High-throughput screening to identify novel factors for reprogramming

• Project/Area Number: 21790271
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: MAEKAWA Momoko Kyoto University, ウイルス研究所, 助教 (50528742)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / iPS細胞 / HTS / 遺伝子 / 化合物 / 天然物

Research Abstract

Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of transcription factors containing protooncogenes. In this study, we screened natural product, chemical, and cDNA libraries for their ability to replace the protooncogenes. We identified novel factors for reprogramming and clarified reprogramming mechanism in part.

Research Products

• [Presentation] Identification of novel reprogramming factors for induced pluripotent stem cell generation. (2010)
  • Author(s): Momoko Maekawa
  • Organizer: ISSCR 8^<th> Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-18
• [Presentation] Identification of novel reprogramming factors for induced pluripotent stem cell generation. (2010)
  • Author(s): Momoko Mekawa
  • Organizer: ISSCR 8^<th> Annual Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2010-06-18
• [Presentation] ハイスループットスクリーニングによる新規多能性誘導因子の探索 (2010)
  • Author(s): 前川桃子
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2010-03-18
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Patent(Industrial Property Rights)] Method of Efficiently Establishing Induced Pluripotent Stem Cells (2010)
  • Inventor(s): 山中伸弥, 五島直樹, 前川桃子, 河村義史, 望月宏美
  • Industrial Property Rights Holder: 国立大学法人京都大学/産業技術総合研究所/バイオ産業情報化コンソーシアム
  • Filing Date: 2010-09-03
  • Overseas
• [Patent(Industrial Property Rights)] Novel Nuclear Reprogramming Substance (2010)
  • Inventor(s): 山中伸弥, 五島直樹, 前川桃子, 河村義史, 望月宏美
  • Industrial Property Rights Holder: 国立大学法人京都大学/産業技術総合研究所/バイオ産業情報化コンソーシアム
  • Filing Date: 2010-02-19
  • Overseas
• [Patent(Industrial Property Rights)] Method of Efficiently Establishing Induced Pluripotent Stem Cells (2010)
  • Inventor(s): 山中伸弥/五島直樹/前川桃子/河村義史/望月宏美
  • Industrial Property Rights Holder: 国立大学法人京都大学/産業技術総合研究所/バイオ産業情報化コンソーシアム
  • Filing Date: 2010-09-03
  • Overseas
• [Patent(Industrial Property Rights)] Novel nuclear reprogramming substance (2010)
  • Inventor(s): 山中伸弥/五島直樹/前川桃子/河村義史/望月宏美
  • Industrial Property Rights Holder: 国立大学法人京都大学/産業技術総合研究所/バイオ産業情報化コンソーシアム
  • Filing Date: 2010-02-19
  • Overseas
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立方法 (2010)
  • Inventor(s): 山中伸弥/五島直樹/前川桃子/河村義史/望月宏美
  • Industrial Property Rights Holder: 国立大学法人京都大学/産業技術総合研究所/バイオ産業情報化コンソーシアム
  • Filing Date: 2010-02-16
  • Overseas