| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
In this study, we demonstrated that levels of soluble MD-1 markedly increased with disease progression in sera from MRL/lpr mice. To identify potential sources of serum soluble MD-1 in MRL/lpr mice, we measured the expression of MD-1 mRNA in the spleen, liver and kidney. In the liver and kidney of MRL/lpr mice, levels of MD-1 mRNA increased with age. Furthermore, the expression levels of MD-1 protein in the kidney of MRL/lpr mice increased with age. Finally, we investigated the cell types responsible for MD-1 expression in the kidney of MRL/lpr mice by immunohistochemical analyses. H & E staining of kidney sections revealed that Mac-2-positive macrophages accumulated in the perivascular lesions even at an early stage. More accumulated macrophages were observed in kidneys of 24-week old MRL/lpr mice than 8-week old ones. These cells were also stained with anti-MD-1 polyclonal antibody. These results suggest that macrophages in the kidney express MD-1 and might be a source of serum soluble MD-1 in MRL/lpr mice.
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