Roles of phospholipase C deltal in hair shaft formation

• Project/Area Number: 21790292
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: NAKAMURA Yoshikazu Tokyo University of Pharmacy and Life Science, 生命科学部, 講師 (60366416)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 毛形成 / ホスホリパーゼC / FOXN1 / Notch

Research Abstract

A transcription factor, FOXN1 is essential for hair formation. Phospholipase C (PLC)δ1, a key molecule in the phosphoinositide signaling pathway, is essential for normal hair formation. In this study, we found that Foxn1-mediated expression of PLCδ1 is important in normal hair formation and Notchl expression.

Research Products

• [Journal Article] Phospholipase C{delta}3 Regulates RhoA/Rho Kinase Signaling and Neurite Outgrowth. (2011)
  • Author(s): Kouchi Z., Igarashi T., Shibayama N., Inanobe S., Sakurai K., Yamaguchi H., Fukuda T., Yanagi S., Nakamura Y., Fukami K.
  • Journal Title: J.Biol.Chem. 286 Pages: 8459-8471
  • Peer Reviewed
• [Journal Article] Phospholipase C is a key enzyme regulating intracellular calcium and modulating phosphoinositide balance. (2010)
  • Author(s): Fukami K., Inanobe S., Kanemaru K., Nakamura Y.
  • Journal Title: Prog Lipid Res.(Review) 49 Pages: 429-37
• [Journal Article] Phosphatidylinositol 4,5-bisphosphate and PIP5-kinase Iα are required for invadopodia formation in human breast cancer cells. (2010)
  • Author(s): Yamaguchi H., Yoshida S., Muroi E., Kawamura M., Kouchi Z., Nakamura Y., Sakai R., Fukami K.
  • Journal Title: Cancer Sci. 101 Pages: 1632-8
  • NAID: 10026594611
  • Peer Reviewed
• [Journal Article] Phospholipase C-eta2 is highly expressed in the habenula and retina. (2010)
  • Author(s): Kanemaru K., Nakahara M., Nakamura Y., Hashiguchi Y., Kouchi Z., Yamaguchi H., Oshima N., Kiyonari H., Fukami K.
  • Journal Title: Gene Expr.Patterns. 10 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Phosphatidylinositol 4,5-bisphosphate and PIP5-kinase Iα are required for invadopodia formation in human breast cancer cells. (2010)
  • Author(s): Yamaguchi, H., et al
  • Journal Title: Cancer Sci. Volume: 101 Pages: 1632-38
  • Peer Reviewed
• [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase signaling and neurite outgrowth. (2010)
  • Author(s): Kouchi, Z., et al
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 8459-71
  • Peer Reviewed
• [Journal Article] Phospholipase Cδ3 is a novel binding partner of Myosin VI and functions as anchoring of Myosin VI on plasma membrane. (2010)
  • Author(s): Sakurai, K., et al
  • Journal Title: Advan.Enzyme Regul. Volume: (IN PRESS)
  • Peer Reviewed
• [Journal Article] Expression of snail in epidermal keratinocytes promotes cutaneous inflammation and hyperplasia conducive to tumor formation. (2010)
  • Author(s): Fei, D., Nakamura, Y., et al.equally contributed.
  • Journal Title: Cancer Res. Volume: 70 Pages: 10080-9
  • Peer Reviewed
• [Journal Article] Phospholipase C-eta2 is highly expressed in the habenula and retina. (2010)
  • Author(s): Kanemaru K, Nakahara M, Nakamura Y, Hashiguchi Y, Kouchi Z, Yamaguchi H, Oshima N, Kiyonari H, Fukami K.
  • Journal Title: Gene Expr. Patterns. 10(2-3) Pages: 119-126
  • Peer Reviewed
• [Journal Article] Roles of phospholipase C isozymes in organogenesis and embryonic development. (2009)
  • Author(s): Nakamura Y., Fukami K.
