Development of FRET probe for detection of G protein activity.

• Project/Area Number: 21790239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: MASUDA Kazuyuki The University of Tokyo, 先端科学技術研究センター, 助教 (20345151)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 受容体 / チャネル / 輸送系 / シグナル伝達 / RGS / FRET

Research Abstract

The purpose of this study is to develop FRET probe for detecting the activation of heterotrimeric G protein in living cells. The developed FRET probe couples to GPCR like as wild type G protein, shows ligand-induced FRET signal. The leukemia cell line expressed the FRET probe shows membrane protrusion subsequently to G protein activation during chemotaxis.

Research Products

• [Journal Article] Soft X-ray Laser Microscopy of Lipid Rafts towards GPCR-Based Drug Discovery Using Time-Resolved FRET Spectroscopy (2011)
  • Author(s): Motoyoshi Baba, Tohru Kozasa, Takao Hamakubo, Hiroto Kuroda, Kazuyuki Masuda, Shin Yoneya, Tatsuhiko Kodama
  • Journal Title: Pharmaceuticals 4(3) Pages: 524-550
• [Journal Article] Soft X-ray Laser Microscopy of Lipid Rafts towards GPCR-Based Drug Discovery Using Time-Resolved FRET Spectroscopy (2011)
  • Author(s): Motoyoshi Baba, Tohru Kozasa, Takao Hamakubo, Hiroto Kuroda, Kazuyuki Masuda, Shin Yoneya, Tatsuhiko Kodama
  • Journal Title: Pharmaceuticals Volume: 4 Pages: 524-550
  • Peer Reviewed
• [Presentation] 三量体Gタンパク質活性化とRGS相互作用のFRETによる生細胞イメージング (2010)
  • Author(s): 増田一之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 三量体Gタンパク質活性化とRGS相互作用のFRETによる生細胞イメージング (2010)
  • Author(s): 増田一之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Remarks] ホームページ等
  • URL: http://www.lsbm.org
• [Remarks]
  • URL: http://www.lsbm.org/