Analysis for cell polarity regulation by using drug inducible gene-knock-out system.

• Project/Area Number: 21790177
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kanazawa University
• Principal Investigator: NAKAYA Masa-aki Kanazawa University, 医学系, 助教 (70422095)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 細胞極性 / Cre-loxP / 発生生物学 / 形態形成学 / 分子細胞生物学

Research Abstract

In this study, we constructed novel experimental system, which enable us to disrupt gene function under the control of Tamoxifen by using Cre-loxP technology. We focused on two polarity related genes, aPKC(atypical Protein kinase C) and Daam1(Deshevelled associated activator of morphogenesis), and successfully detected genetic interaction and cellular phenotype. Further, we confirmed this results by using Xenopus injection experiment and found the possibility that aPKC acts as down stream of Daam1.

Research Products

• [Journal Article] Bone marrow-derived stromal cells can express neuronal markersby DHA/GPR40signaling. (2010)
  • Author(s): Kaplamadzhiev DB, Hisha H, Adachi Y, Ikehara S, Tonchev AB, Boneva NB, Pyko IV, Kikuchi M, Nakaya MA, Wakayama T, Iseki S, Yamashima T.
  • Journal Title: BioScience Trends Volume: 4 Pages: 119-129
  • Peer Reviewed
• [Journal Article] Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota Y., Meunier A., Huang S., Shimozawa T., Yamada O., Kida YS., Inoue M., Ito T., Kato H., Sakaguchi M., Sunabori T., Nakaya MA., Nonaka S., Ogura T., Higuchi H., Okano H., Spassky N., Sawamoto K.
  • Journal Title: Development Volume: 137 Pages: 3037-3046
  • Peer Reviewed
• [Journal Article] Expression of cAMP response element-binding protein in the duct system of the mouse submandibular gland. (2009)
  • Author(s): Keattikunpairoj S, Wakayama T, Yamamoto M, Nakaya MA, Nakata H, Hipkaeo W, Sakulsak N, lseki S.
  • Journal Title: Histochem Cell Biol. 132 Pages: 647-657
  • Peer Reviewed
• [Presentation] The role of aPKC and Daam in cell polarity regulation. (2011)
  • Author(s): 中谷雅明、井関尚一
  • Organizer: 第116回日本解剖学会総会・第88回日本生理学会大会全国学術集会合同大会
  • Place of Presentation: 神奈川県(学会中止のため The journal of Physiological Science vol.61 supplement 1, 2011, S261, P3-055 誌上発表)
  • Year and Date: 2011-03-30
• [Presentation] The role of aPKC and Daam in cell polarity regulation. (2011)
  • Author(s): 中谷雅明、井関尚一
  • Organizer: 第116回日本解剖学会総会・第88回日本生理学会大会全国学術集会合同大会
  • Place of Presentation: 神奈川県横浜市((予定)学会中止のためThe journal of Physiological Science誌上発表)
  • Year and Date: 2011-03-30
• [Presentation] 薬剤誘導性遺伝子ノックアウト法を用いた細胞極性制御機構の解析 (2009)
  • Author(s): 中谷雅明
  • Organizer: 日本解剖学会第69回中支部学術集会
  • Place of Presentation: 静岡県・浜松医科大学
  • Year and Date: 2009-10-10
• [Presentation] 薬剤誘導性遺伝子ノックアウト法を用いた細胞極性制御機構の解析 (2009)
  • Author(s): 中谷雅明
  • Organizer: 日本解剖学会第69回中支部学術集会
  • Place of Presentation: 静岡県浜松市浜松医科大学
  • Year and Date: 2009-10-10
• [Remarks] ホームページ等
  • URL: http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=858
• [Remarks]
  • URL: http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=858
• [Remarks]
  • URL: http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=858