Generation of conditional knockout of small Maf genes in mice

• Project/Area Number: 21790303
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: KATSUOKA Fumiki Tohoku University, 大学院・医学系研究科, 准教授 (30447255)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 転写因子 / 疾患モデルマウス / 小Maf群因子 / 生体防御

Research Abstract

CNC-small Maf heterodimers are important for the regulation of various cytoprotective genes. This study revealed that small Maf proteins are indispensable for embryogenesis after E9.5, especially for liver development. This study also showed that the neurodegeneration observed in small Maf mutant mice is cell-autonomous effect. Conditional knockout system of small Maf proteins was established and will enable us to examine the function of small Maf proteins in adult tissues.

Research Products

• [Journal Article] Central nervous system-specific deletion of transcription factor Nrf1 dcauses progressive motor neuronal dysfunction. (2011)
  • Author(s): kira Kobayashi, Takako Tsukide, Tomohiro Miyasaka, Tomoko Morita, Tatsuya Mizoroki, Yoshiro Saito, Yasuo Ihara, Akihiko Takashima, Noriko Noguchi, Akiyoshi Fukamizu, Yosuke Hirotsu, Makiko Ohtsuji, Fumiki Katsuoka, Masayuki Yamamoto.
  • Journal Title: Genes Cells 印刷中
  • Peer Reviewed
• [Journal Article] Nrf2 degron-fused reporter system ; a new tool for specific evaluation of Nrf2 inducers. (2011)
  • Author(s): Yosuke Hirotsu, Fumiki Katsuoka, Ken Itoh, Masayuki Yamamoto.
  • Journal Title: Genes Cells 16 Pages: 406-415
  • Peer Reviewed
• [Journal Article] Molecular determinants for small Maf protein control of platelet production. (2011)
  • Author(s): Hozumi Motohashi, Rie Fujita, Mariko Takayama, Ai Inoue, Fumiki Katsuoka, Emery H.Bresnick, Masayuki Yamamoto.
  • Journal Title: Mol Cell Biol. 31 Pages: 151-162
  • Peer Reviewed
• [Journal Article] Nrf2 degron-fused reporter system ; a new tool for specific evaluation of Nrf2 inducers. (2011)
  • Author(s): Yosuke Hirotsu, Fumiki Katsuoka, Ken Itoh, Masayuki Yamamoto.
  • Journal Title: Genes Cells Volume: 16 Pages: 406-415
  • Peer Reviewed
• [Journal Article] Central nervous system-specific deletion of transcription factor Nrf1 causes progressive motor neuronal dysfunction. (2011)
  • Author(s): Akira Kobayashi, Takako Tsukide, Tomohiro Miyasaka, Tomoko Morita, Tatsuya Mizoroki, Yoshiro Saito, Yasuo Ihara, Akihiko Takashima, Noriko Noguchi, Akiyoshi Fukamizu, Yosuke Hirotsu, Makiko Ohtsuji, Fumiki Katsuoka, Masayuki Yamamoto.
  • Journal Title: Genes Cells Volume: (未定 印刷中)
  • Peer Reviewed
• [Journal Article] Molecular determinants for small Maf protein control of platelet production. (2011)
  • Author(s): Motohashi H, Fujita R, Takayama M, Inoue A, Katsuoka F, Bresnick EH, Yamamoto M.Molecular determinants for small Maf protein control of platelet production
  • Journal Title: Mol Cell Biol Volume: 31 Pages: 151-162
  • Peer Reviewed
• [Journal Article] NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation. (2010)
  • Author(s): Hozumi Motohashi, Momoko Kimura, Rie Fujita, Ai Inoue, Xiaoqing Pan, Mariko Takayama, Fumiki Katsuoka, Hiroyuki Aburatani, Emery H.Bresnick, Masayuki Yamamoto.
  • Journal Title: Blood. 115 Pages: 677-689
  • Peer Reviewed
• [Journal Article] NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation (2010)
  • Author(s): Motohashi H, Kimura M, Fujita R, Inoue A, Pan X, Takayama M, Katsuoka F, Aburatani H, Bresnick EH, Yamamoto M.
  • Journal Title: Blood 115 Pages: 677-686
  • Peer Reviewed
