Investigation of neuroblastoma tumorigenesis focusing on cell cycle related genes

• Project/Area Number: 21790317
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: MURAKAMI Yuko (TONAMI Yuko) 名古屋大学, 大学院・医学系研究科, COE特任助教 (70405174)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経芽腫 / 発がん / 細胞周期 / DNA損傷 / 老化 / 合成致死 / 神経芽種

Research Abstract

We identified Smc2 and Sgo1 as genes which show synthetic lethal or senescence phenotype with MYCN amplification/overexpression. MYCN amplified neuroblastoma cell lines in which these genes are knockdowned showed the accumulation of DNA damage, but MYCN single copy cells did not. Our data indicated that Smc2 regulates various DNA repair genes transcription and Sgo1 is involved in DNA damage repair cooperation with PP2A and cohesin. Additionally, Smc2 works as condensin complex in DNA repair genes transcriptional regulation. Patients bearing neuroblastomas with amplified MYCN would be benefited from decreased Smc2 and Sgo1.

Research Products

• [Journal Article] Opposing role of condensing hinge against replication protein A in mitosis and interphase through promoting DNA annealing (2011)
  • Author(s): Akai Y, Kurokawa Y, Nakazawa N, Tonami-Murakami Y, Suzuki Y, Yoshimura SH, Iwasaki H, Shiroiwa Y, Nakamura T, Shibata E and Yanagida M.
  • Journal Title: Open Biol. Volume: 1 Pages: 110023-110023
  • Peer Reviewed
• [Journal Article] The neuronal differentiation factorNeuroD1 down regulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma (2011)
  • Author(s): Huang P, Kishida S, Cao D, Murakami-Tonami Y, Mu P, Nakaguro M, Koide N, Takeuchi I, Onishi A, Kadomatsu K.
  • Journal Title: Cancer Res. Volume: 71 Pages: 2938-48
  • Peer Reviewed
• [Journal Article] Opposing role of condensing hinge against replication protein A in mitosis and interphase through promoting DNA annealing (2011)
  • Author(s): Akai Y, Kurokawa Y, Nakazawa N, Tonami-Murakami Y, Suzuki Y, Yoshimura SH, Iwasaki H, Shiroiwa Y, Nakamura T, Shibata E, Yanagida M
  • Journal Title: Open Biology Volume: 1 Pages: 110023-110023
  • Peer Reviewed
• [Journal Article] The neuronal differentiation factor NeuroD1 downregulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma (2011)
  • Author(s): Huang P, Kishida S, Cao D, Murakami-Tonami Y, Mu P Nakaguro M, Koide N, Takeuchi I, Onishi A, Kadomatsu K
  • Journal Title: Cancer Research Volume: 71 Pages: 2938-48
  • Peer Reviewed
• [Journal Article] Regulation of yeast forkhead transcription factors and FoxM1 bycyclin-dependent and Polo-like kinases (2010)
  • Author(s): Murakami H, Aiba H, Nakanishi M and Murakami-Tonami Y.
  • Journal Title: Cell cycle. Volume: 9 Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Regulation of yeast forkhead transcription factors and FoxM1 by cyclin-dependent and Polo-like kinases (2010)
  • Author(s): Murakami H, Aiba H, Nakanishi M, Murakami-Tonami Y
  • Journal Title: Cell cycle Volume: 9 Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Casein kinase II is required for the spindle assembly checkpoint byregulatingMad2p in fission yeast (2009)
  • Author(s): Shimada M, Yamamoto A, Murakami-Tonami Y, Nakanishi M, Yoshida T, Aiba H, Murakami H.
  • Journal Title: Biochem Biophys Res Commun Volume: 388 Pages: 529-32
  • Peer Reviewed
• [Journal Article] Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast. (2009)
  • Author(s): Shimada M, Yamamoto A, Murakami-Tonami Y, Nakanishi M, Yoshida T, Aiba H, Murakami H.
  • Journal Title: Biochemical and Biophysical Research Communications 388 Pages: 529-532
  • Peer Reviewed
• [Presentation] Inactivation of hSgol showed synthetic phenotype to MYCN amplified cancer cells (2011)
  • Author(s): 村上(渡並)優子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] Inactivation of hSgo1 showed synthetic phenotype to MYCN amplified cancer cells (2011)
  • Author(s): 村上(渡並)優子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] A role of human Sgol on the growth of human neuroblastoma cells (2010)
  • Author(s): 村上(渡並)優子
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] A role of human Sgo1 on the growth of human neuroblastoma cells (2010)
  • Author(s): 村上(渡並)優子
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] A role of humanSgol on the growth of human neuroblastomacells (2010)
  • Author(s): Yuko Murakami-Tonami
  • Organizer: ANR2010
  • Place of Presentation: Stockholm City Conference Centre, Stockholm, Sweden
  • Year and Date: 2010-06-22
• [Presentation] A role of human Sgo1 on the growth of human neuroblastoma cells (2010)
  • Author(s): Yuko Murakami-Tonami
  • Organizer: ANR2010
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-06-22
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/medical/1639/1642/bunshiseibutsugaku_seitaikoubunshigaku.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/medical/1639/1642/bunshiseibutsugaku_seitaikoubunshigaku.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/medical/1639/1642/bunshiseibutsugaku_seitaikoubunshigaku.html