Functional analysis of canonical and alternative NF-κB pathways in mature B lymphocyte differentiation.

• Project/Area Number: 21790483
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SASAKI Yoshiteru The Institute of Physical and Chemical Research, 幹細胞研究グループ, 研究員 (80323004)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 転写因子 / Bリンパ球 / 発生・維持 / トランスジェニックマウス / ノックアウトマウス / NF-κB / サイトカイン / NF-_κB

Research Abstract

NF-κB pathway is activated mainly in two ways, canonical and alternative pathways. Gene-targeting studies show that both canonical and alternative pathways are essential for mature B cell development. However it is a little known how these pathways controls the development of mature B cells. I established conditional knock-in transgenes of p52NF-κB2 and crossed with NEMO deficient mice. The expression of p52NF-κB2 rescues the development of NEMO deficient follicular and marginal zone B cells but not B1 cells. This result shows that canonical and alternative NF-κB pathways control common target genes in resting follicular and canonical NF-kB pathway in itself is essential for the development of B1 cells.

Research Products

• [Journal Article] B細胞抗原受容体(BCR)からの成熟B細胞生存維持シグナル (2011)
  • Author(s): 佐々木義輝
  • Journal Title: 細胞工学 30 Pages: 60-65
  • NAID: 40017652605
• [Journal Article] Movement of BCR in the plasma membrane for generating tonic signal. (2010)
  • Author(s): Sasaki, Y., Kurosaki, T.
  • Journal Title: Immunity 32 Pages: 143-144
• [Presentation] Generation and analysis of B cell-specific p52NF-κB2 transgenic mice (2010)
  • Author(s): 佐々木義輝
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場
  • Year and Date: 2010-08-24
• [Presentation] Generation and analysis of B cell-specific p52NF-κB2 transgenic mice (2010)
  • Author(s): 佐々木義輝
  • Organizer: 第14回 国際免疫学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-24
• [Presentation] Molecular mechanism for maintenance of mature B cells mediated by BCR and NF-κB (2009)
  • Author(s): 佐々木義輝
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪府大阪市大阪国際会議場(グランキューブ大阪)
  • Year and Date: 2009-12-04
• [Remarks] ホームページ
  • URL: http://www.cdb.riken.go.jp/scb/