  • Journal Title: Physiology(Review) 24 Pages: 332-41
• [Journal Article] Lipid rafts and caveolin-1 are required for invadopodia formation and extracellular matrix degradation by human breast cancer cells. (2009)
  • Author(s): Yamaguchi H., Takeo Y., Yoshida S., Kouchi Z., Nakamura Y., Fukami K.
  • Journal Title: Cancer Res. 69 Pages: 8594-602
  • Peer Reviewed
• [Journal Article] Roles of phospholipase C isozymes in organogenesis and embryonic development. (2009)
  • Author(s): Nakamura Y, Fukami K.
  • Journal Title: Physiology 24 Pages: 332-341
  • Peer Reviewed
• [Journal Article] Lipid rafts and caveolin-1 are required for invadopodia formation and extracellular matrix degradation by human breast cancer cells. (2009)
  • Author(s): Yamaguchi H, Takeo Y, Yoshida S, Kouchi Z, Nakamura Y, Fukami K.
  • Journal Title: Cancer Res. 69 Pages: 8594-8602
  • Peer Reviewed
• [Journal Article] Expression of Snail in epidermal keratinocytes promotes cutaneous inflammation and hyperplasia conducive to tumor formation.
  • Author(s): Du F.(*), Nakamura Y.(*), Tan T.L., Lee P., Lee R., Yu B., Jamora C.
  • Journal Title: Cancer Res. 70(in press) Pages: 10080-9
  • Peer Reviewed
• [Journal Article] Phospholipase Cδ3 is a novel binding partner of Myosin VI and functions as anchoring of Myosin VI on plasma membrane.
  • Author(s): Sakurai K., Hirata M., Yamaguchi H., Nakamura Y., Fukami K.
  • Journal Title: Advan.Enzyme Regul. in press
  • Peer Reviewed
• [Presentation] 表皮におけるホスホリパーゼC deltalの欠損は全身性の顆粒球増加を引き起こす (2010)
  • Author(s): 中村由和、金丸佳織、深見希代子
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 表皮におけるホスホリパーゼCdelta1の欠損は全身性の顆粒球増加を引き起こす (2010)
  • Author(s): 中村由和, 金丸佳織, 深見希代子
  • Organizer: BMB2010(ワークショップ)
  • Place of Presentation: 神戸
• [Presentation] ホスホリパーゼCδ1は正常な白血球数維持に重要である (2010)
  • Author(s): 金丸佳織, 中村由和, 児島亮太, 深見希代子
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] 肥満形成におけるホスホリパーゼCδ1の生理的役割 (2010)
  • Author(s): 鈴木睦実, 平田真之, 石井里佳, 中村由和, 深見希代子
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] ホスホリパーゼC-delta1はヌード遺伝子Foxn1下流において正常な毛の形成を制御している (2010)
  • Author(s): 高橋佐織, 中村由和, 深見希代子
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] PLCδ1は正常な白血球数の維持に重要である (2010)
  • Author(s): 金丸佳織、藤森亮、児島亮太、中村由和、深見希代子
  • Organizer: 第52回脂質生化学会
  • Place of Presentation: 群馬
• [Presentation] ホスホリパーゼC-delta1による毛形成制御機構 (2010)
  • Author(s): 高橋佐織、中村由和、一戸学、深見希代子
  • Organizer: 第52回脂質生化学会
  • Place of Presentation: 群馬
• [Presentation] 肥満におけるホスホリパーゼCδ1の生理機能解析 (2010)
  • Author(s): 鈴木睦実、平田真之、石井里佳、中村由和、深見希代子
  • Organizer: 第52回脂質生化学会
  • Place of Presentation: 群馬
• [Presentation] Phospholipase C-deltal Regulates Hair Keratin Expression And Hair Shaft Formation Downstream of Foxnl. (2009)
  • Author(s): Y.Nakamura ; M.Ichinohe ; S.Takahashi ; S.Inanobe ; K.Kanemaru ; K.Fukami
  • Organizer: The American Society for Cell Biology 49th Annual meeting
  • Place of Presentation: Convention center, San Diego, USA
  • Year and Date: 2009-12-07
• [Presentation] ホスホリパーゼC delta1はFoxn1下流に位置し毛の形成を制御している (2009)
  • Author(s): 中村由和, 一戸学, 平田真之, 高橋佐織, 深見希代子
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] PHYSIOLOGICAL FUNCTIONS OF BIO ACTIVE LIPID PRODUCER, PHOSPHOLIPASE C-DELTA1 IN SKIN (2009)
  • Author(s): Nakamura Y., Fukami K.
  • Organizer: 4^<th> INTERNATIONAL CONFERENCE ON PHOS PHOLIFASE A_2 AND LIPID MEDIATORS
