Establishment of KRAP-transgenic mice and elucidation of KRAP function in cancer
| Project/Area Number |
21790331
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
TAKAHIRO Fujimoto Fukuoka University, 医学部, 講師 (10446114)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | KRAP / 結合蛋白 / 癌 / 分子生物学 / 細胞生物学 / トランスジェニックマウス / 結合蛋白質 |
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| Research Abstract |
We generated KRAP-transgenic (TG) mice and identified intracellular signal-related molecule (X) as an associating protein of KRAP by using the TG-liver homogenate. KRAP colocalized with X molecule in both cultured cancer cell lines and mouse physiological tissues. Furthermore, the molecular association between KRAP and X molecules was validated by biochemical analyses. Finally, suppression of KRAP expression in living cells inhibited X-mediated signal transduction. These results showed that KRAP contributes to the regulation of intracellular signal transduction via physical association with X molecule.
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Report
(3 results)
Research Products
(6 results)
