Identification of the receptor for Bordetella dermonecrotic toxin.

• Project/Area Number: 21790417
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: FUKUI Aya Osaka University, 微生物病研究所, 助教 (70397743)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ボルデテラ属細菌 / 細菌毒素 / 受容体

Research Abstract

The aim of this project was to identify a receptor for Bordetella dermonecrotic toxin (DNT). I have determined the minimum region of DNT for cell binding, and attempted to isolate the receptor by using the synthetic peptide corresponding to the region. The functional receptor remains unknown and further trials would be needed to identify it. While attempting to purify the receptor, I found that DNT associates with the extracellular matrix. This association may facilitate the toxin action on target cells in vivo.

Research Products

• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki, A., Ohnishi, S., Kamitani, S., Abe, H., Horiguchi, Y.
  • Journal Title: Microbiol Immunol 55 Pages: 154-159
  • Peer Reviewed
• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki A., et al.
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 154-159
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki, A., Kamitani, S., Miyake, M., Horiguchi, Y.
  • Journal Title: BMC Microbiol 10 Pages: 247-255
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. (2010)
  • Author(s): Kamitani S., Fukui-Miyazaki A., et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 285 Pages: 25467-25475
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki A., et al.
  • Journal Title: BMC Microbiology Volume: 10 Pages: 247-255
  • Peer Reviewed
• [Journal Article] Clostridium perfringens enterotoxin interacts with claudins via electrostatic attraction. (2010)
  • Author(s): Kimura J., et al.
  • Journal Title: The Journal of Biological Chemistry 285 Pages: 401-408
  • Peer Reviewed
• [Remarks] ホームページ等
  • URL: http://bactox1.biken.osaka-u.ac.jp/2011Web/Horiguchis_Lab_top.html