The functional analysis of autism related and BDNF secretion regulated protein CAPS2

• Project/Area Number: 21790219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SHINODA Yo The Institute of Physical and Chemical Research, 分子神経形成研究チーム, 研究員 (80403096)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生理学 / 精神科学 / 脳・神経 / BDNFCA / PS2 / 分泌 / CAPS2 / BDNF / GABA / Interneuron / interneuron / anxiety / secretion

Research Abstract

Brain derived neurotrophic factor (BDNF) plays critical role in neurogenesis, neuronal survival and development and in synaptic development and plasticity. However, the mechanism of the BDNF secretion is largely unknown. Recently, Ca^<2+> dependent activator protein for secretion 2 (CAPS2) has been discovered as a protein which regulates BDNF secretion. In the present study, we demonstrated that CAPS2 not only accelerates BDNF secretion kinetics but also increases BDNF quantal release by using time-lapse BDNF secretion imaging technique. In addition, CAPS2 regulated BDNF secretion plays a critical role in development of GABAergic interneuron network. The GABAergic interneuron impairments affect synaptic plasticity and anxiety-like behavior.

Research Products

• [Journal Article] Expression of the IP (3) R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum. (2010)
  • Author(s): Furutama D, Morita N, Takano R, Sekine Y, Sadakata T, Shinoda Y, Hayashi K, Mishima Y, Mikoshiba K, Hawkes R, Furuichi T.
  • Journal Title: J.Neurosci,Res. 88 Pages: 2810-2825
  • Peer Reviewed
• [Journal Article] Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons. (2010)
  • Author(s): Shinoda Y, Tanaka T, Tominaga-Yoshino K, Ogura A.
  • Journal Title: PLoS ONE 5(4)
  • Peer Reviewed
• [Journal Article] Expression of the IP(3)R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum. (2010)
  • Author(s): Furutama D
  • Journal Title: J.Neurosci, Res. Volume: 88 Pages: 2810-2825
  • Peer Reviewed
• [Journal Article] Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons. (2010)
  • Author(s): Shinoda Y
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Presentation] Imaging analysis of the secretory vesicle-associated protein CAPS2 regulated BDNF secretion. (2010)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: Neuro2010
  • Place of Presentation: Kobe, Japan.
  • Year and Date: 2010-09-02
• [Presentation] Imaging analysis of the secretory vesicle-associated protein CAPS2 regulated BDNF secretion. (2010)
  • Author(s): Yo Shinoda
  • Organizer: Neuro2010
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-09-02
• [Presentation] Vesicle associated protein CAPS2 KO mice show impaired synapse function of hippocampus and exhibit depression-like behavior. (2010)
  • Author(s): Yo Shinoda
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-08-01
• [Presentation] The imaging analysis of secretory vesicle associated protein CAPS2 regulated BDNF secretion in mouse hippocampus.-The effects of CAPS2 regulated BDNF secretion for GABAergic neuronal network development-. (2010)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: The comprehensive brain summer workshop.
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 2010-07-28
• [Presentation] The imaging analysis of secretory vesicle associated protein CAPS2 regulated BDNF secretion in mouse hippocampus. -The effects of CAPS2 regulated BDNF secretion for GABAergic neuronal network development- (2010)
  • Author(s): Yo Shinoda
  • Organizer: The comprehensive brain summer workshop.
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-07-02
• [Presentation] Enhancement of BDNF secretion kinetics by the secretory vesicle-associated protein CAPS2 : reduced BDNF levels, impaired development of hippocampal GABAergic neurons and increased anxiety-like behavior in CAPS2 KO mice (2010)
  • Author(s): Yo Shinoda
  • Organizer: The 87^<th> annual meeting of the Physiological Society of Japan.
  • Place of Presentation: Morioka, Japan
  • Year and Date: 2010-05-19
• [Presentation] Enhancement of BDNF secretion kinetics by the secretory vesicle-associated protein CAPS2 : reduced BDNF levels, impaired development of hippocampal GABAergic neurons and increased anxiety-like behavior in CAPS2 KO mice. (2010)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: The 87^<th> annual meeting of the Physiological Society of Japan.
  • Place of Presentation: Morioka, Japan.
  • Year and Date: 2010-05-17
• [Presentation] Secretory vesicle-related gene CAPS2 knock-out mice exhibit the reduced number of hippocampal GABAergic interneuron and the impairments in synaptic plasticity and behavior. (2009)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: SfN meeting
  • Place of Presentation: Chicago IL, USA.
  • Year and Date: 2009-10-20
• [Presentation] Secretory vesicle-related gene CAPS2 knock-out mice exhibit the reduced number of hippocampal GABAergic interneuron and the impairments in synaptic plasticity and behavior (2009)
  • Author(s): 篠田陽
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2009-10-20
• [Presentation] Secretory vesicle-related gene CAPS2 knock-out mice exhibit the reduced number of hippocampal GABAergic interneuron and the impairments in synaptic plasticity and behavior. (2009)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: Neuro2009
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2009-09-17
• [Presentation] Secretory vesicle-related gene CAPS2 knock-out mice exhibit the reduced number of hippocampal GABAergic interneuron and the impairments in synaptic plasticity and behavior (2009)
  • Author(s): 篠田陽
  • Organizer: 日本神経科学学会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-09-17
• [Presentation] Vesicle associated protein CAPS2 KO mice show impaired synapse function of hippocampus and exhibit depression-like behavior. (2009)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: IUPS
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2009-08-01
• [Presentation] Vesicle associated protein CAPS2 KO mice show impaired synapse function of hippocampus and exhibit depression-like behavior (2009)
  • Author(s): 篠田陽
  • Organizer: IUPS
  • Place of Presentation: 京都府京都市
  • Year and Date: 2009-08-01
• [Presentation] Secretion-associated protein CAPS2 KO mice show impairments in BDNF expression and synapse function in hippocampus and exhibit anxiety-like and/or depression-like behaviors. (2009)
  • Author(s): Yo Shinoda, Tetsushi Sadakata, Asako Furuya, Ritsuko Katoh-Semba, Teiichi Furuichi.
  • Organizer: Gordon Research Conference
  • Place of Presentation: New port RI, USA.
• [Presentation] Secretion-associated protein CAPS2 KO mice show impairments in BDNF expression and synapse function in hippocampus and exhibit anxiety-like and/or depression-like behaviors (2009)
  • Author(s): 篠田陽
  • Organizer: Gordon Conference
  • Place of Presentation: Newport, RI, USA
• [Book] 有芯小胞の分泌制御因子CAPS2と自閉症感受性実験医学(増刊)Vol.28 No.5 (2010)
  • Author(s): 定方哲史、篠田陽、林周宏、古市貞一
• [Book] 自閉症の感受性候補遺伝子と動物モデル脳と精神の医学20巻4号 (2009)
  • Author(s): 篠田陽、定方哲史、林周宏、古市貞一
• [Remarks] ホームページ等