| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
The purpose of the present study was to clarify the mechanisms and significance of Cl^-dynamics via intracellular organelle membranes in beating cardiomyocytes. To achieve these goals, cell physiological experiments in combination with computer simulation were performed and the following results were obtained. 1. Technique to measure the intracellular Cl^- dynamics was established. 2. Expression of CLIC2, an intracellular chloride channel, was analyzed in cell line of beating cardiomyocytes. 3. A comprehensive cardiomyocyte model was updated by incorporating pH homeostasis and detailed mitochondria to analyze dynamics of ions and energy substrates across the intracellular organelle membranes.
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