Investigation of PE as a novel receptor for botulinum neurotoxin type D
Project/Area Number |
21790430
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | Fujita Health University |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 病原性 / 細菌毒素 / 細菌 / 蛋白質 / P19細胞 / ボツリヌス神経毒素 / 受容体 / ガングリオシド / ボツリヌス菌 / 毒素受容体 |
Research Abstract |
Botulinum neurotoxin type D possesses binding activity to phosphatidylethanolamine(PE), however, it is not unclear whether PE could mediate the internalization of the toxin into neuronal cells. In this study, we determined crystal structure of the receptor binding domain of the toxin. Moreover, we found that mouse embryonic carcinoma P19 cells exhibited high sensitivity to botulinum neurotoxin type C and D/C mosaic toxin, thus making them a novel susceptible cell line for research into botulinum neurotoxins.
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Report
(4 results)
Research Products
(21 results)