Functional analysis of canonical and alternative NF-κB pathways in mature B lymphocyte differentiation.
| Project/Area Number |
21790483
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SASAKI Yoshiteru The Institute of Physical and Chemical Research, 幹細胞研究グループ, 研究員 (80323004)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 転写因子 / Bリンパ球 / 発生・維持 / トランスジェニックマウス / ノックアウトマウス / NF-κB / サイトカイン / NF-_κB |
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| Research Abstract |
NF-κB pathway is activated mainly in two ways, canonical and alternative pathways. Gene-targeting studies show that both canonical and alternative pathways are essential for mature B cell development. However it is a little known how these pathways controls the development of mature B cells. I established conditional knock-in transgenes of p52NF-κB2 and crossed with NEMO deficient mice. The expression of p52NF-κB2 rescues the development of NEMO deficient follicular and marginal zone B cells but not B1 cells. This result shows that canonical and alternative NF-κB pathways control common target genes in resting follicular and canonical NF-kB pathway in itself is essential for the development of B1 cells.
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Report
(3 results)
Research Products
(6 results)