  • Place of Presentation: 学術総合センター
  • Year and Date: 2009-05-28
• [Presentation] Phospholipase C-delta1 Regulates Hair Keratin Expression And Hair Shaft Formation Downstream of Foxn1 (2009)
  • Author(s): Nakamura, Y., Ichinohe, M., Takahashi, S., Inanobe, S., Kanemaru, K., Fukami, K.
  • Organizer: The American Society for Cell Biology 49th Annual meeting
  • Place of Presentation: San Diego
• [Presentation] Physiological functions of phospholipase C δ1 in adipose tissue development and obesity (part1) (2009)
  • Author(s): Ishii, R., Hirata, M., Suzuki, M., Matsuoka, S., Nakamura, Y., Kouchi, Z., Yamaguchi, H., Sasaki, T., Kitamurea, T., Fukami, K.
  • Organizer: The 6th Japan-Korea Conference on celluer signaling for young scientists
  • Place of Presentation: Nagasaki
• [Presentation] Phospholipase C δ3 is involved in neurite outgrowth of cerebral cortical neurons and Neuro2A cells (2009)
  • Author(s): Shibayama, N., Kouchi, Z., Fukuda, T., Igrashi, T., Nakamura, Y., Yamaguchi, H., Yanagi, S., Fukami, K.
  • Organizer: The 6th Japan-Korea Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: Nagasaki
• [Presentation] Lack of phospholipase C delta1 accelerates skin wound healing (2009)
  • Author(s): Inanobe, S., Nakamura, Y., Ichinohe, M., Hirata, M., Kouchi, Z., Yamaguchi, H., Fukami, K.
  • Organizer: The 6th Japan-Korea Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: Nagasaki
• [Presentation] ホスフォリパーゼCδ1は様々な生理機能に関与する (2009)
  • Author(s): 深見希代子、平田真之、石井里佳、一戸学、中村由和
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸(シンポジウム)
• [Presentation] ホスホリパーゼCdelta1はFoxn1下流に位置し毛の形成を制御している (2009)
  • Author(s): 中村由和、一戸学、平田真之、高橋佐織、深見希代子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 大腸癌細胞におけるvitaminDレセプター依存的なE-cadherinの発現誘導にPIP kinase IIβが関与する (2009)
  • Author(s): 河内全、小堀真平、宮崎聡一郎、中村由和、山口英樹、深見希代子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 白血球の機能、分化におけるホスホリパーゼδ1の機能解析 (2009)
  • Author(s): 金丸佳織、中村由和、河内全、山口英樹、深見希代子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] PLCδ1による毛包形態および毛の構成ケラチン発現の制御 (2009)
  • Author(s): 高橋佐織、一戸学、中村由和、平田真之、河内全、山口英樹、深見希代子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 脂肪細胞形成におけるホスホリパーゼCδ1の生理機能解析 (2009)
  • Author(s): 石井里佳、平田真之、鈴木睦美、松岡翔一、中村由和、河内全、山口英樹、深見希代子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 創傷治癒におけるホスポリパーゼCδ1の役割 (2009)
  • Author(s): 稲野邊俊一、中村由和、一戸学、平田真之、河内全、山口英樹、深見希代子
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: 名古屋
• [Presentation] Neuro2a細胞の神経突起伸長におけるPLCδ3の機能解析 (2009)
  • Author(s): 河内全、五十嵐隆公、柴山奈美、中原真道、中村由和、山口英樹、深見希代子
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: 名古屋
• [Presentation] 浸潤突起形成における脂質ラフトとカベオリンの役割 (2009)
  • Author(s): 山口英樹、竹尾由希子、吉田周平、河内全、中村由和、深見希代子
  • Organizer: 第61回日本細胞生物学会(ワークショップ)
  • Place of Presentation: 名古屋
• [Presentation] Phospholipase Cδ3はNeuro2a細胞における神経突起伸長に関与する (2009)
  • Author(s): 柴山奈美、河内全、五十嵐隆公、中村由和、山口英樹、深見希代子
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 肥満におけるホスホリパーゼCdeltalの生理機能解析 (2009)
  • Author(s): 平田真之、石井里佳、一戸学、中村由和、河内全、山口英樹、深見希代子
  • Place of Presentation: 名古屋
• [Presentation] physiological functions of a bioactive lipid producer,phospholipase c-delta 1 in skin (2009)
  • Author(s): Nakamura, Y.
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: Tokyo
• [Book] 遺伝子改変マウスの表現型より示されるホスホリパーゼCの生理的役割(実験医学増刊分子から個体へと深化する脂質生物学)(28(20))
  • Author(s): 中村由和、深見希代子
  • Publisher: 羊土社