• [Presentation] 転写因子Nrf1とNrf2の中枢神経系における機能的差異の検証 (2010)
  • Author(s): 弘津陽介, 勝岡史城, 小林聡, 山本雅之
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 転写因子Nrf1とNrf2の中枢神経系における機能的差異の検証 (2010)
  • Author(s): 弘津陽介, 勝岡史城, 小林聡, 山本雅之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Embryonic lethality and fetal liver hypoplasia in mice lacking all three small Maf proteins. (2010)
  • Author(s): Hiromi Yamazaki, Fumiki Katsuoka, Hozumi Motohashi, James Douglas Engel, Masayuki Yamamoto.
  • Organizer: 9th JBS Bio-Frontier Symposium 2010-5th International symposium of GATA factors.
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-17
• [Presentation] Embryonic lethality and fetal liver hypoplasia in mice lacking all three small Maf proteins. (2010)
  • Author(s): Fumiki Katsuoka, Hiromi Yamazaki, Hozumi Motohashi, James Douglas Engel, Masayuki Yamamoto
  • Organizer: 9th Japanese Biochemical Society (JBS) Bio-Frontier Symposium 2010/5th International Symposium on GATA factors.
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-17
• [Presentation] Molecular basis of CNC and small Maf dimer function in neural tissues. (2010)
  • Author(s): Fumiki Katsuoka.
  • Organizer: Jornada Cien tifica Regulatory mechanisms in inflammatory processes. University of Valencia
  • Place of Presentation: スペイン、バレンシア
  • Year and Date: 2010-10-15
• [Presentation] Molecular basis of CNC and small Maf dimer function in neural tissues. (2010)
  • Author(s): Fumiki Katsuoka
  • Organizer: Jornada Cientifica-Regulatory mechanisms in inflammatory processes
  • Place of Presentation: スペイン、バレンシア
  • Year and Date: 2010-10-15
• [Presentation] Comprehensive analysis of gene expression in central nervous system of small Maf compound mutant mice. (2009)
  • Author(s): 弘津陽介、勝岡史城、山本雅之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Nrf2 is degraded by Keap1-independent proteasome pathway in the absence of small Maf proteins. (2009)
  • Author(s): 勝岡史城、中山博未、本橋ほづみ、山本雅之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Nrf2 is degraded by Keap1-independent proteasome pathway in the absence of small Maf proteins (2009)
  • Author(s): 勝岡史城, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Comprehensive analysis of gene expression in central nervous system of small Maf compound mutant mice (2009)
  • Author(s): 弘津陽介、勝岡史城、山本雅之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Pivotal roles of small Maf proteins in the regulation of stress response genes. (2009)
  • Author(s): Katsuoka F, Motohashi H, Nakayama H, Yamamoto M.
  • Organizer: The 1st International Symposium "Challenge to Medical Innovation"
  • Place of Presentation: 仙台
  • Year and Date: 2009-12-07
• [Presentation] Nrf2 is degraded by Keap1-independent degradation pathway in the absence of small Maf proteins. (2009)
  • Author(s): Katsuoka F, Motohashi H, Nakayama H, Yamamoto M.
  • Organizer: 21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: 東京
  • Year and Date: 2009-10-26
• [Presentation] マウス胎生期における小Maf群因子の機能 (2009)
  • Author(s): 勝岡史城、中山博未、本橋ほづみ、Engel JD, 山本雅之
  • Organizer: 日本生化学会東北支部第75回例会・シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-09
• [Presentation] マウス胎生期における小Maf群因子の機能 (2009)
  • Author(s): 勝岡史城, 他
  • Organizer: 日本生化学会東北支部 第75回例会
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-09
• [Presentation] マウス胎生期における小Maf群因子の機能 (2009)
  • Author(s): 中山博未、勝岡史城、本橋ほづみ、山本雅之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Nrf2誘導剤スクリーニング系の確立 (2009)
  • Author(s): 弘津陽介、勝岡史城、山本雅之
  • Organizer: 平成21年度合同班会議「遺伝子情報デコード」
  • Place of Presentation: 富山