Nomura T., Minowa K., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 65

      Pages: 702-707

    • Related Report
      2010 Final Research Report
  • [Journal Article] The IL-1 family cytokines as mucosal vaccine adjuvant for the induction of protective immunity against influenza virus.2010

    • Author(s)
      Kayamuro H., Yoshioka Y., Abe Y., Arita S., Katayama K., Nomura T., Yoshikawa T., Kubota-Koketsu R., Ikuta K., Okamoto S., Mori Y., Kunisawa J., Kiyono H., Itoh N., Nagano K., Kamada H., Tsutsumi Y., Tsunoda S.
    • Journal Title

      J.Virol. 84(24)

      Pages: 12703-12712

    • Related Report
      2010 Final Research Report
  • [Journal Article] Potential adjuvant effect of intranasal urban aerosols in mice through induction of dendritic cell maturation.2010

    • Author(s)
      Yoshida T., Yoshioka Y., Fujimura M., Yamashita K., Higashisaka K., Nakanishi R., Morishita Y., Kayamuro H., Nabeshi H., Nagano K., Abe Y., Kamada H., Tsunoda S., Yoshikawa T., Itoh N., Tsutsumi Y.
    • Journal Title

      Toxicol.Lett. 199(3)

      Pages: 383-388

    • Related Report
      2010 Final Research Report
  • [Journal Article] In vivo biotinylation of the vasculature in B cell lymphoma identifies BST-2 as a target for antibody-based therapy.2010

    • Author(s)
      Schliemann C., Roesli C., Kamada H., Borgia B., Fugmann T., Klapper W., Neri D.
    • Journal Title

      Blood 115(3)

      Pages: 736-744

    • Related Report
      2010 Final Research Report
  • [Journal Article] An in vitro method for screening anti-platelet agents using a microchannel array flow analyzer.2010

    • Author(s)
      Kamada H., et al.
    • Journal Title

      Biorheology

      Volume: 47 Pages: 156-161

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo biotinylation of the vasculature in B cell lymphoma identifies BST-2 as a target for antibody-based therapy.2010

    • Author(s)
      Schliemann C., Kamada H., et al.
    • Journal Title

      Blood

      Volume: 115 Pages: 736-744

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic trioxide induces down-regulation of gp46 via protein oxidation : proteomics analysis of oxidative modified proteins in As2O3-treated HTLV-1-infected cells.2010

    • Author(s)
      Nabeshi H., Kamada H., et al.
    • Journal Title

      Pharmazie

      Volume: 65 Pages: 702-707

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Creation of an improved mutant TNF with TNFR1-selectivity and antagonistic activity by phage display technology.2010

    • Author(s)
      Nomura T., Kamada H., et al.
    • Journal Title

      Pharmazie

      Volume: 65 Pages: 93-96

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of mouse macrophages expressing membrane-bound TNF variants with selectivity for TNFR1- or TNFR22010

    • Author(s)
      Shibata H., Kamada H., et al.
    • Journal Title

      Cytokine

      Volume: 50 Pages: 75-83

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Size-dependent cytotoxic effects of amorphous silica nanoparticles on Langerhans cells.2010

    • Author(s)
      Nabeshi H., Kamada H., et al.
    • Journal Title

      Pharmazie

      Volume: 65 Pages: 199-201

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutant TNF-alpha, mTNF-K90R, is a novel mucosal vaccine adjuvant candidate against HIV.2010

    • Author(s)
      Kayamuro H., Kamada H., et al.
    • Journal Title

      Pharmazie

      Volume: 65 Pages: 254-256

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carbon nanotubes elicit DNA damage and inflammatory response relative to their size and shape.2010

    • Author(s)
      Yamashita K., Kamada H., et al.
    • Journal Title

      Inflammation

      Volume: 33 Pages: 276-280

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Urban aerosol induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo.2010

    • Author(s)
      Yoshida T., Kamada H., et al.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 33 Pages: 780-783

    • NAID

      130000248036

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The IL-1 family cytokines as mucosal vaccine adjuvant for the induction of protective immunity against influenza virus.2010

    • Author(s)
      Kayamuro H, Kamada H., et al.
    • Journal Title

      J.Virol

      Volume: 84 Pages: 12703-12712

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential adjuvant effect of intranasal urban aerosols in mice through induction of dendritic cell maturation.2010

    • Author(s)
      Yoshida T., Kamada H., et al.
    • Journal Title

      Toxicol.Lett.

