The dynamic regulation of an Ig-like cell adhesion molecule, CADM1 in cell adhesion and invasion

• Project/Area Number: 21790309
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAKURAI Mika 東京大学, 医科学研究所, 助教 (80508359)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞接着 / CADM1 / 動的制御 / FRAP法 / 細胞間接着 / 細胞骨格 / FRAP / FLIP / 細胞浸潤 / ダイナミクス / シグナル伝達

Research Abstract

A cell adhesion molecule, CADM1, is involved in the formation and maintenance of epithelial cell adhesion by interacting with actin-binding proteins. By analyzing the dynamic regulation of CADM1 complex using fluorescence-tagged molecules, CADM1 complex was found to be exchanged continuously in confluent cells. In contrast, in adult T-cell leukemia(ATL), CADM1 played a role in trans-endothelial migration through the formation of the protrusion at the attachment sites of ATL and endothelial cells.

Research Products

• [Journal Article] Expression of a splicing variant of the CADM1 specific to small cell lung cancer (2012)
  • Author(s): Kikuchi, S., Iwai, M., Sakurai-Yageta, M., Tsuboi, Y., Ito, T., Maruyama, T., Tsuda, H., Kanai, Y., Onizuka, M., Sato, Y., et al
  • Journal Title: Cancer Sci Volume: 103 Pages: 1051-7
  • Peer Reviewed
• [Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma (2012)
  • Author(s): Nagata, M., Sakurai-Yageta, M., Yamada, D., Goto, A., Ito, A., Fukuhara, H., Kume, H., Morikawa, T., Fukayama, M., Homma, Y., et al
  • Journal Title: Int J Cancer Volume: 130 Pages: 1329-37
  • Peer Reviewed
• [Journal Article] Expression of a splicing variant of the CADM1 specific to small cell lung cancer (2012)
  • Author(s): Kikuchi S, Iwai M, Sakurai-Yageta M, Tsuboi Y, Ito T, Maruyama T, Tsuda H, Kanai Y, 0nizuka M, Sato Y, Murakami Y
  • Journal Title: Cancer Science Volume: 103 Issue: 6 Pages: 1051-1057
  • DOI: 10.1111/j.1349-7006.2012.02277.x
  • Peer Reviewed
• [Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma (2012)
  • Author(s): Nagata M, et al.
  • Journal Title: Int.J Cancer Volume: 130 Issue: 6 Pages: 1329-37
  • DOI: 10.1002/ijc.26160
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the CADM1 gene by retinoic acid during the neural differentiation of murine embryonal carcinoma P19 cells (2011)
  • Author(s): Ito, T., Williams-Nate, Y., Iwai, M., Tsuboi, Y., Hagiyama, M., Ito, A., Sakurai-Yageta, M. and Murakami, Y
  • Journal Title: Genes Cells Volume: 16 Pages: 791-802
  • Peer Reviewed
• [Journal Article] Aberrant expression of tumor suppressors CADM1 and 4. 1B in invasive lesions of primary breast cancer (2011)
  • Author(s): Takahashi, Y., Iwai, M., Kawai, T., Arakawa, A., Ito, T., Sakurai-Yageta, M., Ito, A., Goto, A., Saito, M., Kasumi, F., et al
  • Journal Title: Breast Cancer Volume: (In press)
  • Peer Reviewed
• [Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma. (2011)
  • Author(s): Nagata M, et al.
  • Journal Title: Int J Cancer Volume: (印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Tumor suppressor CADM1 is involved in epithelial cell structure (2009)
  • Author(s): Sakurai-Yageta, M., Masuda, M., Tsuboi, Y., Ito, A. and Murakami, Y
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 390 Pages: 977-82
  • Peer Reviewed
• [Journal Article] Tumor suppressor CADM1 is involved in epithelial cell structure. (2009)
  • Author(s): Sakurai-Yageta M, et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 390(3) Pages: 977-982
  • Peer Reviewed
• [Presentation] MALDI質量分析を用いた細胞間接着分子CADM1のN型糖鎖の解析 (2011)
  • Author(s): 櫻井美佳
  • Organizer: 第59回日本質量分析学会総合討論会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-14
• [Presentation] Dynamics of CADM1 protein in the membrane of stable adhesion and in the process of cell-cell contact formation (2010)
  • Author(s): Sakurai-Yageta Mika
  • Organizer: The American Society for Cell Biology 50th Annual Meeting
  • Place of Presentation: The Pennsylvania Convention Center, U. S. A.
  • Year and Date: 2010-12-13
• [Presentation] Dynamics of CADM1 protein in the membrane of stable adhesion and in the process of cell-cell contact formation (2010)
  • Author(s): Sakurai-Yageta Mika
  • Organizer: The American Society for Cell Biology 50th Annual Meeting
  • Place of Presentation: The Pennsylvania Convention Center, U.S.A.
  • Year and Date: 2010-12-13
• [Presentation] Dynamics of CADM1 protein in the membrane of stable adhesion and in the process of cell-cell contact formation (2009)
  • Author(s): 櫻井(八下田)美佳
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/hitogan
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/hitogan
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/hitogan
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/hitogan