| Project/Area Number |
21790199
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TOYODA Futoshi Shiga University of Medical Science, 医学部, 助教 (90324574)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 生体膜 / チャネル / トランスポーター / 能動輸送 / 生理学 / 生体分子 / 細胞・組織 / 循環器 / イオンチャネル / ペースメーカー |
|---|
| Research Abstract |
In the present study, we examined the possibility of the functional assembly of Cav1.2 and Cav1.3 subunits to yield the sustained inward Na^+ current (I_<st>), a pacemaker current in sinoatrial node. Heterologous coexpression of Cav1.2 and Cav1.3 in HEK cells resulted in an ensemble of Ca^<2+> currents attributed to each Cav channel. On the other hand, the chimeric channel consisting of repeat I and II from Cav1.2 (or Cav1.3) and repeat III and IV from Cav1.3 (or Cav1.2) elicited a rapidly inactivating Ca^<2+> current but failed to evoke a Na^+ current similar to I_<st>. Taken together, it is not likely that the heteromeric assembly of Cav1.2 and Cav1.3 subunits underlies I_<st> in sinoatrial node.
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