Establishment of KRAP-transgenic mice and elucidation of KRAP function in cancer

• Project/Area Number: 21790331
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Fukuoka University
• Principal Investigator: TAKAHIRO Fujimoto Fukuoka University, 医学部, 講師 (10446114)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: KRAP / 結合蛋白 / 癌 / 分子生物学 / 細胞生物学 / トランスジェニックマウス / 結合蛋白質

Research Abstract

We generated KRAP-transgenic (TG) mice and identified intracellular signal-related molecule (X) as an associating protein of KRAP by using the TG-liver homogenate. KRAP colocalized with X molecule in both cultured cancer cell lines and mouse physiological tissues. Furthermore, the molecular association between KRAP and X molecules was validated by biochemical analyses. Finally, suppression of KRAP expression in living cells inhibited X-mediated signal transduction. These results showed that KRAP contributes to the regulation of intracellular signal transduction via physical association with X molecule.

Research Products

• [Journal Article] Altered energy homeostasis and resistance to diet-induced obesity in KRAP-deficient mice (2009)
  • Author(s): Takahiro Fujimoto
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Presentation] 新たな肥満・糖尿病治療標的としてのKRAP遺伝子 (2010)
  • Author(s): 藤本崇宏、宮坂京子、白澤専二
  • Organizer: 日本人類遺伝学会第55回大会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-10-28
• [Presentation] 新たな肥満・糖尿病治療標的としてのKRAP遺伝子 (2010)
  • Author(s): 藤本崇宏
  • Organizer: 日本人類遺伝学会第55回大会
  • Place of Presentation: 大宮ソニックシティ(埼玉市)
  • Year and Date: 2010-10-28
• [Presentation] 新たな肥満・糖尿病治療標的としてのKRAP遺伝子 (2010)
  • Author(s): 藤本崇宏、白澤専二
  • Organizer: 第2回福岡県医学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-01-31
• [Presentation] Altered Energy Homeostasis and Resistance to Diet-Induced Obesity in KRAP-Deficient Mice (2009)
  • Author(s): 藤本崇宏、宮坂京子、笹月健彦、加藤規弘、白澤専二
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Patent(Industrial Property Rights)] イノシトール-1, 4, 5-トリスリン酸受容体結合タンパク質およびそれを含むトランスジェニック非ヒト哺乳動物 (2009)
  • Inventor(s): 藤本崇宏、白澤専二
  • Industrial Property Rights Holder: 学校法人福岡大学
  • Industrial Property Number: 2009-225596
  • Filing Date: 2009-09-29