      Volume: 199 Pages: 383-388

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function relationship of tumor necrosis factor (TNF) and its receptor interaction based on 3D structural analysis of a fully active TNFR1-selective TNF mutant.2009

    • Author(s)
      Mukai Y., Shibata H., Nakamura T., Yoshioka Y., Abe Y., Nomura T., Taniai M., Ohta T., Ikemizu S., Nakagawa S., Tsunoda S., Kamada H., Yamagata Y., Tsutsumi Y.
    • Journal Title

      J.Mol.Biol. 385

      Pages: 1221-1229

    • Related Report
      2010 Final Research Report
  • [Journal Article] The specific effect of 2-Methoxyestradiol on lymphatic vascular endothelial cells.2009

    • Author(s)
      Imai S., Yoshida Y., Okamura T., Nagano K., Abe Y., Yoshikawa T., Kamada H., Nakagawa S., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 64(3)

      Pages: 214-6

    • Related Report
      2010 Final Research Report
  • [Journal Article] Rapid isolation of intrabody candidates by using an optimized non-immune phage antibody library.2009

    • Author(s)
      Nagano K., Imai S., Mukai Y., Nakagawa S., Abe Y., Kamada H., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Pharmazie 64(4)

      Pages: 238-41

    • Related Report
      2010 Final Research Report
  • [Journal Article] Crystallization and preliminary X-ray analysis of TNF-TNFR2 complex, Acta. Crystallogr.2009

    • Author(s)
      Mukai Y., Nakamura T., Yoshioka Y., Tsunoda S., Kamada H., Nakagawa S., Yamagata Y., Tsutsumi Y.
    • Journal Title

      Sect.F. 65(Pt3)

      Pages: 295-8

    • Related Report
      2010 Final Research Report
  • [Journal Article] The augmentation of intracellular delivery of peptide therapeutics by artificial protein transduction domains.2009

    • Author(s)
      Yoshikawa T., Sugita T., Mukai Y., Yamanada N., Nagano K., Nabeshi H., Shibata H., Yoshioka Y., Nakagawa S., Kamada H., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biomaterials. 30(19)

      Pages: 3318-23

    • Related Report
      2010 Final Research Report
  • [Journal Article] Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF : useful information for designing artificial proteo-antagonists.2009

    • Author(s)
      Mukai Y., Nakamura T., Yoshioka Y., Shibata H., Abe Y., Nomura T., Taniai M., Ohta T., Nakagawa S., Tsunoda S., Kamada H., Yamagata Y., Tsutsumi Y.
    • Journal Title

      J.Biochem. 146(2)

      Pages: 167-72

    • NAID

      10026302534

    • Related Report
      2010 Final Research Report
  • [Journal Article] TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.2009

    • Author(s)
      Kayamuro H., Yoshioka Y., Abe Y., Katayama K., Yoshida T., Yamashita K., Yoshikawa T., Hiroi T., Itoh N., Kawai Y., Mayumi T., Kamada H., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 384(3)

      Pages: 296-300

    • Related Report
      2010 Final Research Report
  • [Journal Article] Arsenic trioxide has the inhibitory effect on transmission of human T-cell leukemia virus type 1.2009

    • Author(s)
      Nabeshi H., Yoshikawa T., Kamada H., Shibata H., Sugita T., Abe Y., Nagano K., Nomura T., Minowa K., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biol.Pharm.Bull. 32(7)

      Pages: 1286-8

    • Related Report
      2010 Final Research Report
  • [Journal Article] The use of a mutant TNF-alpha as a vaccine adjuvant for the induction of mucosal immune responses.2009

    • Author(s)
      Kayamuro H., Abe Y., Yoshioka Y., Katayama K., Yoshida T., Yamashita K., Yoshikawa T., Hiroi T., Itoh N., Kawai Y., Kamada H., Nagano K., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biomaterials 30(29)

      Pages: 5869-5876

    • Related Report
      2010 Final Research Report
  • [Journal Article] Novel protein engineering strategy for creating highly receptor-selective mutant TNFs.2009

    • Author(s)
      Nomura T., Abe Y., Kamada H., Inoue M., Kawara T., Arita S., Furuya T., Yoshioka Y., Shibata H., Kayamuro H., Yamashita T., Nagano K., Yoshikawa T., Mukai Y., Nakagawa S., Taniai M., Ohta T., Tsunoda S., Tsutsumi Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 30;388(4)

      Pages: 667-71

    • Related Report
      2010 Final Research Report
  • [Journal Article] The treatment of established murine collagen-induced arthritis with a TNFR1-selective antagonistic mutant TNF.2009

    • Author(s)
      Shibata H., Yoshioka Y., Abe Y., Ohkawa A., Nomura T., Minowa K., Mukai Y., Nakagawa S., Taniai M., Ohta T., Tsunoda S., Kamada H., Tsutsumi Y.
    • Journal Title

      Biomaterials. 30(34)

      Pages: 6638-6647

    • Related Report
      2010 Final Research Report
  • [Journal Article] Improved protein sequence coverage by on resin deglycosylation and cysteine modification for biomarker discovery.2009

    • Author(s)
      Kamada H., Fugmann T., Neri D., Roesli C.
    • Journal Title

      Proteomics 9(3)

      Pages: 783-787

    • Related Report
      2010 Final Research Report
  • [Journal Article] Improved protein sequence coverage by on resin deglycosylation and cysteine modification for biomarker discovery.2009

