| Project/Area Number |
21790284
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIMA Yuichi Kyushu University, 医学研究院, 助教 (80425420)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子 / ゲノム / 発現制御 / 発生 / 分化 / マイクロアレイ / ライディッヒ細胞 / テストステロン / エンハンサー / 核内受容体 / 内分泌 / トランスジェニックマウス / Ad4BP / SF-1 / 転写 |
|---|
| Research Abstract |
We have identified fetal Leydig cell-specific enhancer of the Ad4BP/SF-1 gene, and established the mouse lines in which EGFP or Cre is expressed specifically in the fetal Leydig cells. In order to identify mRNA expression profile of the fetal Leydig cells, we performed cell sorting and microarray analyses. In addition, we intercrossed Cre-expressing mice with the SF-1 flox mice, and generated the fetal Leydig cell-specific Ad4BP/SF-1 KO mice. From the analyses of these mice, we have identified that Ad4BP/SF-1 is essential for the function and differentiation of the fetal Leydig cells, and also for the masculinization of the fetuses.
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