Role of arginine-methyltransferase for quality control of protein maturation
Project/Area Number |
21790181
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Osaka University |
Principal Investigator |
MATSUZAKI Shinsuke Osaka University, 連合小児発達学研究科, 准教授 (60403193)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 小胞体ストレス / アルギニンメチル化酵素 / タンパク質品質管理 / ジメチルアルギニン / PRMT1 / 蛋白質品質管理 |
Research Abstract |
We discovered upregulation of Protein Arginine Methyltransferases (PRMT) under endoplasmic reticulum (ER) stress condition. Protein level of PRMT1 is increased by ER stressor tunicamycin, time-dependently. PRMT1 is upregulated by XBP1 overexpression. In contrast, cells knocked-down (KD) of PRMT1 showed increase of ER-chaperone GRP78 under untreated condition (non-stress condition). Moreover, these cells cause abnormal localization Golgi complex, resulting increase of the Golgi-related proteins such as GM130, TGN38, Fut8. Finally, to transfect PRMT1-YFP into PRMT1-KD cells, the abnormal accumulation of Golgi complex was recovered.These results suggest that PRMT1 regulates the structure and the function of Golgi complex, associated to ER function.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] DISC1 regulates cell-cell adhesion, cell-matrix adhesion and neurite outgrowth.2010
Author(s)
Hattori T, Shimizu S, Koyama Y, Yamada K, Kuwahara R, Kumamoto N, Matsuzaki S, Ito A, Katayama T, Tohyama M.
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Journal Title
Molecular Psychiatry 15(8)
Related Report
Peer Reviewed
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[Journal Article] Dysbindin regulates the transcriptional level of myristoylated alanine-rich protein kinase C substrate via the interaction with NF-YB in mice brain.2010
Author(s)
Okuda H, Kuwahara R, Matsuzaki S, Miyata S, Kumamoto N, Hattori T, Shimizu S, Yamada K, Kawamoto K, Hashimoto R, Takeda M, Katayama T, Tohyama M.
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Journal Title
PLoS ONE.(corresponding author and equally contributed author) 5(1)
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Peer Reviewed
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[Journal Article] Increased stathminl expression in the dentate gyrus of mice causes abnormal axonal arborizations.2010
Author(s)
Yamada K, Matsuzaki S, Hattori T, Kuwahara R, Taniguchi M, Hashimoto H, Shintani N, Baba A, Kumamoto N, Yamada K, Yoshikawa T, Katayama T, Tohyama M.
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[Journal Article] Increased stathminl expression in the dentate gyrus of mice causes a bnormal axonal arborizations.2010
Author(s)
Yamada K, Matsuzaki S, Hattori T, Kuwahara R, Taniguchi M, Hashimoto H, Shintani N, Baba A, Kumamoto N, Yamada K, Yoshikawa T, Katayama T, Tohyama M
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Related Report
Peer Reviewed
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[Journal Article] Dysbindin regulates the transcriptional level of myristoylated alanine-rich protein kinase C substrate via the interaction with NF-YB in mice brain.2010
Author(s)
Okuda H, Kuwahara R, Matsuzaki S Miyata S, Kumamoto N, Hattori T, Shimizu S, Yamada K, Kawamoto K, Hashimoto R, Takeda M, Katayama T, Tohyama M.
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Peer Reviewed
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[Journal Article] Association studies and gene expression analyses of the DISCl-interacting molecules, pericentrin 2 (PCNT2) and DISCl-binding zinc finger protein (DBZ), with schizophrenia and with bipolar disorder.2009
Author(s)
Anitha A, Nakamura K, Yamada K, Iwayama Y, Toyota T, Takei N, Iwata Y, Suzuki K, Sekine Y, Matsuzaki H, Kawai M, Thanseem I, Miyoshi K, Katayama T, Matsuzaki S, Baba K, Honda A, Hattori T, Shimizu S, Kumamoto N, Kikuchi M, Tohyama M, Yoshikawa T, Mori N.
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Journal Title
Am J Med Genet B Neuropsychiatr Genet. 150B(7)
Pages: 967-76
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Peer Reviewed
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[Journal Article] Association studies and gene expression analyses of the DISC1-interac ting molecules, pericentrin 2 (PCNT2) and DISC1-binding zinc finger protein (DBZ), with schizophrenia and with bipolar disorder.2009
Author(s)
Anitha A, Nakamura K, Yamada K, Iwayama Y, Toyota T, Takei N, Iwata Y, Suzuki K, Sekine Y, Matsuzaki H, Kawai M, Thanseem I, Miyoshi K, Katayama T, Matsuzaki S, Baba K, Honda A, Hattori T, Shimizu S, Kumamoto N, Kikuchi M, Tohyama M, Yoshikawa T, Mori N.
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Journal Title
Am J Med Genet B Neuropsychiatr Genet. 150B(7)
Pages: 967-976
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Peer Reviewed
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[Presentation] ERストレス下での翻訳後修飾2009
Author(s)
桑原隆亮, 松崎伸介, 服部剛志, 森泰丈, 河本恵介, 清水尚子, 山田浩平, 片山泰一, 遠山正彌
Organizer
第32回 神経科学大会
Place of Presentation
名古屋
Year and Date
2009-09-17
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