    • Author(s)
      Kamada H., Fugmann T., et. al
    • Journal Title

      Proteomics 9

      Pages: 783-787

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The treatment of established murine collagen-induced arthritis with a TNFR1-selective antagonistic mutant TNF.2009

    • Author(s)
      Shibata H., Kamada H., et. al
    • Journal Title

      Biomaterials 30

      Pages: 6638-6647

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function relationship of tumor necrosis factor (TNF) and its receptor interaction based on 3D structural analysis of a fully active TNFR 1-selective TNF mutant.2009

    • Author(s)
      Mukai Y., Kamada H., et. al
    • Journal Title

      J.Mol.Biol. 385

      Pages: 1221-1229

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The specific effect of 2-Methoxyestradiol on lymphatic vascular endothelial cells.2009

    • Author(s)
      Imai S., Kamada H., et. al
    • Journal Title

      Pharmazie 64

      Pages: 214-216

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid isolation of intrabody candidates by using an optimized non-immune phage antibody library.2009

    • Author(s)
      Nagano K., Kamada H., et. al
    • Journal Title

      Pharmazie 64

      Pages: 238-241

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of TNF-TNFR2 complex.2009

    • Author(s)
      Mukai Y., Kamada H., et. al
    • Journal Title

      Acta.Crystallogr.Sect.F. 65(Pt.3)

      Pages: 295-298

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The augmentation of intracellular delivery of peptide therapeutics by artificial protein transduction domains.2009

    • Author(s)
      Yoshikawa T., Kamada H., et.al
    • Journal Title

      Biomaterials 30

      Pages: 3318-3323

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF : useful information for designing artificial proteo-antagonists.2009

    • Author(s)
      Mukai Y., Kamada H., et. al
    • Journal Title

      J.Biochem 146

      Pages: 167-172

    • NAID

      10026302534

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TNF superfamily member, TLIA, is a potential mucosal vaccine adjuvant.2009

    • Author(s)
      Kayamuro H., Kamada H., et.al
    • Journal Title

      Biochem.Biophys.Res.Commun. 384

      Pages: 296-300

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic trioxide has the inhibitory effect on transmission of human T-cell leukemia virus type 12009

    • Author(s)
      Nabeshi H., Kamada H., et. al
    • Journal Title

      Biol.Pharm.Bull. 32

      Pages: 1286-1288

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The use of a mutant TNF-alpha as a vaccine adjuvant for the induction of mucosal immune responses.2009

    • Author(s)
      Kayamuro H., Kamada H., et. al
    • Journal Title

      Biomaterials 29

      Pages: 5869-5873

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel protein engineering strategy for creating highly receptor-selective mutant TNFs.2009

    • Author(s)
      Nomura T., Kamada H., et. al
    • Journal Title

      Biochem.Biophys.Res.Commun. 388

      Pages: 667-671

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 抗体工学を駆使した創薬ターゲットの探索技術2011

    • Author(s)
      鎌田春彦
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 抗体工学を駆使した創薬ターゲットの探索技術2011

    • Author(s)
      鎌田春彦
    • Organizer
      日本薬学会 第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] イオンモビリティー質量分析法を用いた抗体医薬品の構造とその生物活性の変化に関する基礎検討2010

    • Author(s)
      鎌田春彦, 廣瀬賢治, 押方基二, 佐藤太, 長野一也, 阿部康弘, 堤康央, 角田慎一
    • Organizer
      第10回日本蛋白質科学会
    • Place of Presentation
      北海道
    • Related Report
      2010 Final Research Report
  • [Presentation] イオンモビリティー質量分析法を用いた抗体医薬品の構造とその生物活性の変化に関する基礎検討2010

    • Author(s)
      鎌田春彦, など
    • Organizer
      第10回 日本蛋白質科学会
    • Place of Presentation
      北海道
    • Related Report
      2010 Annual Research Report
  • [Presentation] イオンモビリティー質量分析法を用いた蛋白質医薬品の品質管理に向けた基礎検討.2009

    • Author(s)
      鎌田春彦, ら
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] イオンモビリティー質量分析法による蛋白質医薬品の構造と活性の連関解析.2009

    • Author(s)
      鎌田春彦, ら
    • Organizer
      第57回日本質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] イオンモビリティー質量分析法による蛋白質医薬品の構造と活性の連関解析2009

    • Author(s)
      鎌田春彦, 廣瀬賢治, 阿部康弘, 角田慎一, 堤康央
    • Organizer
      第57回日本質量分析総合討論会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] イオンモビリティー質量分析法を用いた蛋白質医薬品の品質管理に向けた基礎検討2009

    • Author(s)
      鎌田春彦, 廣瀬賢治, 阿部康弘, 角田慎一, 堤康央
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.nibio.go.jp/bio-r/index.htm

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.nibio.go.jp/LPP_HP/index.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nibio.go.jp/LPP_HP/index.htm

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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